Human Trypsin 3/PRSS3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Trypsin 3/PRSS3 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Trypsin-1 or rhTrypsin-2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 420025
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Trypsin 3/PRSS3
    Val16-Ser247
    Accession # NP_002762
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize Recombinant Human Active Trypsin 3/PRSS3 (0.03 µg/mL, Catalog # 3714-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 0.15 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Neutralization of Trypsin 3/
PRSS3 Activity by Human Trypsin 3/PRSS3 Antibody.

The cleavage of Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002) by Recombinant Human Active Trypsin 3/PRSS3 (0.03 µg/mL, Catalog # 3714-SE) is measured after preincubation with increasing concentrations of Rat Anti-Human Trypsin 3/PRSS3 Monoclonal Antibody (Catalog # MAB3710). The ND50 is typically 0.15 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trypsin 3/PRSS3
Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).
  • References:
    1. Nyaruhucha, C. N. M. et al. (1997) J. Biol. Chem. 272:10573.
    2. Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
    3. Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
    4. Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.
  • Entrez Gene IDs:
    5646 (Human); 22073 (Mouse); 362347 (Rat)
  • Alternate Names:
    Brain trypsinogen; EC 3.4.21; EC 3.4.21.4; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; Trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV
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