Human Trypsin 1/PRSS1 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Human Trypsin 1/PRSS1 Antibody Summary

Species Reactivity
Detects human Trpysin 1/PRSS1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) Trypsin 3, recombinant human (rh) Trypsin 2, or rhTrypsin 3.
Monoclonal Mouse IgG1 Clone # 446628
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Trypsin 1/PRSS1
Accession # P07477
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE)
under non-reducing conditions only
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Trypsin 1/PRSS1 (Catalog # 3848-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin 1/PRSS1

Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen (1). Constituting approximately two‑thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro‑enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).

  1. Emi, M. et al. (1986) Gene 41:305.
  2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
  3. Teich, N. et al. (2006) Hum. Mutat. 27:721.
Entrez Gene IDs
5644 (Human); 114228 (Mouse); 24691 (Rat)
Alternate Names
Beta-trypsin; Cationic trypsinogen; digestive zymogen; DKFZp779A0837; EC 3.4.21; EC; FLJ57296; MGC120175; MGC149362; nonfunctional trypsin 1; protease, serine, 1 (trypsin 1); PRSS1; PTRYI; Serine protease 1; TRP1; TRY1beta-trypsin; TRY4; Trygn16; TRYP1; Trypsin 1; Trypsin I; trypsin-1; Trypsinogen 1; trypsinogen A

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Citation for Human Trypsin 1/PRSS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Autoantibodies against the exocrine pancreas in autoimmune pancreatitis: gene and protein expression profiling and immunoassays identify pancreatic enzymes as a major target of the inflammatory process.
    Authors: Lohr JM, Faissner R, Koczan D, Bewerunge P, Bassi C, Brors B, Eils R, Frulloni L, Funk A, Halangk W, Jesenofsky R, Jesnowski R, Kaderali L, Kleeff J, Kruger B, Lerch MM, Losel R, Magnani M, Neumaier M, Nittka S, Sahin-Toth M, Sanger J, Serafini S, Schnolzer M, Thierse HJ, Wandschneider S, Zamboni G, Kloppel G
    Am. J. Gastroenterol., 2012;105(9):2060-71.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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