Human Transglutaminase 7/TGM7 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Transglutaminase 7/TGM7 by Western Blot.
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Product Details
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Human Transglutaminase 7/TGM7 Antibody Summary

Species Reactivity
Detects human Transglutaminase 7/TGM7 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Transglutaminase 3 is observed, and less than 2% cross-reactivity with rhTransglutaminase 2, rhTransglutaminase 4, and recombinant mouse Transglutaminase 2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 7/TGM7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Transglutaminase 7/TGM7 antibody by Western Blot. View Larger

Detection of Human Transglutaminase 7/TGM7 by Western Blot. Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Transglutaminase 7/TGM7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 7/TGM7 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 7/TGM7

Transglutaminase 7 (TG7), encoded by the TGM7 gene, is also known as protein-glutamine-g-glutamyltransferase Z (Tgase Z) (1). It belongs to the family of Transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (2‑4). TG7 is ubiquitously expressed in humans (1). Members of the TGM family have been implicated in a variety of human diseases including neurodegenerative diseases, celiac disease, lamellar ichthyosis, bleeding disorders, cataract formation, atherosclerosis, and others (5).

  1. Grenard, P. et al. (2001) J. Biol. Chem. 276:33066.
  2. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  3. Chen, J.S.K. and Mehta K. (1999) Internat. J. Biochem. Cell Biol. 31:817.
  4. Griffin, M. et al. (2002) Biochem. J. 368:377.
  5. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
Entrez Gene IDs
116179 (Human); 640543 (Mouse); 691932 (Rat)
Alternate Names
protein-glutamine gamma-glutamyltransferase Z; TG(Z); TGase Z; TGase-7; TGM7; TGMZ; TGMZEC; TGZ; Transglutaminase 7; Transglutaminase Z; transglutaminase-7

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