Human TIMP-1 DuoSet ELISA

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Human TIMP-1 ELISA Standard Curve
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Product Details
Citations (38)
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Human TIMP-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TIMP-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Human TIMP-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIMP-1

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name:
Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs:
7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names:
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

Assay Procedure


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human TIMP-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Mg-based materials diminish tumor spreading and cancer metastases
    Authors: P Globig, R Madurawala, R Willumeit-, F Martini, E Mazzoni, BJC Luthringer
    Bioactive materials, 2023;19(0):594-610.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Association between Circulating Levels of 25-Hydroxyvitamin D3 and Matrix Metalloproteinase-10 (MMP-10) in Patients with Type 2 Diabetes
    Authors: D Abasheva, MM Dolcet-Neg, MA Fernández-, JM Mora-Gutié, J Orbe, FJ Escalada, N Garcia-Fer
    Nutrients, 2022;14(17):.
    Species: Human
    Sample Types: Serum
  3. Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
    Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Journal of ovarian research, 2022;15(1):87.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Evaluation in a Cytokine Storm Model In Vivo of the Safety and Efficacy of Intravenous Administration of PRS CK STORM (Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells)
    Authors: JP Lapuente, G Gómez, J Marco-Brua, P Fernández, P Desportes, J Sanz, M García-Gil, F Bermejo, JV San Martín, A Algaba, JC De Gregori, D Lapuente, A De Gregori, B Lapuente, S Gómez, MLV Andrés, A Anel
    Biomedicines, 2022;10(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. A combined biomarker approach for characterising extracellular matrix profiles in acute myocardial infarction
    Authors: MM Brunton-O', AS Holley, KE Hally, GA Kristono, SA Harding, PD Larsen
    Scientific Reports, 2021;11(1):12705.
    Species: Human
    Sample Types: Plasma
  6. The Polymethoxy Flavonoid Sudachitin Inhibits Interleukin-1&beta-Induced Inflammatory Mediator Production in Human Periodontal Ligament Cells
    Authors: Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo
    BioMed Research International, 2021;2021(0):8826586.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Antibody microarray analysis of the amniotic fluid proteome�for predicting the outcome of rescue cerclage in patients with cervical insufficiency
    Authors: S Hong, KH Park, YE Lee, S Shin, HJ Kim, YM Kim
    Bioscience Reports, 2021;41(7):.
    Species: Human
    Sample Types: Amniotic Fluid
  8. Potential novel biomarkers for chronic lung allograft dysfunction and azithromycin responsive allograft dysfunction
    Authors: C Veraar, J Kliman, A Benazzo, F Oberndorfe, M Laggner, P Hacker, T Raunegger, S Janik, P Jaksch, W Klepetko, HJ Ankersmit, B Moser
    Scientific Reports, 2021;11(1):6799.
    Species: Human
    Sample Types: Serum
  9. MMPs and TIMPs levels are correlated with anthropometric parameters, blood pressure, and endothelial function in obesity
    Authors: S Boumiza, K Chahed, Z Tabka, MP Jacob, X Norel, G Ozen
    Scientific Reports, 2021;11(1):20052.
    Species: Human
    Sample Types: Plasma
  10. Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma
    Authors: R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut?
    Scientific Reports, 2021;11(1):13100.
    Species: Human
    Sample Types: Serum
  11. Differential inflammation-mediated function of prokineticin 2 in the synovial fibroblasts of patients with rheumatoid arthritis compared with osteoarthritis
    Authors: K Noda, B Dufner, H Ito, K Yoshida, G Balboni, RH Straub
    Scientific Reports, 2021;11(1):18399.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Atypical response to bacterial co-infection and persistent neutrophilic broncho-alveolar inflammation distinguish critical COVID-19 from influenza
    Authors: S Cambier, M Metzemaeke, AC Carvalho, A Nooyens, C Jacobs, L Vanderbeke, B Malengier-, M Gouwy, E Heylen, P Meersseman, G Hermans, E Wauters, A Wilmer, C Consortium, D Schols, P Matthys, G Opdenakker, RE Marques, J Wauters, J Vandooren, P Proost
    JCI Insight, 2021;0(0):.
    Species: Human
    Sample Types: BAL Supernatant
  13. Nobiletin Decreases Inflammatory Mediator Expression in Tumor Necrosis Factor-Stimulated Human Periodontal Ligament Cells
    Authors: Y Hosokawa, I Hosokawa, K Ozaki
    Mediators of Inflammation, 2021;2021(0):5535844.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Matrix metalloproteinase-7 could be a predictor for acute inflammation in psoriatic patients
    Authors: A Abbes, Y Zayani, W Zidi, MB Hammami, A Mebazaa, D El Euch, A Ben Ammar, H Sanhaji, MV El May, M Mokni, M Feki, M Allal-Elas
    Cytokine, 2020;134(0):155195.
    Species: Human
    Sample Types: Plasma
  15. Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease
    Authors: JB Trojanek, J Micha?kiew, R Grzywa-Czu, W Ja?czyk, L Gackowska, I Kubiszewsk, A Helmin-Bas, A Wierzbicka, M Szalecki, P Socha
    Mediators Inflamm., 2020;2020(0):8327945.
    Species: Human
    Sample Types: Plasma
  16. Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients
    Authors: HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas
    PLoS ONE, 2020;15(12):e0243759.
    Species: Human
    Sample Types: Plasma
  17. Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes: An in�vitro study using baicalein
    Authors: CC Wu, YR Chen, DH Lu, LH Hsu, KC Yang, S Sumi
    Regen Ther, 2020;14(0):177-183.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. MMP-10 is Increased in Early Stage Diabetic Kidney Disease and can be Reduced by Renin-Angiotensin System Blockade
    Authors: JM Mora-Gutié, JA Rodríguez, MA Fernández-, J Orbe, FJ Escalada, MJ Soler, MF Slon Roble, M Riera, JA Páramo, N Garcia-Fer
    Sci Rep, 2020;10(1):26.
    Species: Human
    Sample Types: Serum
  19. Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants
    Authors: YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park
    Invest. Ophthalmol. Vis. Sci., 2019;60(12):3813-3820.
    Species: Human
    Sample Types: Plasma
  20. Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response
    Authors: S Coufal, N Galanova, L Bajer, Z Gajdarova, D Schierova, Z Jiraskova, K Kostovciko, Z Jackova, Z Stehlikova, P Drastich, H Tlaskalova, M Kverka
    Cells, 2019;8(7):.
    Species: Human
    Sample Types: Serum
  21. Elevated systemic levels of the matrix metalloproteinase inhibitor TIMP-1 correlate with clinical markers of cachexia in patients with chronic pancreatitis and pancreatic cancer
    Authors: O Prokopchuk, B Grünwald, U Nitsche, C Jäger, OL Prokopchuk, EC Schubert, H Friess, ME Martignoni, A Krüger
    BMC Cancer, 2018;18(1):128.
    Species: Human
    Sample Types: Plasma
  22. Proteolytic biomarkers are related to prognosis in COPD- report from a population-based cohort
    Authors: R Linder, E Rönmark, J Pourazar, AF Behndig, A Blomberg, A Lindberg
    Respir. Res., 2018;19(1):64.
    Species: Human
    Sample Types: Serum
  23. Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis
    Authors: S Brilha, CWM Ong, B Weksler, N Romero, PO Couraud, JS Friedland
    Sci Rep, 2017;7(1):16031.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Complex regulation of neutrophil-derived MMP-9 secretion in central nervous system tuberculosis
    Authors: CW Ong, PJ Pabisiak, S Brilha, P Singh, F Roncaroli, PT Elkington, JS Friedland
    J Neuroinflammation, 2017;14(1):31.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Association of Matrix Metalloproteinases -7, -8 and -9 and TIMP -1 with Disease Severity in Acute Pancreatitis. A Cohort Study
    PLoS ONE, 2016;11(8):e0161480.
    Species: Human
    Sample Types: Plasma
  26. MMP-1 Activation Contributes to Airway Smooth Muscle Growth and Asthma Severity
    Authors: Shams-Un-Nisa Naveed
    Am. J. Respir. Crit. Care Med, 2016;0(0):.
    Species: Human
    Sample Types: BALF
  27. Serum metalloproteinase-9 is related to COPD severity and symptoms - cross-sectional data from a population based cohort-study.
    Authors: Linder R, Ronmark E, Pourazar J, Behndig A, Blomberg A, Lindberg A
    Respir Res, 2015;16(0):28.
    Species: Human
    Sample Types: Serum
  28. Decreased PGE(2) content reduces MMP-1 activity and consequently increases collagen density in human varicose vein.
    Authors: Gomez I, Benyahia C, Louedec L, Leseche G, Jacob M, Longrois D, Norel X
    PLoS ONE, 2014;9(2):e88021.
    Species: Human
    Sample Types: Tissue Culture Supernates
  29. Compartment differences of inflammatory activity in chronic obstructive pulmonary disease.
    Authors: Ji J, von Scheele I, Bergstrom J, Billing B, Dahlen B, Lantz A, Larsson K, Palmberg L
    Respir Res, 2014;15(0):104.
    Species: Human
    Sample Types: Serum
  30. Identification of neutrophil activation markers as novel surrogate markers of CF lung disease.
    Authors: Rath T, Zwaschka L, Hage L, Kugler M, Menendez K, Naehrlich L, Schulz R, Roderfeld M, Roeb E
    PLoS ONE, 2014;9(12):e115847.
    Species: Human
    Sample Types: Serum
  31. Expression of matrix metalloproteinases and their tissue inhibitors in the serum and cerebrospinal fluid of patients with HIV-1 infection and syphilis or neurosyphilis.
    Authors: Tsai HC, Ye SY, Kunin CM, Lee SS, Wann SR, Tai MH, Shi MH, Liu YC, Chen YS
    Cytokine, 2011;54(0):109-16.
    Species: Human
    Sample Types: CSF
  32. STAT3, p38 MAPK, and NF-kappaB drive unopposed monocyte-dependent fibroblast MMP-1 secretion in tuberculosis.
    Authors: O'Kane CM, Elkington PT, Jones MD, Caviedes L, Tovar M, Gilman RH, Stamp G, Friedland JS
    Am. J. Respir. Cell Mol. Biol., 2010;43(0):465.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers.
    Authors: Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF
    Am. J. Respir. Crit. Care Med., 2009;179(12):1107-14.
    Species: Human
    Sample Types: BALF
  34. Association of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-4 in cerebrospinal fluid with blood-brain barrier dysfunction in patients with eosinophilic meningitis caused by Angiostrongylus cantonensis.
    Authors: Tsai HC, Chung LY, Chen ER, Sy CL
    Am. J. Trop. Med. Hyg., 2008;78(1):20-7.
    Species: Human
    Sample Types: CSF
  35. Matrix metalloproteinases and their tissue inhibitors (TIMPs) in Plasmodium falciparum malaria: serum levels of TIMP-1 are associated with disease severity.
    Authors: Dietmann A, Helbok R, Lackner P, Issifou S, Lell B, Matsiegui PB, Reindl M, Schmutzhard E, Kremsner PG
    J. Infect. Dis., 2008;197(11):1614-20.
    Species: Human
    Sample Types: Serum
  36. Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis.
    Authors: Lauder SN, Carty SM, Carpenter CE
    Rheumatology (Oxford), 2007;46(5):752-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Increased production of matrix metalloproteinases in Helicobacter pylori-associated human gastritis.
    Authors: Bergin PJ, Anders E, Sicheng W, Erik J, Jennie A, Hans L, Pierre M, Qiang PH, Marianne QJ
    Helicobacter, 2004;9(0):201.
    Species: Human
    Sample Types: Tissue Homogenates
  38. IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation.
    Authors: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S
    Int J Mol Sci, 0;23(17):.
    Species: Human
    Sample Types: Tissue Culture Supernates


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