Human TRAIL/TNFSF10 Antibody

(10 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human TRAIL/TNFSF10 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 75411
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10
    Thr95-Gly281
    Accession # P50591
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human brain (occipital cortex)
  • Neutralization
    Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 2-8 ng/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Human TRAIL/TNFSF10 Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB375). The ND50 is typically 2-8 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL/TNFSF10
TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.
  • Long Name:
    TNF-related Apoptosis-inducing Ligand
  • Entrez Gene IDs:
    8743 (Human); 22035 (Mouse)
  • Alternate Names:
    Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Combination of IAP antagonist and IFN? activates novel caspase-10- and RIPK1-dependent cell death pathways
    Authors: MC Tanzer, N Khan, JA Rickard, N Etemadi, N Lalaoui, SK Spall, JM Hildebrand, D Segal, M Miasari, D Chau, WL Wong, M McKinlay, SK Chunduru, CA Benetatos, SM Condon, JE Vince, MJ Herold, J Silke
    Cell Death Differ, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Negative control of TRAIL-R1 signaling by transforming growth factor ?1 in pancreatic tumor cells involves Smad-dependent down regulation of TRAIL-R1
    Cell Signal, 2016;28(11):1652-1662.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Human plasmacytoid dendritic cells are equipped with antigen-presenting and tumoricidal capacities.
    Authors: Tel J, Smits E, Anguille S, Joshi R, Figdor C, de Vries I
    Blood, 2012;120(19):3936-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity.
    Authors: Kalb ML, Glaser A, Stary G, Koszik F, Stingl G
    J. Immunol., 2012;188(4):1583-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. TRAIL stimulates proliferation of vascular smooth muscle cells via activation of NF-kappaB and induction of insulin-like growth factor-1 receptor.
    Authors: Kavurma MM, Schoppet M, Bobryshev YV, Khachigian LM, Bennett MR
    J. Biol. Chem., 2008;283(12):7754-62.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN.
    Authors: Inglefield JR, Dumitru CD, Alkan SS, Gibson SJ, Lipson KE, Tomai MA, Larson CJ, Vasilakos JP
    J. Interferon Cytokine Res., 2008;28(4):253-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells.
    Authors: Krysov SV, Krysov&lt;/LastName&gt;&lt;ForeNam SV&lt;/Initia, Rowley TF, Al-Shamkhani A
    Mol. Cancer Ther., 2007;6(2):703-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation.
    Authors: Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK
    Cell Death Differ., 2006;13(10):1789-801.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104.
    Authors: Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D
    J. Leukoc. Biol., 2005;78(2):359-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Adenoviral gene transfer of tumor necrosis factor-related apoptosis-inducing ligand overcomes an impaired response of hepatoma cells but causes severe apoptosis in primary human hepatocytes.
    Authors: Armeanu S, Lauer UM, Smirnow I, Schenk M, Weiss TS, Gregor M, Bitzer M
    Cancer Res., 2003;63(10):2369-72.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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