Detection of Human Transglutaminase 7/TGM7 by Western Blot.
Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Human Transglutaminase 7/TGM7 Monoclonal Antibody (Catalog # MAB5426) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Transglutaminase 7/TGM7 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transglutaminase 7/TGM7
Transglutaminase 7 (TGM7), also known as protein-glutamine gamma -glutamyltransferase Z, TGase Z and TGZ, is an 80 kDa, widely expressed member of the Transglutaminase superfamily and family. Human Transglutaminase 7 is 710 amino acids (aa) in length and contains a Transglutaminase family, C-terminal Ig like domain (aa 608‑705). Human Transglutaminase 7 shares 78% aa identity with mouse Transglutaminase 7. Like other Transglutaminases, Transglutaminase 7 is an enzyme that stabilizes protein assembly through the formation of intra- or intermolecular N epsilon ( gamma -glutamyl)lysine bonds. It catalyzes a calcium-dependent transferase reaction between the gamma -carboxamide group of a peptide-bound glutamine residue and various primary amines, most commonly the epsilon -amino group of lysine residues.
Have you used Human Transglutaminase 7/TGM7 Antibody?
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