Human TSPAN33 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects HEK293 human embryonic kidney cell line transfected with human TSPAN33 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Mouse IgG2B Clone # 545422
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human TSPAN33
    Accession # NP_848657
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TSPAN33 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated overnight with Recombinant Human IL‑4 (Catalog # 204-IL) and Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL) were stained with Mouse Anti-Human TSPAN33 APC‑conjugated Monoclonal Antibody (Catalog # FAB8405A) and Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TSPAN33
TSPAN33 is a member of the tetraspanin family and is predominantly expressed in erythroblasts. It plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitors. TSPAN33 is expressed in several lymphomas including Hodgkin’s and Diffuse large B cell lymphoma. TSPAN33 is also expressed in some autoimmune diseases where B cells participate in the pathology.
  • Long Name:
    Tetraspanin 33
  • Entrez Gene IDs:
    340348 (Human); 232670 (Mouse); 500065 (Rat)
  • Alternate Names:
    hPen; PEN; Penumbra; proerythroblast new membrane; tetraspanin 33; tetraspanin-33; tspan-33
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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