Human TLR7 PerCP-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human TLR7 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 533707
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TLR7
    Accession # Q9NYK1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry. Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PerCP‑conjugated Monoclonal Antibody (Catalog # IC5875C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TLR7
Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.
  • Long Name:
    Toll-like Receptor 7
  • Entrez Gene IDs:
    51284 (Human); 170743 (Mouse); 317468 (Rat)
  • Alternate Names:
    toll-like receptor 7
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Hepatitis C Virus Sensing by Human Trophoblasts Induces Innate Immune Responses and Recruitment of Maternal NK Cells: Potential Implications for Limiting Vertical Transmission.
    Authors: Giugliano S, Petroff M, Warren B, Jasti S, Linscheid C, Ward A, Kramer A, Dobrinskikh E, Sheiko M, Gale M, Golden-Mason L, Winn V, Rosen H
    J Immunol, 2015;195(8):3737-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PerCP-conjugated Isotype Control

Ctrl IC003C
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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