Human TIMP-1 Antibody

(13 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human TIMP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) TIMP‑1 or rhTIMP‑2.
  • Source
    Monoclonal Mouse IgG2B Clone # 63515
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TIMP-1
    Cys24-Ala207
    Accession # P01033
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TIMP-1 Western Blot Standard (Catalog # WBC021)
    under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    Human T cells fixed with paraformaldehyde and permeabilized with saponin
    • Human TIMP-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human TIMP‑1 Antibody (Catalog # MAB970)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human TIMP‑1 Biotinylated Antibody (Catalog # BAF970)
  • ELISA Standard
     
    Recombinant Human TIMP-1 Protein, CF (Catalog # 970-TM)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TIMP‑1 in Human Astrocytoma. TIMP‑1 was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with Pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.

  • Long Name:
    Tissue Inhibitors of Metalloproteinases 1
  • Entrez Gene IDs:
    7076 (Human); 21857 (Mouse); 116510 (Rat)
  • Alternate Names:
    CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Increased expression of matrix metalloproteinase-1 in systemic vessels of preeclamptic women: a critical mediator of vascular dysfunction.
    Authors: Estrada-Gutierrez G, Cappello RE, Mishra N, Romero R, Strauss JF, Walsh SW
    Am. J. Pathol., 2011;178(1):451-60.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Luminex Development
  3. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010;82(5):968-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell-based ELISA
  4. Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
    Authors: Cavallo C, Desando G, Facchini A, Grigolo B
    J Biomed Mater Res A, 2010;93(1):86-95.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
    Authors: Lucio-Eterovic AK, Piao Y, de Groot JF
    Clin. Cancer Res., 2009;15(14):4589-99.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  6. Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
    Authors: Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
    Exp. Hematol., 2009;37(7):849-56.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  7. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2009;6(1):9-17.
    Species: Human
    Sample Type: Tissue Secretion
    Application: WB
  8. Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
    Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
    Mol. Cell Proteomics, 2008;7(10):1974-82.
    Species: Human
    Sample Type: Serum
    Application: MS
  9. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
    Authors: Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
    J. Immunol., 2007;178(9):5957-65.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  10. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
    Authors: Naveau A, Reinald N, Fournier B
    Connect. Tissue Res., 2007;48(6):300-8.
    Species: Human
    Sample Type: Whole Cells
    Application: DB
  11. Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
    Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
    Gut, 2006;55(12):1774-80.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing.
    Authors: Djafarzadeh R, Noessner E, Engelmann H
    Oncogene, 2006;25(10):1496-508.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  13. Limited processing of pro-matrix metalloprotease-2 (gelatinase A) overexpressed by transfection in PC-3 human prostate tumor cells: association with restricted cell surface localization of membrane-type matrix metalloproteinase-1.
    Authors: Wilson MJ, Jiang A, Wiehr C, Wang X, Sinha AA, Pei D
    J. Androl., 2004;25(2):274-85.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
Expand to show all 13 Citations
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Human.
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ELISA Human Anonymous 12/06/2017
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Summary

ApplicationELISA
Sample TestedEDTA Plasma
SpeciesHuman
ELISA Human TIMP-1 Antibody MAB970
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Excellent
 ELISA Human Anonymous 11/16/2017
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ELISA Human TIMP-1 Antibody MAB970
ELISA: Human TIMP-1 Antibody [MAB970]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis antibody was paired with BAF970 to build an ELISA. The assay was used to measure TIMP-1 in human serum samples.

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