Human TLR7 Alexa Fluor® 647-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry View Larger

Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC5875R, filled histogram) or isotype control antibody (Catalog # IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins.

Intracellular Staining by Flow Cytometry View Larger

Detection of TLR7 in RPMI8226 Human Cell Line by Flow Cytometry. RPMI8226 human myeloma cell line was stained with Mouse Anti-Human TLR7 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC5875R, filled histogram) or isotype control antibody (IC003R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Membrane-associated Proteins protocol.

Preparation and Storage

Background: TLR7

Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.

Product Datasheets

Related Research Areas

• IL-1 Family
• Immune Regulatory Molecules on Endothelial Cells
• NFkB Pathway
• NOD-like Receptors and the Inflammasome
• Pattern Recognition Receptors
• Plasmacytoid Dendritic Cells
• Regulatory T Cells (Tregs)
• Toll-Like Receptors