Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, Antibody Array Development, Array Development, ELISA Capture, ELISA Development, Proximity Ligation Assay (PLA)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TNF RI/TNFRSF1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human sTNF RI

Specificity

Detects human TNF RI in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant human (rh) TNF‑ alpha, recombinant mouse (rm) TNF‑ alpha, recombinant porcine TNF‑ alpha, recombinant rat TNF‑ alpha, rhTNF‑ beta, rmsTNF RI, rhsTNF RII, and rmsTNF RII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human TNF RI/TNFRSF1A Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)

Human TNF RI/TNFRSF1A Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human TNF RI/TNFRSF1A Antibody (Catalog # MAB625)
  • Standard: Recombinant Human sTNF RI/TNFRSF1A Protein (Catalog # 636-R1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF225 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a type I transmembrane protein member of the TNF receptor superfamily member, designated TNFRSF1A (1, 2). Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-  alpha trimer-binding cysteine-rich domains (CRD) in their extracellular domains (ECD). However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1 - 3). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (4). TNF RI is present on the cell surface as a trimer of 55 kDa subunits (4, 5). TNF-alpha induces sequestering of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (9, 10). Release of the 28 - 34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 6 - 8). Full-length TNF RI may also be released in exosome-like vesicles (11). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 12, 13). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Human TNF RI is a 455 amino acid (aa) protein that contains a 21 aa signal sequence, a 190 aa ECD with a PLAD domain(5) that mediates constitutive trimer formation, followed by the four CRD, a 23 aa transmembrane domain, and a 221 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (15). The ECD of human TNF RI shows 80%, 80%, 73%, 69% and 70% aa identity with dog, cat, pig, rat and mouse TNF RI, respectively; it shows 23% aa identity with the ECD of TNF RII.

References

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73: 431.
  5. Chan, F.K. et al. (2000) Science 288:2351.
  6. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  7. Jin, L. et al. (2000) J. Immunol. 165:5153.
  8. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  9. Legler, D.F. et al. (2003) Immunity 18:655.
  10. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  11. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  12. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  13. McDermott, M.F. et al. (1999) Cell 97:133.
  14. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  15. Schall, T.J. et al. (1990) Cell 61:361.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1A

Additional TNF RI/TNFRSF1A Products

Product Documents for Human TNF RI/TNFRSF1A Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TNF RI/TNFRSF1A Biotinylated Antibody

For research use only

Citations for Human TNF RI/TNFRSF1A Biotinylated Antibody

Customer Reviews for Human TNF RI/TNFRSF1A Biotinylated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human TNF RI/TNFRSF1A Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma and Cell culture supernatant
    Species: Human
    Verified Customer | Posted 11/22/2017
    We used MAB625 as the solid phase antibody, 636-R1 as the immunoassay standard and BAF225 as the detection antibody in a sandwich ELISA. Everything worked very well.
    Human TNF RI/TNFRSF1A Biotinylated Antibody BAF225
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 22226222
    Species: Human
    Verified Customer | Posted 01/12/2015

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