Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1065916
View all TSG101 Primary Antibodies »

Product Specifications

Immunogen

TSG101 containing synthetic peptide
Accession # Q99816

Specificity

Detects TSG101 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TSG101 Antibody

Detection of Human TSG101 by Western Blot.

Western blot shows lysates of A172 human glioblastoma cell line, MDA‑MB‑231 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human TSG101 Monoclonal Antibody (Catalog # MAB114381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TSG101 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human TSG101 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: A172 human glioblastoma cell line, MDA‑MB‑231 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSG101

TSG101 is an essential member of the ESCRT-1 complex which regulates the sorting of ubiquitinated proteins to endosomes, facilitating vesicular trafficking and is implicated in normal development.  It is also involved in regulating transcription, protein sorting, biogenesis of multi-vesicular bodies, and viral budding.  Dysregulation of ESCRT proteins occurs in the development of various human diseases, including many types of cancers and neurodegenerative diseases.  TSG101 is an established cancer-associated gene and truncated aberrantly spliced mRNAs have been reported in various types of cancer. TSG101 is also commonly used as a marker protein for exosomes.

References

  1. Tufan AB, Lazarow K, Kolesnichenko M, Sporbert A, von Kries JP, Scheidereit C. TSG101 associates with PARP1 and is essential for PARylation and DNA damage-induced NF-kappa B activation. EMBO J. 2022 Nov 2;41(21):e110372.
    doi: 10.15252/embj.2021110372. Epub 2022 Sep 20. PMID: 36124865; PMCID: PMC9627669. 
  2. Chua HH, Kameyama T, Mayeda A, Yeh TH. Cancer-Specifically Re-Spliced TSG101 mRNA Promotes Invasion and Metastasis of Nasopharyngeal Carcinoma. Int J Mol Sci. 2019 Feb 12;20(3):773.
    doi: 10.3390/ijms20030773. PMID: 30759747; PMCID: PMC6387056. 
  3. Willms E, Johansson HJ, Mäger I, Lee Y, Blomberg KE, Sadik M, Alaarg A, Smith CI, Lehtiö J, El Andaloussi S, Wood MJ, Vader P. Cells release subpopulations of exosomes with distinct molecular and biological properties. Sci Rep. 2016 Mar 2;6:22519.
    doi: 10.1038/srep22519. PMID: 26931825; PMCID: PMC4773763.

Long Name

Tumor Susceptibility 101

Alternate Names

TSG10, VPS23

Entrez Gene IDs

7251 (Human)

Gene Symbol

TSG101

UniProt

Q99816 (Human)

Additional TSG101 Products

Product Documents for Human TSG101 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human TSG101 Antibody

For research use only

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