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Ancillary Products Available
Human TRANCE / TNFSF11 / RANK L ELISA Standard Curve
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Product Details
Citations (22)
Supplemental Products
Reviews (1)


Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human TRANCE / TNFSF11 / RANK L. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human TRANCE / TNFSF11 / RANK L ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.


TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.

Long Name:
TNF-related Activation-induced Cytokine
Entrez Gene IDs:
8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names:
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

Citations for Human TRANCE/RANK L/TNFSF11 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. RANKL Is Independently Associated with Increased Risks of Non-Alcoholic Fatty Liver Disease in Chinese Women with PCOS: A Cross-Sectional Study
    Authors: N Lu, C Shan, JR Fu, Y Zhang, YY Wang, YC Zhu, J Yu, J Cai, SX Li, T Tao, W Liu
    Journal of Clinical Medicine, 2023;12(2):.
    Species: Human
    Sample Types: Serum
  2. RANKL neutralisation prevents osteoclast activation in a human in vitro ameloblastoma-bone model
    Authors: J Pape, D Bakkalci, RA Hosni, BS Simpson, K Heikinheim, S Fedele, U Cheema
    Journal of tissue engineering, 2022;13(0):2041731422114.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Hypoxia Preconditioned Serum (HPS) Promotes Osteoblast Proliferation, Migration and Matrix Deposition
    Authors: J Jiang, L Röper, S Alageel, U Dornseifer, AF Schilling, E Hadjipanay, HG Machens, P Moog
    Oncogene, 2022;10(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. e-Cigarette Vapour Condensate Reduces Viability and Impairs Function of Human Osteoblasts, in Part, via a Nicotine Dependent Mechanism
    Authors: T Nicholson, L Davis, ET Davis, M Newton Ede, A Scott, SW Jones
    Toxics, 2022;10(9):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Rank-Rankl-Opg Axis in Multiple Sclerosis: The Contribution of Placenta
    Authors: S Passaponti, L Ermini, G Acconci, FM Severi, R Romagnoli, S Cutrupi, M Clerico, G Guerrera, F Ietta
    Cells, 2022;11(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Regulation of Cellular Senescence Is Independent from Profibrotic Fibroblast-Deposited ECM
    Authors: KEC Blokland, H Habibie, T Borghuis, GJ Teitsma, M Schuliga, BN Melgert, DA Knight, CA Brandsma, SD Pouwels, JK Burgess
    Cells, 2021;10(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Peri-implant parameters and bone metabolic markers among water-pipe users treated with photodynamic therapy
    Authors: N Al-Hamoudi, Y Alali, K Al-Aali, AA Alhumaidan, E Heer, SA Tanveer, M Naseem, F Vohra, T Abduljabba
    Photodiagnosis and photodynamic therapy, 2021;37(0):102655.
    Species: Human
    Sample Types: Sulcular Fluid
  8. Role of PDT as an adjunct to SRP on whole salivary RANKL and OPG ratio in type-2 diabetic and normoglycemic individuals with chronic periodontitis
    Authors: B Shetty, DD Divakar, AA Al-Kheraif, AO Alharbi, MST Almutairi, MM Alanazi
    Photodiagnosis and photodynamic therapy, 2021;0(0):102220.
    Species: Human
    Sample Types: Saliva
  9. T helper cells in synovial fluid of patients with rheumatoid arthritis primarily have a Th1 and a CXCR3+Th2 phenotype
    Authors: J Aldridge, AH Ekwall, L Mark, B Bergström, K Andersson, I Gjertsson, AC Lundell, A Rudin
    Arthritis Res Ther, 2020;22(1):245.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Clinical Response to the CD95-Ligand Inhibitor Asunercept Is Defined by a Pro-Inflammatory Serum Cytokine Profile
    Authors: A Radujkovic, T Boch, F Nolte, D Nowak, C Kunz, A Gieffers, C Müller-Tid, P Dreger, WK Hofmann, T Luft
    Cancers, 2020;12(12):.
    Species: Human
    Sample Types: Serum
  11. Sex Differences in Proatherogenic Cytokine Levels
    Authors: S Bernardi, B Toffoli, F Tonon, M Francica, E Campagnolo, T Ferretti, S Comar, F Giudici, E Stenner, B Fabris
    Int J Mol Sci, 2020;21(11):.
    Species: Human
    Sample Types:
  12. 6-Shogaol Suppresses 2-Amino-1-Methyl-6-Phenylimidazo [4,5-b] Pyridine (PhIP)-Induced Human 786-O Renal Cell Carcinoma Osteoclastogenic Activity and Metastatic Potential
    Authors: IJ Yeh, SC Chen, MC Yen, YH Wu, CH Hung, PL Kuo
    Nutrients, 2019;11(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. The effect of compressive force combined with mechanical vibration on human alveolar bone osteoblasts
    Authors: C Chatmahamo, A Pravithara, S Suttapreya, C Leethanaku
    J Oral Biol Craniofac Res, 2019;9(1):81-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cells in vitro
    Authors: S Benjakul, C Leethanaku, S Jitpukdeeb
    J Oral Biol Craniofac Res, 2019;9(3):251-255.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. RANKL Inhibits the Production of Osteoprotegerin from Smooth Muscle Cells under Basal Conditions and following Exposure to Cyclic Strain
    Authors: C Davenport, E Harper, KD Rochfort, H Forde, D Smith, PM Cummins
    J. Vasc. Res., 2018;55(2):111-123.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Gender-independent efficacy of mesenchymal stem cell therapy in sex hormone-deficient bone loss via immunosuppression and resident stem cell recovery
    Authors: BD Sui, J Chen, XY Zhang, T He, P Zhao, CX Zheng, M Li, CH Hu, Y Jin
    Exp. Mol. Med., 2018;50(12):166.
    Species: Human
    Sample Types: Serum
  17. Periodontal disease influences osteoclastogenic bone markers in subjects with and without rheumatoid arthritis
    Authors: J Panezai, A Ghaffar, M Altamash, PE Engström, A Larsson
    PLoS ONE, 2018;13(6):e0197235.
    Species: Human
    Sample Types: Serum
  18. Association of salivary RANKL and osteoprotegerin levels with periodontal health.
    Authors: A A Ochanji, N K Matu, T K Mulli
    Clinical and Experimental Dental Research, 2017;0(0):2057-4347.
    Species: Human
    Sample Types: Saliva
  19. Cancer-associated fibroblasts promote bone invasion in oral squamous cell carcinoma
    Authors: AA Elmusrati, AE Pilborough, SA Khurram, DW Lambert
    Br. J. Cancer, 2017;117(6):867-875.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. RUNX2 Mutation Impairs 1?,25-Dihydroxyvitamin D3 mediated Osteoclastogenesis in Dental Follicle Cells
    Authors: XZ Wang, XY Sun, CY Zhang, X Yang, WJ Yan, LH Ge, SG Zheng
    Sci Rep, 2016;6(0):24225.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Effect of low-level laser therapy on bisphosphonate-treated osteoblasts
    Authors: Sang-Hun Shin
    Maxillofac Plast Reconstr Surg, 2016;38(1):48.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Cooperation between IL-7 Receptor and Integrin alpha2beta1 (CD49b) Drives Th17-Mediated Bone Loss.
    Authors: El Azreq M, Arseneault C, Boisvert M, Page N, Allaeys I, Poubelle P, Tessier P, Aoudjit F
    J Immunol, 2015;195(9):4198-209.
    Species: Human
    Sample Types: Cell Culture Supernates


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By Anonymous on 08/11/2020
Sample Tested: Cell Culture Media,Serum