Human TL1A/TNFSF15 Antibody

Catalog # Availability Size / Price Qty
AF744
AF744-SP

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TL1A/TNFSF15 in Human Prostate.
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Human TL1A/TNFSF15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TL1A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant mouse TL1A is observed, and less than 2% cross‑reactivity with recombinant human (rh) APRIL, rhTNF-alpha, and rhFas Ligand is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TL1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TL1A/TNFSF15 (Catalog # 1319-TL)
Immunohistochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TL1A/TNFSF15 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

TL1A/TNFSF15 in Human Prostate. TL1A/TNFSF15 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human TL1A/TNFSF15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF744) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TL1A/TNFSF15

TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15). Human TL1A is a 251 aa protein consisting of a 35 aa cytoplasmic domain, a 24 aa transmembrane region and a 192 aa C-terminal extracellular domain. It is a longer variant of the previously cloned TL1 (also known as VEGI) that is possibly a cloning artifact. TL1A is predominantly expressed in endothelial cells and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to death receptor 3 (DR3), which is now designated TNF receptor superfamily member 25 (TNFRSF25). DR3 was formerly designated TNFRSF12 when it was thought to be the receptor for TWEAK/TNFSF12. DR3 is expressed primarily on activated T cells. Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways, activation of the transcription factor NF-kappa-B or activation of caspases and apoptosis. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. These effects of TL1A are blocked by the secreted, soluble decoy receptor 3 (DcR3), also known as TR6 and TNFRSF6B, which compete with DR3 for binding to TL1A. Consistent with the observed in vitro activities, TL1A promotes ex vivo splenocyte expansion and enhances in vivo graft-versus-host-response.

References
  1. Migone, T.S. et al. (2002) Immunity 16:479.
Long Name
TNF-like 1
Entrez Gene IDs
9966 (Human); 326623 (Mouse)
Alternate Names
MGC129934; MGC129935; TL1A; TL1Vascular endothelial cell growth inhibitor; TNF superfamily ligand TL1A; TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; vascular endothelial growth inhibitor-192A; VEGI; VEGI192A; VEGITNF ligand-related molecule 1

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Citation for Human TL1A/TNFSF15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The somatostatin analogue octreotide inhibits growth of small intestine neuroendocrine tumour cells.
    Authors: Li S, Martijn C, Cui T, Essaghir A, Luque R, Demoulin J, Castano J, Oberg K, Giandomenico V
    PLoS ONE, 2012-10-31;7(10):e48411.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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