Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunohistochemistry-Frozen, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 533707
View all TLR7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TLR7
Met360-Leu516
Accession # Q9NYK1

Specificity

Detects human TLR7 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TLR7 PE‑conjugated Antibody

Detection of TLR7 antibody in Ramos Human Cell Line antibody by Flow Cytometry.

Detection of TLR7 in Ramos Human Cell Line by Flow Cytometry.

Ramos human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TLR7 PE-conjugated Monoclonal Antibody (Catalog # IC5875P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human TLR7 by Flow Cytometry

Detection of Human TLR7 by Flow Cytometry

Gating strategy for the detection of TLR-7 and -9 in B cell subsets.PBMC were isolated from whole blood and stained for surface markers before cells were fixed, permeabilised and stained for TLR-7 and -9. FSC and SSC were first used to gate out debris (A) and SSC-A and SSC-H was utilized to eliminate duplicates (B). Further gating was done on CD45 and CD19, to target B cells (C). To separate between the different B cell populations, we gated on CD27 and IgD (D), followed by TLR-7 and -9 expression on these subsets (E). Data from one representative patient is shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0120383), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TLR7 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Ramos human Burkitt's lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 4 using IC5875P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR7

Toll-like receptor 7 (TLR7) is a 120 kDa (predicted, unglycosylated) type I transmembrane glycoprotein and member of the Toll-like receptor family. Human TLR7 is synthesized as a 1049 amino acid (aa) precursor that contains a 26 aa signal sequence, an 803 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 189 aa cytoplasmic domain. Mature human TLR7 is 81% aa identical to mature mouse TLR7. TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung, and in plasmacytoid pre-dendritic cells. Functionally, TLR7 participates in the innate immune response to microbial agents.

Long Name

Toll-like Receptor 7

Alternate Names

toll-like receptor 7

Entrez Gene IDs

51284 (Human); 170743 (Mouse); 317468 (Rat)

Gene Symbol

TLR7

UniProt

Q9NYK1

Additional TLR7 Products

Product Documents for Human TLR7 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TLR7 PE‑conjugated Antibody

For research use only

Citations for Human TLR7 PE‑conjugated Antibody

Customer Reviews for Human TLR7 PE‑conjugated Antibody (1)

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  • Human TLR7 PE-conjugated Antibody
    Name: Stephane Hua
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 07/05/2018

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