Human TIN-Ag Antibody

Catalog # Availability Size / Price Qty
Detection of Human TIN-Ag by Western Blot.
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Product Details
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Human TIN-Ag Antibody Summary

Species Reactivity
Detects human TIN-Ag in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TINAGL1 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant human TIN-Ag
Accession # Q6NSC1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human TIN-Ag antibody by Western Blot. View Larger

Detection of Human TIN-Ag by Western Blot. Western blot shows lysates of human kidney (cortex and medulla) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human TIN-Ag Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIN-Ag at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIN-Ag

TIN-Ag (Tubulointerstitial nephritis antigen) is a 48-58 kDa glycoprotein member of the peptidase C1 family of molecules. It is secreted by renal tubule epithelium and (presumably) small intestine columnar epithelium. TIN-Ag is an integral component of the renal tubule basement membrane (BM), and appears to promote proper BM matrix organization, block laminin polymerization, and serve as a receptor for the epithelial integrins alpha 3 beta 1 and alpha v beta 3. Epithelial dissociation from the BM is associated with an epithelial-to-mesenchymal transition. Human TIN-Ag is synthesized as a 476 amino acid (aa) preproprecursor. It contains a 19 aa signal sequence, a 30 aa furin-cleavable prosegment (aa 20-49), and a 427 aa mature region (aa 50-476). Within the mature region is an SMB domain (aa 61-106), one vWFC domain (aa 119‑154), and a nonenzymatic peptidase C1A region (aa 218-466). Multiple splice variants are possible, and potential isoforms may show an alternative start site at Met322, or a combined deletion of aa 119‑169 plus 209-300, or a combination of an alternative start site at Met19 coupled to a 13 aa substitution for aa 209-476. Over aa 20-476, human proTIN-Ag shares 86% aa identity with mouse proTIN-Ag; over the mature region (aa 50-476), human TIN-Ag shares 89% aa identity with mouse TIN-Ag.

Long Name
Tubulointerstitial Nephritis Antigen
Entrez Gene IDs
27283 (Human); 26944 (Mouse); 300846 (Rat)
Alternate Names
TIN1; TIN2; TINAg; TIN-Ag; tubulointerstitial nephritis antigen

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