Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Transglutaminase 7/TGM7 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 7/TGM7

Specificity

Detects human Transglutaminase 7/TGM7 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Transglutaminase 3 is observed, and less than 2% cross-reactivity with rhTransglutaminase 2, rhTransglutaminase 4, and recombinant mouse Transglutaminase 2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Transglutaminase 7/TGM7 Antibody

Detection of Human Transglutaminase 7/TGM7 antibody by Western Blot.

Detection of Human Transglutaminase 7/TGM7 by Western Blot.

Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Transglutaminase 7/TGM7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 7/TGM7 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Transglutaminase 7/TGM7 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human thyroid tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Transglutaminase 7/TGM7

Transglutaminase 7 (TG7), encoded by the TGM7 gene, is also known as protein-glutamine-g-glutamyltransferase Z (Tgase Z) (1). It belongs to the family of Transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (2‑4). TG7 is ubiquitously expressed in humans (1). Members of the TGM family have been implicated in a variety of human diseases including neurodegenerative diseases, celiac disease, lamellar ichthyosis, bleeding disorders, cataract formation, atherosclerosis, and others (5).

References

  1. Grenard, P. et al. (2001) J. Biol. Chem. 276:33066.
  2. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  3. Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
  4. Griffin, M. et al. (2002) Biochem. J. 368:377.
  5. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.

Alternate Names

TGM7, TGMZ, TGZ

Entrez Gene IDs

116179 (Human); 640543 (Mouse); 691932 (Rat)

Gene Symbol

TGM7

Additional Transglutaminase 7/TGM7 Products

Product Documents for Human Transglutaminase 7/TGM7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Transglutaminase 7/TGM7 Antibody

For research use only

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