Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Tollip Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Tollip
Met1-Pro274
Accession # Q9H0E2

Specificity

Detects endogenous human Tollip in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Tollip Antibody

Detection of Human Tollip antibody by Western Blot.

Detection of Human Tollip by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Tollip Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Tollip at approximately 30 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 4.

Applications for Human Tollip Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tollip

Tollip (Toll-interacting protein) is a 30 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids (aa) in length. It contains a C2 Ca++-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa’s 6‑33. Human Tollip is 92% identical to mouse and canine Tollip.

Long Name

Toll Interacting Protein

Alternate Names

IL-1RAcPIP

Entrez Gene IDs

54472 (Human); 54473 (Mouse); 361677 (Rat)

Gene Symbol

TOLLIP

UniProt

Q9H0E2 (Human)

Additional Tollip Products

Product Documents for Human Tollip Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Tollip Antibody

For research use only

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