|Detection of Human TINAGL1 by Western Blot. Western blot shows lysates of human aorta tissue, human placenta tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human TINAGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7185) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TINAGL1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
TINAGL1 (Tubulointerstitial nephritis antigen-like 1; also TIN-ag-RP, AZ-1 and LCN7) is a 54-57 kDa glycoprotein member of the peptidase C1 family of molecules. It is secreted by renal tubule epithelium, zona glomerulosa cells, vascular smooth muscle and striated muscle cells. TINAGL1 may play a role in the spatial and temporal development of the steroid component of the adrenal, in part by suppressing the expression of CYP11B1. Human TINAGL1 is synthesized as a 467 amino acid (aa) precursor. It contains a 21 aa signal sequence and a 446 aa mature region (aa 22-467). Within the mature region is an SMB (somatomedin B) domain (aa 50-98), one vWFC domain (aa 105-140), and a nonenzymatic peptidase C1A region (aa 204-455). There are multiple potential isoform variants. One contains an alternative start site at Met106, another shows a deletion of aa 126-156, and a third possesses a 60 aa substitution for aa 1-194, coupled to a 38 aa insertion after Gly286, and a 16 aa substitution for aa 365-467. A 40-42 kDa and 22 kDa undefined form of TINAGL1 has been noted in transfected cell supernatant subjected to SDS-PAGE. Over aa 22‑464, human TINAGL1 shares 90% aa identity with mouse TINAGL1.
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