Human Total Adiponectin/Acrp30 Quantikine ELISA Kit

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SRP300
PDRP300
DRP300
Control Products Available
Adiponectin/Acrp30 ELISA Kit ELISA
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Procedure
Citations (103)
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Human Total Adiponectin/Acrp30 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.891 ng/mL
Assay Range
3.9 - 250 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human total Adiponectin (low, middle, and high molecular weight)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Total Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total (low, middle, and high molecular weight) human Adiponectin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Adiponectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Adiponectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 12.5 45.3 91.5 12.7 47.1 100
Standard Deviation 0.41 1.27 4.56 1 2.8 6.7
CV% 3.2 2.8 5 7.9 5.9 6.7

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 19.8 69.9 143 20.5 74.4 157
Standard Deviation 0.5 2.4 6.76 1.4 4.3 10.8
CV% 2.5 3.4 4.7 6.8 5.8 6.9

Recovery

The recovery of Adiponectin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 88-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Adiponectin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Total Adiponectin/Acrp30 ELISA Linearity

Data Examples

Human Total Adiponectin/Acrp30 ELISA Cell Culture Supernate Standard Curve

Human Total Adiponectin/Acrp30 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Entrez Gene IDs:
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names:
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well.
  13.   For Cell Culture Supernate Samples: Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    For Serum & Plasma Samples: Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Total Adiponectin/Acrp30 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

103 Citations: Showing 1 - 10
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  1. Daily walnut intake improves metabolic syndrome status and increases circulating adiponectin levels: randomized controlled crossover trial
    Authors: HJ Hwang, Y Liu, HS Kim, H Lee, Y Lim, H Park
    Nutr Res Pract, 2019;13(2):105-114.
    Species: Human
    Sample Types: Serum
  2. Adiponectin and insulin resistance are related to restenosis and overall new PCI in subjects with normal glucose tolerance: the prospective AIRE Study
    Authors: FC Sasso, PC Pafundi, R Marfella, P Calabrò, F Piscione, F Furbatto, G Esposito, R Galiero, F Gragnano, L Rinaldi, T Salvatore, M D'Amico, LE Adinolfi, C Sardu
    Cardiovasc Diabetol, 2019;18(1):24.
    Species: Human
    Sample Types: Plasma
  3. Impaired mitochondrial oxidative phosphorylation capacity in epicardial adipose tissue is associated with decreased concentration of adiponectin and severity of coronary atherosclerosis
    Authors: T Nakajima, T Yokota, Y Shingu, A Yamada, Y Iba, K Ujihira, S Wakasa, T Ooka, S Takada, R Shirakawa, T Katayama, T Furihata, A Fukushima, R Matsuoka, H Nishihara, F Dela, K Nakanishi, Y Matsui, S Kinugawa
    Sci Rep, 2019;9(1):3535.
    Species: Human
    Sample Types: Tissue Homogenates
  4. The effect of resistance exercise upon age-related systemic and local skeletal muscle inflammation
    Authors: AK Ziegler, SM Jensen, P Schjerling, AL Mackey, JL Andersen, M Kjaer
    Exp. Gerontol., 2019;121(0):19-32.
    Species: Human
    Sample Types: Serum
  5. Sex-specific effects of central adiposity and inflammatory markers on limbic microstructure
    Authors: C Metzler-Ba, JP Mole, E Leonaviciu, R Sims, EJ Kidd, B Ertefai, A Kelso-Mitc, F Gidney, F Fasano, J Evans, DK Jones, RJ Baddeley
    Neuroimage, 2019;189(0):793-803.
    Species: Human
    Sample Types: Plasma
  6. Sex difference in the mediation roles of an inflammatory factor (hsCRP) and adipokines on the relationship between adiposity and blood pressure
    Authors: YD Yang, CJ Zheng, YH Dong, ZY Zou, Y Lv, ZH Wang, ZG Yang, S Wang, B Dong, J Ma
    Hypertens. Res., 2019;0(0):.
    Species: Human
    Sample Types: Serum
  7. Dyslipidemia, subclinical inflammation, hepatic cholestasis and endothelial dysfunction in schoolchildren with excess fat: A study from the United Arab Emirates
    Authors: EH Aburawi, S Al Hamad, J Yasin, LA Almekhaini, AK Souid
    PLoS ONE, 2019;14(1):e0210316.
    Species: Human
    Sample Types: Blood
  8. Inter-ictal assay of peripheral circulating inflammatory mediators in migraine patients under adjunctive cervical non-invasive vagus nerve stimulation (nVNS): A proof-of-concept study
    Authors: SR Chaudhry, IS Lendvai, S Muhammad, P Westhofen, J Kruppenbac, L Scheef, H Boecker, D Scheele, R Hurlemann, TM Kinfe
    Brain Stimul, 2019;0(0):.
    Species: Human
    Sample Types: Serum
  9. Developmental trajectories of body mass index from childhood into late adolescence and subsequent late adolescence-young adulthood cardiometabolic risk markers
    Authors: K Oluwagbemi, AE Buyken, U Alexy, M Schmid, C Herder, U Nöthlings
    Cardiovasc Diabetol, 2019;18(1):9.
    Species: Human
    Sample Types: Plasma
  10. Association of serum concentrations of irisin and the adipokines adiponectin and leptin with epicardial fat in cardiovascular surgery patients
    Authors: H Kaneda, T Nakajima, A Haruyama, I Shibasaki, T Hasegawa, T Sawaguchi, T Kuwata, S Obi, T Arikawa, M Sakuma, H Amano, S Toyoda, H Fukuda, T Inoue
    PLoS ONE, 2018;13(8):e0201499.
    Species: Human
    Sample Types: Serum
  11. Plasma leptin, but not resistin, TNF-? and adiponectin, is associated with echocardiographic parameters of cardiac remodeling in patients with coronary artery disease
    Authors: AD Farca?, A Rusu, MA Stoia, LA Vida-Simit
    Cytokine, 2018;103(0):46-49.
    Species: Human
    Sample Types: Plasma
  12. Effect of adiposity on tissue-specific adiponectin secretion
    Authors: J Reneau, M Goldblatt, J Gould, T Kindel, A Kastenmeie, R Higgins, LR Rengel, K Schoyer, R James, B Obi, A Moosreiner, K Nicholson, D Sahoo, S Kidambi
    PLoS ONE, 2018;13(6):e0198889.
    Species: Human
    Sample Types: Serum
  13. Assessing C reactive protein/albumin ratio as a new biomarker for polycystic ovary syndrome: a case-control study of women from Bahraini medical clinics
    Authors: S Kalyan, A Goshtesabi, S Sarray, A Joannou, WY Almawi
    BMJ Open, 2018;8(10):e021860.
    Species: Human
    Sample Types: Serum
  14. Association between interleukin-6, C-reactive protein and adiponectin with adiposity: Findings from the 1993 pelotas (Brazil) birth cohort at 18 and 22?years
    Authors: AMB Menezes, PD Oliveira, FC Wehrmeiste, H Gonçalves, MCF Assunção, L Tovo-Rodri, GD Ferreira, IO Oliveira
    Cytokine, 2018;110(0):44-51.
    Species: Human
    Sample Types: Serum
  15. Are cytokines (IL-6, CRP and adiponectin) associated with bone mineral density in a young adult birth cohort?
    Authors: AMB Menezes, PD Oliveira, H Gonçalves, IO Oliveira, MCF Assunção, L Tovo-Rodri, GD Ferreira, FC Wehrmeiste
    BMC Musculoskelet Disord, 2018;19(1):427.
    Species: Human
    Sample Types: Blood
  16. Raet1e Polymorphisms Are Associated with Increased Risk of Developing Premature Coronary Artery Disease and with Some Cardiometabolic Parameters: The GEA Mexican Study
    Authors: R Posadas-Sá, B Roque-Ramí, JM Rodríguez-, N Pérez-Hern, JM Fragoso, T Villarreal, R Coral-Vázq, ME Tejero-Bar, C Posadas-Ro, G Vargas-Ala
    Mediators Inflamm., 2018;2018(0):1847696.
    Species: Human
    Sample Types: Serum
  17. Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA
    Authors: NS Harasymowi, ND Clement, A Azfer, R Burnett, DM Salter, AH Simpson
    Arthritis & rheumatology (Hoboken, N.J.), 2017;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  18. Ghrelin and hormonal markers under exercise training in patients with heart failure with preserved ejection fraction: results from the Ex-DHF pilot study
    Authors: TD Trippel, V Holzendorf, M Halle, G Gelbrich, K Nolte, A Duvinage, S Schwarz, T Rutscher, J Wiora, R Wachter, C Herrmann-L, HD Duengen, G Hasenfu beta, B Pieske, F Edelmann
    ESC Heart Fail, 2017;4(1):56-65.
    Species: Human
    Sample Types: Serum
  19. Metabolic milieu associates with impaired skeletal characteristics in obesity
    Authors: HT Viljakaine, HA Koistinen, T Tervaharti, T Sorsa, S Andersson, O Mäkitie
    PLoS ONE, 2017;12(6):e0179660.
    Species: Human
    Sample Types: Serum
  20. Myostatin and adipokines: The role of the metabolically unhealthy obese phenotype in muscle function and aerobic capacity in young adults
    Authors: LP Carvalho, RP Basso-Vane, L Di Thommaz, RG Mendes, MC Oliveira-J, RP Vieira, JC Bonjorno-J, CR Oliveira, R Luporini, A Borghi-Sil
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  21. Key Clinical Factors Predicting Adipokine and Oxidative Stress Marker Concentrations among Normal, Overweight and Obese Pregnant Women Using Artificial Neural Networks
    Authors: M Solis-Pare, G Estrada-Gu, O Perichart-, A Montoya-Es, M Guzmán-Hue, H Borboa-Oli, E Bravo-Flor, A Cardona-Pé, V Zaga-Clave, E Garcia-Lat, G Gonzalez-P, JA Hernández-, C Irles
    Int J Mol Sci, 2017;19(1):.
    Species: Human
    Sample Types: Serum
  22. The short-term and long-term effects of bariatric/metabolic surgery on subcutaneous adipose tissue inflammation in humans
    Authors: DK Hagman, I Larson, JN Kuzma, G Cromer, K Makar, KB Rubinow, KE Foster-Sch, B van Yserlo, PS Billing, RW Landerholm, M Crouthamel, DR Flum, DE Cummings, M Kratz
    Metab. Clin. Exp., 2017;70(0):12-22.
    Species: Human
    Sample Types: Plasma
  23. Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial
    Authors: TH Kim, JS Chang, KS Park, J Park, N Kim, JI Lee, ID Kong
    PLoS ONE, 2017;12(2):e0171771.
    Species: Human
    Sample Types: Serum
  24. The impact of obesity and adiponectin signaling in patients with renal cell carcinoma: A potential mechanism for the obesity paradox
    Authors: R Ito, S Narita, M Huang, T Nara, K Numakura, K Takayama, H Tsuruta, A Maeno, M Saito, T Inoue, N Tsuchiya, S Satoh, T Habuchi
    PLoS ONE, 2017;12(2):e0171615.
    Species: Human
    Sample Types: Serum
  25. Carbohydrates from Sources with a Higher Glycemic Index during Adolescence: Is Evening Rather than Morning Intake Relevant for Risk Markers of Type 2 Diabetes in Young Adulthood?
    Authors: T Diederichs, C Herder, S Ro beta bach, M Roden, SA Wudy, U Nöthlings, U Alexy, AE Buyken
    Nutrients, 2017;9(6):.
    Species: Human
    Sample Types: Plasma
  26. Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
    Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
    Oxid Med Cell Longev, 2017;2017(0):3937842.
    Species: Human
    Sample Types: Plasma
  27. The effect of BMI, serum leptin, and adiponectin levels on prognosis in patients with non-ischaemic dilated cardiomyopathy
    Authors: C Wojciechow, W Jache?, E Romuk, E Nowalany-K, A Tomasik, L Siemi?ska
    Endokrynol Pol, 2017;68(1):26-34.
    Species: Human
    Sample Types: Serum
  28. Association of Adiponectin with Subclinical Atherosclerosis in a Mexican-Mestizo Population
    Authors: JG Juárez-Roj, R Posadas-Sá, MDR Martínez-A, M Torres-Tam, E Jorge-Gala, EY Mancilla-V, C Posadas-Ro, GC Cardoso-Sa, MDC González-S, G Vargas-Ala, AX Medina-Urr
    Arch. Med. Res., 2017;48(1):73-78.
    Species: Human
    Sample Types: Serum
  29. Adipokines, C-reactive protein and Amyotrophic Lateral Sclerosis - results from a population- based ALS registry in Germany
    Authors: G Nagel, RS Peter, A Rosenbohm, W Koenig, L Dupuis, D Rothenbach, AC Ludolph
    Sci Rep, 2017;7(1):4374.
    Species: Human
    Sample Types: Serum
  30. Circulating adipokines in children with nonalcoholic fatty liver disease: possible noninvasive diagnostic markers
    Authors: AA Mohamed, S Sabry, AM Abdallah, NAA Elazeem, D Refaey, HAF Algebaly, GAE Fath, H Omar
    Ann Gastroenterol, 2017;30(4):457-463.
    Species: Human
    Sample Types: Serum
  31. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
    Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
    J Immunol Res, 2017;2017(0):6468097.
    Species: Human
    Sample Types: Plasma
  32. The effects of basal insulin peglispro vs. insulin glargine on lipoprotein particles by NMR and liver fat content by MRI in patients with diabetes
    Authors: TJ Orchard, B Cariou, MA Connelly, JD Otvos, S Zhang, CJ Antalis, T Ivanyi, BJ Hoogwerf
    Cardiovasc Diabetol, 2017;16(1):73.
    Species: Human
    Sample Types: Serum
  33. Serum levels of leptin and adiponectin and clinical parameters in women with fibromyalgia and overweight/obesity
    Authors: ES Paiva, A Andretta, ED Batista, MM Lobo, RC Miranda, R Nisihara, ME Schieferde, CL Boguszewsk
    Arch Endocrinol Metab, 2017;0(0):0.
    Species: Human
    Sample Types: Serum
  34. Altered Systemic Adipokines in Patients with Chronic Urticaria
    Authors: Hoang Kim Tu Trinh
    Int. Arch. Allergy Immunol, 2016;171(2):102-110.
    Species: Human
    Sample Types: Serum
  35. A rapid and precise method for measuring plasma apoE-rich HDL using polyethylene glycol and cation-exchange chromatography: a pilot study on the clinical significance of apoE-rich HDL measurements
    Authors: Toru Ikeda
    Clin. Chim. Acta, 2016;465(0):112-118.
    Species: Human
    Sample Types: Plasma
  36. Subclinical Inflammation and Endothelial Dysfunction in Young Patients with Diabetes: A Study from United Arab Emirates
    PLoS ONE, 2016;11(7):e0159808.
    Species: Human
    Sample Types: Serum
  37. Metabolic Syndrome and Selective Inflammatory Markers in Psoriatic Patients
    Authors: S Vachatova, C Andrys, J Krejsek, M Salavec, K Ettler, V Rehacek, E Cermakova, A Malkova, Z Fiala, L Borska
    J Immunol Res, 2016;2016(0):5380792.
    Species: Human
    Sample Types: Serum
  38. Effects of different degrees of insulin resistance on endothelial function in�obese�adults undergoing alternate day�fasting
    Authors: Kristin K Hoddy
    Nutr Healthy Aging, 2016;4(1):63-71.
    Species: Human
    Sample Types: Plasma
  39. Receptor-interacting protein 2 (RIP2) gene polymorphisms are associated with increased risk of subclinical atherosclerosis and clinical and metabolic parameters. The Genetics of Atherosclerotic Disease (GEA) Mexican study
    Authors: Rosalinda Posadas-Sá
    Exp. Mol. Pathol, 2016;102(1):1-6.
    Species: Human
    Sample Types: Serum
  40. Rice Bran Extract Reduces the Risk of Atherosclerosis in Post-Menopausal Vietnamese Women
    Authors: Bui Thi Nhung
    J. Nutr. Sci. Vitaminol, 2016;62(5):295-302.
    Species: Human
    Sample Types: Serum
  41. Insulin Resistance in Adipose Tissue but Not in Liver Is Associated with Aortic Valve Calcification
    Authors: E Jorge-Gala, C Posadas-Ro, M Torres-Tam, AX Medina-Urr, MA Rodas-D¡az, R Posadas-S , G Vargas-Ala, MD Gonz lez-S, GC Cardoso-Sa, JG Ju rez-Roj
    Dis. Markers, 2016;2016(0):9085474.
    Species: Human
    Sample Types: Serum
  42. Dietary patterns and cardiometabolic and endocrine plasma biomarkers in US women
    Authors: Hala B AlEssa
    Am. J. Clin. Nutr, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  43. comparative study of glucose homeostasis, lipids and lipoproteins, HDL functionality, and cardiometabolic parameters in modestly severely obese African Americans and White Americans with prediabetes: implications for the metabolic paradoxes.
    Authors: Healy S, Osei K, Gaillard T
    Diabetes Care, 2015;38(2):228-35.
    Species: Human
    Sample Types: Serum
  44. Adverse Fat Depots and Marrow Adiposity Are Associated With Skeletal Deficits and Insulin Resistance in Long-Term Survivors of Pediatric Hematopoietic Stem Cell Transplantation.
    Authors: Mostoufi-Moab S, Magland J, Isaacoff E, Sun W, Rajapakse C, Zemel B, Wehrli F, Shekdar K, Baker J, Long J, Leonard M
    J Bone Miner Res, 2015;30(9):1657-66.
    Species: Human
    Sample Types: Serum
  45. The effect of a Diabetes-Specific Cognitive Behavioral Treatment Program (DIAMOS) for patients with diabetes and subclinical depression: results of a randomized controlled trial.
    Authors: Hermanns N, Schmitt A, Gahr A, Herder C, Nowotny B, Roden M, Ohmann C, Kruse J, Haak T, Kulzer B
    Diabetes Care, 2015;38(4):551-60.
    Species: Human
    Sample Types: Serum
  46. Additive regulation of adiponectin expression by the mediterranean diet olive oil components oleic Acid and hydroxytyrosol in human adipocytes.
    Authors: Scoditti E, Massaro M, Carluccio M, Pellegrino M, Wabitsch M, Calabriso N, Storelli C, De Caterina R
    PLoS ONE, 2015;10(6):e0128218.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Longitudinal Analysis of Adiponectin through 20-Year Type 1 Diabetes Duration.
    Authors: LeCaire T, Palta M
    J Diabetes Res, 2015;2015(0):730407.
    Species: Human
    Sample Types: Plasma
  48. Role of adiponectin and free fatty acids on the association between abdominal visceral fat and insulin resistance.
    Authors: Medina-Urrutia A, Posadas-Romero C, Posadas-Sanchez R, Jorge-Galarza E, Villarreal-Molina T, Gonzalez-Salazar M, Cardoso-Saldana G, Vargas-Alarcon G, Torres-Tamayo M, Juarez-Rojas J
    Cardiovasc Diabetol, 2015;14(0):20.
    Species: Human
    Sample Types: Serum
  49. Ectopic fat storage in the pancreas using 1H-MRS: importance of diabetic status and modulation with bariatric surgery-induced weight loss.
    Authors: Gaborit B, Abdesselam I, Kober F, Jacquier A, Ronsin O, Emungania O, Lesavre N, Alessi M, Martin J, Bernard M, Dutour A
    Int J Obes (Lond), 2015;39(3):480-7.
    Species: Human
    Sample Types: Plasma
  50. Circulating adipokines and insulin resistance in subjects with combined cardiac and metabolic syndrome X.
    Authors: Liang K, Lee W, Lee W, Liao Y, Wang K, Lee I, Wang J, Sheu W
    Diabetol Metab Syndr, 2015;7(0):83.
    Species: Human
    Sample Types: Serum
  51. Phase I study of Efatutazone, an oral PPARgamma agonist, in patients with metastatic solid tumors.
    Authors: Murakami H, Ono A, Takahashi T, Onozawa Y, Tsushima T, Yamazaki K, Jikoh T, Boku N, Yamamoto N
    Anticancer Res, 2014;34(9):5133-41.
    Species: Human
    Sample Types: Plasma
  52. Association of serum vaspin and adiponectin levels with renal function in patients with or without type 2 diabetes mellitus.
    Authors: Yan, Meiyu, Su, Bin, Peng, Wenhui, Li, Liang, Li, Hailing, Zhuang, Jianhui, Lu, Yuyan, Jian, Weixia, Wei, Yidong, Li, Weiming, Qu, Shen, Xu, Yawei
    J Diabetes Res, 2014;2014(0):868732.
    Species: Human
    Sample Types: Plasma
  53. Effect of Sfrp5 on cytokine release and insulin action in primary human adipocytes and skeletal muscle cells.
    Authors: Carstensen M, Wiza C, Rohrig K, Fahlbusch P, Roden M, Herder C, Ouwens D
    PLoS ONE, 2014;9(1):e85906.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Adiponectin and bariatric surgery: associations with diabetes and cardiovascular disease in the Swedish Obese Subjects Study.
    Authors: Herder C, Peltonen M, Svensson P, Carstensen M, Jacobson P, Roden M, Sjostrom L, Carlsson L
    Diabetes Care, 2014;37(5):1401-9.
    Species: Human
    Sample Types: Serum
  55. Association between traffic-related air pollution, subclinical inflammation and impaired glucose metabolism: results from the SALIA study.
    Authors: Teichert T, Vossoughi M, Vierkotter A, Sugiri D, Schikowski T, Schulte T, Roden M, Luckhaus C, Herder C, Kramer U
    PLoS ONE, 2013;8(12):e83042.
    Species: Human
    Sample Types: Plasma
  56. Obesity and IL-6 interact in modulating the response to endotoxemia in mice.
    Cytokine, 2013;61(1):71-7.
    Species: Mouse
    Sample Types: Plasma
  57. Exenatide as a weight-loss therapy in extreme pediatric obesity: a randomized, controlled pilot study.
    Authors: Kelly AS, Metzig AM, Rudser KD, Fitch AK, Fox CK, Nathan BM, Deering MM, Schwartz BL, Abuzzahab MJ, Gandrud LM, Moran A, Billington CJ, Schwarzenberg SJ
    Obesity (Silver Spring), 2012;20(2):364-70.
    Species: Human
    Sample Types: Plasma
  58. A prospective study of circulating adipokine levels and risk of multiple myeloma.
    Authors: Hofmann J, Liao L, Pollak M, Wang Y, Pfeiffer R, Baris D, Andreotti G, Lan Q, Landgren O, Rothman N, Purdue M
    Blood, 2012;120(22):4418-20.
    Species: Human
    Sample Types: Plasma
  59. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
    Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
    Obesity (Silver Spring), 2011;19(11):2130-6.
    Species: Human
    Sample Types: Plasma
  60. Adipocytokines and CD34 progenitor cells in Alzheimer's disease.
    Authors: Bigalke B, Schreitmuller B, Sopova K, Paul A, Stransky E, Gawaz M, Stellos K, Laske C
    PLoS ONE, 2011;6(5):e20286.
    Species: Human
    Sample Types: Plasma
  61. Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks.
    Authors: Nanda S, Savvidou M, Syngelaki A, Akolekar R, Nicolaides KH
    Prenat. Diagn., 2011;31(2):135-41.
    Species: Human
    Sample Types: Serum
  62. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
    Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
    Int J Obes (Lond), 2011;35(6):800-9.
    Species: Human
    Sample Types: Serum
  63. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
    Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
    Br. J. Cancer, 2011;104(1):51-9.
    Species: Human
    Sample Types: Serum
  64. Exercise training improves sleep pattern and metabolic profile in elderly people in a time-dependent manner.
    Authors: Lira FS, Pimentel GD, Santos RV, Oyama LM, Damaso AR, Oller do Nascimento CM, Viana VA, Boscolo RA, Grassmann V, Santana MG, Esteves AM, Tufik S, de Mello MT
    Lipids Health Dis, 2011;10(0):113.
    Species: Human
    Sample Types: Plasma
  65. Serum adiponectin acutely after an ischemic stroke: implications for a long-lasting, suppressed anti-inflammatory role.
    Authors: Marousi S, Theodorou G, Karakantza M, Papathanasopoulos P, Ellul J
    Acta Neurol. Scand., 2010;121(4):277-84.
    Species: Human
    Sample Types: Serum
  66. An antiinflammatory dietary mix modulates inflammation and oxidative and metabolic stress in overweight men: a nutrigenomics approach.
    Authors: Bakker GC, van Erk MJ, Pellis L, Wopereis S, Rubingh CM, Cnubben NH, Kooistra T, van Ommen B, Hendriks HF
    Am. J. Clin. Nutr., 2010;91(4):1044-59.
    Species: Human
    Sample Types: Plasma
  67. Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition.
    Authors: Bunck MC, Diamant M, Eliasson B, Corner A, Shaginian RM, Heine RJ, Taskinen MR, Yki-Jarvinen H, Smith U
    Diabetes Care, 2010;33(8):1734-7.
    Species: Human
    Sample Types: Serum
  68. Nonalcoholic fatty liver disease as the transducer of hepatic oversecretion of very-low-density lipoprotein-apolipoprotein B-100 in obesity.
    Authors: Chan DC, Watts GF, Gan S
    Arterioscler. Thromb. Vasc. Biol., 2010;30(5):1043-50.
    Species: Human
    Sample Types: Plasma
  69. Are there socioeconomic inequalities in cardiovascular risk factors in childhood, and are they mediated by adiposity? Findings from a prospective cohort study.
    Authors: Howe LD, Galobardes B, Sattar N
    Int J Obes (Lond), 2010;34(7):1149-59.
    Species: Human
    Sample Types: Plasma
  70. Adiponectin and its association with bone mass accrual in childhood.
    Authors: Sayers A, Timpson NJ, Sattar N, Deanfield J, Hingorani AD, Davey-Smith G, Tobias JH
    J. Bone Miner. Res., 2010;25(10):2212-20.
    Species: Human
    Sample Types: Plasma
  71. First-trimester prediction of gestational diabetes mellitus: examining the potential of combining maternal characteristics and laboratory measures.
    Authors: Savvidou M, Nelson SM, Makgoba M, Messow CM, Sattar N, Nicolaides K
    Diabetes, 2010;59(12):3017-22.
    Species: Human
    Sample Types: Serum
  72. Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
    Authors: Furet JP, Kong LC, Tap J, Poitou C, Basdevant A, Bouillot JL, Mariat D, Corthier G, Dore J, Henegar C, Rizkalla S, Clement K
    Diabetes, 2010;59(12):3049-57.
    Species: Human
    Sample Types: Plasma
  73. Improvements in coronary heart disease risk indicators by alternate-day fasting involve adipose tissue modulations.
    Authors: Bhutani S, Klempel MC, Berger RA, Varady KA
    Obesity (Silver Spring), 2010;18(11):2152-9.
    Species: Human
    Sample Types: Plasma
  74. Regulation of the fibrosis and angiogenesis promoter SPARC/osteonectin in human adipose tissue by weight change, leptin, insulin, and glucose.
    Authors: Kos K, Wong S, Tan B, Gummesson A, Jernas M, Franck N, Kerrigan D, Nystrom FH, Carlsson LM, Randeva HS, Pinkney JH, Wilding JP
    Diabetes, 2009;58(8):1780-8.
    Species: Human
    Sample Types: Serum
  75. Association between the adiponectin promoter rs266729 gene variant and oxidative stress in patients with diabetes mellitus.
    Authors: Prior SL, Gable DR, Cooper JA, Bain SC, Hurel SJ, Humphries SE, Stephens JW
    Eur. Heart J., 2009;30(10):1263-9.
    Species: Human
    Sample Types: Plasma
  76. Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
    Authors: Troseid M, Seljeflot I, Hjerkinn EM, Arnesen H
    Diabetes Care, 2009;32(3):486-92.
    Species: Human
    Sample Types: Serum
  77. Serial changes in adiponectin and BNP in ACS patients: paradoxical associations with each other and with prognosis.
    Authors: Ang DS, Welsh P, Watt P, Nelson SM, Struthers A, Sattar N
    Clin. Sci., 2009;117(1):41-8.
    Species: Human
    Sample Types: Plasma
  78. Environmental tobacco smoke and cardiometabolic risk in young children: results from a survey in south-west Germany.
    Authors: Nagel G, Arnold FJ, Wilhelm M, Link B, Zoellner I, Koenig W
    Eur. Heart J., 2009;30(15):1885-93.
    Species: Human
    Sample Types: Plasma
  79. HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
    Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J,
    AIDS, 2009;23(8):929-39.
    Species: Human
    Sample Types: Plasma
  80. Recombinant luteinizing hormone induces increased production of ovarian follicular adiponectin in vivo: implications for enhanced insulin sensitivity.
    Authors: Gutman G, Barak V, Maslovitz S, Amit A, Lessing JB, Geva E
    Fertil. Steril., 2009;91(5):1837-41.
    Species: Human
    Sample Types: Follicular Fluid
  81. Resistin, adiponectin, and risk of heart failure the Framingham offspring study.
    Authors: Frankel DS, Vasan RS, D'Agostino RB, Benjamin EJ, Levy D, Wang TJ, Meigs JB
    J. Am. Coll. Cardiol., 2009;53(9):754-62.
    Species: Human
    Sample Types: Plasma
  82. Plasma 25-hydroxyvitamin d is associated with markers of the insulin resistant phenotype in nondiabetic adults.
    Authors: Liu E, Meigs JB, Pittas AG, McKeown NM, Economos CD, Booth SL, Jacques PF
    J. Nutr., 2009;139(2):329-34.
    Species: Human
    Sample Types: Plasma
  83. Adiponectin and functional adiponectin receptor 1 are expressed by airway epithelial cells in chronic obstructive pulmonary disease.
    Authors: Miller M, Cho JY, Pham A, Ramsdell J, Broide DH
    J. Immunol., 2009;182(1):684-91.
    Species: Human
    Sample Types: BALF
  84. Adiponectin plasma levels are increased by atorvastatin treatment in subjects at high cardiovascular risk.
    Authors: Blanco-Colio LM, Martin-Ventura JL, Gomez-Guerrero C, Masramon X, de Teresa E, Farsang C, Gaw A, Gensini G, Leiter LA, Langer A, Egido J
    Eur. J. Pharmacol., 2008;586(1):259-65.
    Species: Human
    Sample Types: Plasma
  85. Adiponectin gene variants are associated with insulin sensitivity in response to dietary fat consumption in Caucasian men.
    Authors: Perez-Martinez P, Lopez-Miranda J, Cruz-Teno C, Delgado-Lista J, Jimenez-Gomez Y, Fernandez JM, Gomez MJ, Marin C, Perez-Jimenez F, Ordovas JM
    J. Nutr., 2008;138(9):1609-14.
    Species: Human
    Sample Types: Plasma
  86. Circulating levels of adiponectin and leptin at 23-25 weeks of pregnancy in women with impaired placentation and in those with established fetal growth restriction.
    Authors: Savvidou MD, Sotiriadis A, Kaihura C, Nicolaides KH, Sattar N
    Clin. Sci., 2008;115(7):219-24.
    Species: Human
    Sample Types: Plasma
  87. Importance of the high-molecular-mass isoform of adiponectin in improved insulin sensitivity with rosiglitazone treatment in HIV disease.
    Authors: Qurashi S, Mynarcik DC, McNurlan MA, Ahn H, Ferris R, Gelato MC
    Clin. Sci., 2008;115(6):197-202.
    Species: Human
    Sample Types: Plasma
  88. Gene expression profiling of human skeletal muscle in response to stabilized weight loss.
    Authors: Larrouy D, Barbe P, Valle C, Dejean S, Pelloux V, Thalamas C, Bastard JP, Le Bouil A, Diquet B, Clement K, Langin D, Viguerie N
    Am. J. Clin. Nutr., 2008;88(1):125-32.
    Species: Human
    Sample Types: Plasma
  89. Association of IL-1ra and adiponectin with C-peptide and remission in patients with type 1 diabetes.
    Authors: Pfleger C, Mortensen HB, Hansen L, Herder C, Roep BO, Hoey H, Aanstoot HJ, Kocova M, Schloot NC
    Diabetes, 2008;57(4):929-37.
    Species: Human
    Sample Types: Serum
  90. Fish oil in combination with high or low intakes of linoleic acid lowers plasma triacylglycerols but does not affect other cardiovascular risk markers in healthy men.
    Authors: Damsgaard CT, Frokiaer H, Andersen AD, Lauritzen L
    J. Nutr., 2008;138(6):1061-6.
    Species: Human
    Sample Types: Plasma
  91. Retinol-binding protein 4 and lipocalin-2 in childhood and adolescent obesity: when children are not just "small adults".
    Authors: Kanaka-Gantenbein C, Margeli A, Pervanidou P, Sakka S, Mastorakos G, Chrousos GP, Papassotiriou I
    Clin. Chem., 2008;54(7):1176-82.
    Species: Human
    Sample Types: Serum
  92. Factors contributing to the risk of cardiovascular disease reflected by plasma adiponectin: data from the coronary risk factors for atherosclerosis in women (CORA) study.
    Authors: Zyriax BC, Algenstaedt P, Hess UF, Schoffauer M, Bamberger C, Boeing H, Windler E
    Atherosclerosis, 2008;200(2):403-9.
    Species: Human
    Sample Types: Plasma
  93. Circulating adiponectin levels and mortality in elderly men with and without cardiovascular disease and heart failure.
    Authors: Wannamethee SG, Whincup PH, Lennon L, Sattar N
    Arch. Intern. Med., 2007;167(14):1510-7.
    Species: Human
    Sample Types: Plasma
  94. Nitrosative stress predicts the presence and severity of nonalcoholic fatty liver at different stages of the development of insulin resistance and metabolic syndrome: possible role of vitamin A intake.
    Authors: Musso G, Gambino R, De Michieli F, Biroli G, Premoli A, Pagano G, Bo S, Durazzo M, Cassader M
    Am. J. Clin. Nutr., 2007;86(3):661-71.
    Species: Human
    Sample Types: Serum
  95. Intake of phenol-rich virgin olive oil improves the postprandial prothrombotic profile in hypercholesterolemic patients.
    Authors: Ruano J, Lopez-Miranda J, de la Torre R, Delgado-Lista J, Fernandez J, Caballero J, Covas MI, Jimenez Y, Perez-Martinez P, Marin C, Fuentes F, Perez-Jimenez F
    Am. J. Clin. Nutr., 2007;86(2):341-6.
    Species: Human
    Sample Types: Plasma
  96. Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
    Authors: Farese RV, Sajan MP, Yang H, Li P, Mastorides S, Gower WR, Nimal S, Choi CS, Kim S, Shulman GI, Kahn CR, Braun U, Leitges M
    J. Clin. Invest., 2007;117(8):2289-301.
    Species: Human
    Sample Types: Serum
  97. Adiponectin and leptin in maternal serum, cord blood, and breast milk.
    Authors: Weyermann M, Beermann C, Brenner H, Rothenbacher D
    Clin. Chem., 2006;52(11):2095-102.
    Species: Human
    Sample Types: Serum
  98. Altered hepatic expression of SREBP-1 and PPARgamma is associated with liver injury in insulin-resistant lipodystrophic HIV-infected patients.
    Authors: Lemoine M, Barbu V, Girard PM, Kim M, Bastard JP, Wendum D, Paye F, Housset C, Capeau J, Serfaty L
    AIDS, 2006;20(3):387-95.
    Species: Human
    Sample Types: Serum
  99. Non-esterified fatty acid concentrations are independently associated with hepatic steatosis in obese subjects.
    Authors: Holt HB, Wild SH, Wood PJ, Zhang J, Darekar AA, Dewbury K, Poole RB, Holt RI, Phillips DI, Byrne CD
    Diabetologia, 2006;49(1):141-8.
    Species: Human
    Sample Types: Serum
  100. Relation of improvement in endothelium-dependent flow-mediated vasodilation after rosiglitazone to changes in asymmetric dimethylarginine, endothelin-1, and C-reactive protein in nondiabetic patients with the metabolic syndrome.
    Authors: Wang TD, Chen WJ, Cheng WC, Lin JW, Chen MF, Lee YT
    Am. J. Cardiol., 2006;98(8):1057-62.
    Species: Human
    Sample Types: Plasma
  101. Additive beneficial effects of fenofibrate combined with candesartan in the treatment of hypertriglyceridemic hypertensive patients.
    Authors: Koh KK, Quon MJ, Han SH, Chung WJ, Ahn JY, Kim JA, Lee Y, Shin EK
    Diabetes Care, 2006;29(2):195-201.
    Species: Human
    Sample Types: Plasma
  102. Adiponectin and other adipocytokines as predictors of markers of triglyceride-rich lipoprotein metabolism.
    Authors: Chan DC, Watts GF, Ng TW, Uchida Y, Sakai N, Yamashita S, Barrett PH
    Clin. Chem., 2005;51(3):578-85.
    Species: Human
    Sample Types: Plasma
  103. Tumor necrosis factor alpha and adiponectin in bone marrow interstitial fluid from patients with acute myeloid leukemia inhibit normal hematopoiesis.
    Authors: Iversen PO, Wiig H
    Clin. Cancer Res., 2005;11(19):6793-9.
    Species: Human
    Sample Types: Bone Marrow Fluid

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Human Total Adiponectin/Acrp30 Quantikine ELISA Kit
By Anonymous on 11/13/2018
Sample Tested: Serum

Data looks good!


Human Total Adiponectin/Acrp30 Quantikine ELISA Kit
By Anonymous on 04/26/2018
Sample Tested: Pancreatic cancer cells

Human Total Adiponectin/Acrp30 Quantikine ELISA Kit
By Anonymous on 10/23/2017
Sample Tested: Serum