Human TLR7 Alexa Fluor® 647-conjugated Antibody

Name: Human TLR7 Alexa Fluor® 647-conjugated Antibody
Brand: R&D Systems

Datasheet / CoA / SDS

Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry.

Intracellular Staining by Flow Cytometry

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Product Details

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Species Reactivity

Human
Specificity

Detects human TLR7 in direct ELISAs.
Source

Monoclonal Mouse IgG_2A Clone # 533707
Purification

Protein A or G purified from hybridoma culture supernatant
Immunogen

E. coli-derived recombinant human TLR7
Met360-Leu516
Accession # Q9NYK1
Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label

Alexa Fluor 647

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Intracellular Staining by Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry

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Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC5875R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR7

Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.

Long Name:

Toll-like Receptor 7

Entrez Gene IDs:

51284 (Human); 170743 (Mouse); 317468 (Rat)

Alternate Names:

TLR7; toll-like receptor 7

Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

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