Detects human Transglutaminase 7/TGM7 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Transglutaminase 3 is observed, and less than 2% cross-reactivity with rhTransglutaminase 2, rhTransglutaminase 4, and recombinant mouse Transglutaminase 2 is observed.
Polyclonal Sheep IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 7/TGM7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Transglutaminase 7/TGM7 by Western Blot.
Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Transglutaminase 7/TGM7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5426) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Transglutaminase 7/TGM7 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transglutaminase 7/TGM7
Transglutaminase 7 (TG7), encoded by the TGM7 gene, is also known as protein-glutamine-g-glutamyltransferase Z (Tgase Z). It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions. TG7 is ubiquitously expressed in humans. Members of the TGM family have been implicated in a variety of human diseases including neurodegenerative diseases, celiac disease, lamellar ichthyosis, bleeding disorders, cataract formation, atherosclerosis, and others.
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