Human Ubiquilin 1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Ubiquilin 1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Ubiquilin 1
    Arg546-Gly585
    Accession # Q9UMX0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Ubiquilin 1 by Western Blot. Western blot shows lysates of TT human medullary thyroid cancer cell line, IMR‑32 human neuroblastoma cell line, and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Ubiquilin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7969) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ubiquilin 1 at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ubiquilin 1

Ubiquilin-1 (also known as DA41 and PLIC-1/Protein Linking IAP with Cytoskeleton 1) is a 62-70 kDa intracellular member of the UBL-UBA family of proteins. Consistent with its name, it ubiquitously expressed, and is particularly prominent in skeletal muscle and neurons. Multiple functions have been attributed to ubiquilin-1. For instance, it is now known to be a key player in APP processing. On the cleavage side, ubiquilin-1 binds to PS/presenilin, a component of the gamma -secretase complex that cleaves APP. Ubiquilin-1 appears to target both ubiquitinated single chain PS to the proteosome, and aggregated PS to the aggresome. With respect to APP, ubiquilin-1 serves as a molecular chaperone, binding to and inhibiting APP aggregation. On cells expressing CD47, Ubiquilin-1 acts as an intermediary between integrins, the cytoskeleton and CD47, promoting cell spreading. And in neurons, ubiquilin-1 stabilizes both membrane and GABA subunit stability, enhancing inhibitory synaptic transmission. Human ubiquilin-1 is 589 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 37-111), an S5a binding site (aa 72-105) and a UBA binding region (aa 548-585). There are multiple splice variants, ranging from 19-59 kDa in size. One shows a deletion of aa 418-445, a second contains a three aa substitution for aa 151-589, a third possesses a 29 aa substitution for aa 112-589, and a fourth shows a deletion of aa 61-237 coupled to a second deletion of aa 361‑589. Over aa 546-585, human and mouse ubiquilin-1 are identical in amino acid sequence.

  • Entrez Gene IDs:
    29979 (Human); 56085 (Mouse); 114590 (Rat)
  • Alternate Names:
    DA41; DA41FLJ90054; DSK2; hPLIC-1; PLIC1; PLIC-1; PLIC-1UBQN; Protein linking IAP with cytoskeleton 1; Ubiquilin 1; ubiquilin-1; UBQLN1; UBQN; XDRP1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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