Human uPAR Quantikine ELISA Kit

(14 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
  • Sensitivity
    33 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human uPAR
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
Product Summary
The Quantikine Human uPAR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human uPAR in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPAR and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural human uPAR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human uPAR.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Standard Deviation17.365.418144.378.9136

To assess the linearity of the assay, samples spiked with high concentrations of uPAR were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human uPAR Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: uPAR
The urokinase-type plasminogen activator (uPA) is one of two activators that converts plasminogen to plasmin. uPA binds with high-affinity to the cell surface via the GPI-linked receptor uPAR, which serves to localize uPA proteolytic activity. Human uPAR cDNA encodes a 335 amino acid (aa) precursor protein with a 22 aa signal peptide, five potential N-linked glycosylation sites and a C-terminal GPI-anchor site.
    • Long Name
      Urokinase-type Plasminogen Activator Receptor
    • Entrez Gene IDs
      5329 (Human); 18793 (Mouse);
    • Alternate Names
      CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 14
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    Sample Type
    1. Soluble urokinase receptor is a biomarker of cardiovascular disease in chronic kidney disease.
      Authors: Meijers B, Poesen R, Claes K, Dietrich R, Bammens B, Sprangers B, Naesens M, Storr M, Kuypers D, Evenepoel P
      Kidney Int, 2015;87(1):210-6.
      Species: Human
      Sample Type: Serum
    2. Soluble Urokinase Receptor Is Released Selectively by Glioblastoma Cells That Express Epidermal Growth Factor Receptor Variant III and Promotes Tumor Cell Migration and Invasion.
      Authors: Gilder A, Jones K, Hu J, Wang L, Chen C, Carter B, Gonias S
      J Biol Chem, 2015;290(24):14798-809.
      Species: Human
      Sample Type: Serum
    3. Soluble Urokinase Receptor Levels Are Correlated with Focal Segmental Glomerulosclerosis Lesions in IgA Nephropathy: A Cohort Study from China.
      Authors: Guo S, Han M, Chen M, Ning Y, Pei G, Li Y, Dai W, Ge S, Deng Y, Guo Y, Li X, Haller H, Xu G, Rong S
      PLoS ONE, 2015;10(9):e0138718.
      Species: Human
      Sample Type: Plasma
    4. p-Cresol affects reactive oxygen species generation, cell cycle arrest, cytotoxicity and inflammation/atherosclerosis-related modulators production in endothelial cells and mononuclear cells.
      Authors: Chang M, Chang H, Chan C, Yeung S, Hsien H, Lin B, Yeh C, Tseng W, Tseng S, Jeng J
      PLoS ONE, 2014;9(12):e114446.
      Species: Human
      Sample Type: whole cells
    5. Serum-soluble urokinase receptor concentration in primary FSGS.
      Authors: Maas RJ, Wetzels JF, Deegens JK
      Kidney Int., 2012;81(10):1043-4.
      Species: Human
      Sample Type: Serum
    6. Circulating urokinase receptor as a cause of focal segmental glomerulosclerosis.
      Authors: Wei C, El Hindi S, Li J, Fornoni A, Goes N, Sageshima J, Maiguel D, Karumanchi SA, Yap HK, Saleem M, Zhang Q, Nikolic B, Chaudhuri A, Daftarian P, Salido E, Torres A, Salifu M, Sarwal MM, Schaefer F, Morath C, Schwenger V, Zeier M, Gupta V, Roth D, Rastaldi MP, Burke G, Ruiz P, Reiser J
      Nat. Med., 2011;17(8):952-60.
      Species: Human
      Sample Type: Serum
    7. Identification of regulatory Hck and PAI-2 proteins in the monocyte response to PEG-containing matrices.
      Authors: Zuckerman ST, Brown JF, Kao WJ
      Biomaterials, 2009;30(23):3825-33.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. The human airway trypsin-like protease modulates the urokinase receptor (uPAR, CD87) structure and functions.
      Authors: Beaufort N, Leduc D, Eguchi H, Mengele K, Hellmann D, Masegi T, Kamimura T, Yasuoka S, Fend F, Chignard M, Pidard D
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;292(5):L1263-72.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
      Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
      Am. J. Respir. Cell Mol. Biol., 2006;35(4):503-11.
      Species: Human
      Sample Type: BALF
    10. Low oxygen concentrations inhibit trophoblast cell invasion from early gestation placental explants via alterations in levels of the urokinase plasminogen activator system.
      Authors: Lash GE, Otun HA, Innes BA, Bulmer JN, Searle RF, Robson SC
      Biol. Reprod., 2005;74(2):403-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. The fibroblast growth factor receptor-4 Arg388 allele is associated with prostate cancer initiation and progression.
      Authors: Wang J, Stockton DW, Ittmann M
      Clin. Cancer Res., 2004;10(18):6169-78.
      Species: Human
      Sample Type: Cell Lysates
    12. Soluble urokinase-type plasminogen activator receptor (suPAR) as an independent factor predicting worse prognosis and extra-bone marrow involvement in multiple myeloma patients.
      Authors: Rigolin GM, Tieghi A, Ciccone M, Bragotti LZ, Cavazzini F, Della Porta M, Castagnari B, Carroccia R, Guerra G, Cuneo A, Castoldi G
      Br. J. Haematol., 2003;120(6):953-9.
      Species: Human
      Sample Type: Serum
    13. Pretreatment determination of the serum urokinase plasminogen activator and its soluble receptor in advanced small-cell lung cancer or non-small-cell lung cancer.
      Authors: Cobos E, Jumper C, Lox C
      Clin. Appl. Thromb. Hemost., 2003;9(3):241-6.
      Species: Human
      Sample Type: Serum
    14. The circulating urokinase plasminogen activator (uPA) and its soluble receptor (suPAR) are not up-regulated by the circulating P105 fraction of the HER-2/neu proto-oncogene: in vivo evidence from patients with advanced non-small cell lung cancer (NSCLC).
      Authors: Jumper C, Cobos E, Lox C
      Anticancer Res., 2002;22(4):2073-6.
      Species: Human
      Sample Type: Serum
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