Human TNF RI/TNFRSF1A Biotinylated Antibody
Human TNF RI/TNFRSF1A Biotinylated Antibody Summary
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Applications
Human TNF RI/TNFRSF1A Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A
TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily member, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF- alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1 - 3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6 - 8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain(5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of human TNF RI shows 80%, 80%, 73%, 69% and 70% aa identity with dog, cat, pig, rat and mouse TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.
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- Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
- Legler, D.F. et al. (2003) Immunity 18:655.
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- Schall, T.J. et al. (1990) Cell 61:361.
Product Datasheets
Citations for Human TNF RI/TNFRSF1A Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Inflammation-Driven Regulation of PD-L1 and PD-L2, and Their Cross-Interactions with Protective Soluble TNFalpha Receptors in Human Triple-Negative Breast Cancer
Authors: T Baram, N Oren, N Erlichman, T Meshel, A Ben-Baruch
Cancers, 2022;14(14):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2.
Authors: Inoue M, Kamada H, Abe Y, Higashisaka K, Nagano K, Mukai Y, Yoshioka Y, Tsutsumi Y, Tsunoda S
J Cell Sci, 2015;128(4):656-69.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development -
Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production.
Authors: Emsley HC, Smith CJ, Gavin CM
BMC Neurol, 2007;7(0):5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004;284(1):99-106.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Array Development -
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis.
Authors: Bharucha AE, 2019, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD
, 2000;95(9):2338-42.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder.
Authors: Jyonouchi H, Sun S, Itokazu N
Neuropsychobiology, 0;46(2):76-84.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
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Reviews for Human TNF RI/TNFRSF1A Biotinylated Antibody
Average Rating: 5 (Based on 1 Review)
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We used MAB625 as the solid phase antibody, 636-R1 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.
