Human TIN-Ag Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TIN-Ag in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TINAGL1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human TIN-Ag
    Glu20-Pro476
    Accession # Q6NSC1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TIN-Ag by Western Blot. Western blot shows lysates of human kidney (cortex and medulla) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human TIN-Ag Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6797) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TIN-Ag at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIN-Ag

TIN-Ag (Tubulointerstitial nephritis antigen) is a 48-58 kDa glycoprotein member of the peptidase C1 family of molecules. It is secreted by renal tubule epithelium and (presumably) small intestine columnar epithelium. TIN-Ag is an integral component of the renal tubule basement membrane (BM), and appears to promote proper BM matrix organization, block laminin polymerization, and serve as a receptor for the epithelial integrins alpha 3 beta 1 and alpha v beta 3. Epithelial dissociation from the BM is associated with an epithelial-to-mesenchymal transition. Human TIN-Ag is synthesized as a 476 amino acid (aa) preproprecursor. It contains a 19 aa signal sequence, a 30 aa furin-cleavable prosegment (aa 20-49), and a 427 aa mature region (aa 50-476). Within the mature region is an SMB domain (aa 61-106), one vWFC domain (aa 119‑154), and a nonenzymatic peptidase C1A region (aa 218-466). Multiple splice variants are possible, and potential isoforms may show an alternative start site at Met322, or a combined deletion of aa 119‑169 plus 209-300, or a combination of an alternative start site at Met19 coupled to a 13 aa substitution for aa 209-476. Over aa 20-476, human proTIN-Ag shares 86% aa identity with mouse proTIN-Ag; over the mature region (aa 50-476), human TIN-Ag shares 89% aa identity with mouse TIN-Ag.

  • Long Name:
    Tubulointerstitial Nephritis Antigen
  • Entrez Gene IDs:
    27283 (Human); 26944 (Mouse); 300846 (Rat)
  • Alternate Names:
    TIN1; TIN2; TINAg; TIN-Ag; tubulointerstitial nephritis antigen
Related Research Areas
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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