Human TRAF-4 Antibody

Catalog # Availability Size / Price Qty
MAB3279
MAB3279-SP
Detection of Human TRAF‑4 by Western Blot.
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Product Details
Citations (1)
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Human TRAF-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human TRAF-4. In Western blots, this antibody does not cross-react with rhTRAF-1, -2, -3, -5, or -6 in Western blots.
Source
Monoclonal Rat IgG2A Clone # 433709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TRAF-4
Met1-Ser470
Accession # Q9BUZ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRAF‑4 antibody by Western Blot. View Larger

Detection of Human TRAF‑4 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2.5 µg/mL of Human TRAF-4 Monoclonal Antibody (Catalog # MAB3279) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAF-4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-4

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Seven members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-4, also known as CART1 and MLN62, is a 470 amino acid, 53 kDa protein. A second isoform has been identified and lacks amino acids 157‑428. TRAF-4 is unique member of the TRAF family that localizes to the nucleus and does not regulate signaling through cell surface receptors. TRAF-4 is highly expressed in human breast carcinomas and during embryogenisis.

Long Name
TNF Receptor-Associated Factor 4
Entrez Gene IDs
9618 (Human); 22032 (Mouse)
Alternate Names
CART1; CART1RING finger protein 83; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; MLN62; MLN62cysteine-rich domain associated with ring and TRAF domain; RNF83malignant 62; TNF receptor-associated factor 4; TRAF4; TRAF-4; tumor necrosis receptor-associated factor 4A

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Citation for Human TRAF-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2
    Authors: J Li, P Wang, Z Xie, S Wang, S Cen, M Li, W Liu, S Tang, G Ye, G Zheng, H Su, M Ma, X Wu, Y Wu, H Shen
    Cell Death Differ., 2019-05-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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