Human Urinary TIM-1/KIM-1/HAVCR Quantikine ELISA Kit

  (10 citations)     
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Assay Procedure
Citations (10)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Urine (50 uL)
  • Sensitivity
    0.046 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Urine)
  • Specificity
    Natural and recombinant human TIM-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC24 , Quantikine Immunoassay Control Group 9 - Please Inquire
Product Summary
The Quantikine Human TIM-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIM-1 in urine. It contains NS0-expressed recombinant human TIM-1 and antibodies raised against the recombinant factor. Natural human TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIM-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.98 3.04 5.88 1.09 3.19 6.23
Standard Deviation 0.042 0.119 0.259 0.069 0.193 0.484
CV% 4.3 3.9 4.4 6.3 6 7.8

Recovery

The recovery of TIM-1 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=20) 104 94-112
Linearity
To assess the linearity of the assay, samples containing high concentrations of TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. For more information on linearity and hand
 TIM-1/KIM-1/HAVCR [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.
  • Long Name:
    T Cell Immunoglobulin Mucin-1
  • Entrez Gene IDs:
    26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
  • Alternate Names:
    CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Sample Type
  1. Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgery
    Authors: JJ Wang, NH Chi, TM Huang, R Connolly, LW Chen, SJ Chueh, WC Kan, CC Lai, VC Wu, JT Fang, TS Chu, KD Wu
    Crit Care, 2018;22(1):108.
    Species: Human
    Sample Type: Urine
  2. Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method
    Authors: Y Jin, X Shao, B Sun, C Miao, Z Li, Y Shi
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Type: Urine
  3. Angiopoietin/Tie2 Dysbalance Is Associated with Acute Kidney Injury after Cardiac Surgery Assisted by Cardiopulmonary Bypass.
    Authors: Jongman R, van Klarenbosch J, Molema G, Zijlstra J, de Vries A, van Meurs M
    PLoS ONE, 2015;10(8):e0136205.
    Species: Human
    Sample Type: Urine
  4. Autophagy activation reduces renal tubular injury induced by urinary proteins.
    Authors: Liu, Wei Jing, Luo, Mian-Na, Tan, Jin, Chen, Wei, Huang, Lei-zhao, Yang, Chen, Pan, Qingjun, Li, Benyi, Liu, Hua-feng
    Autophagy, 2014;10(2):243-56.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. A comparison of the ability of levels of urinary biomarker proteins and exosomal mRNA to predict outcomes after renal transplantation.
    Authors: Peake, Philip W, Pianta, Timothy, Succar, Lena, Fernando, Mangalee, Pugh, Debbie J, McNamara, Kathleen, Endre, Zoltan H
    PLoS ONE, 2014;9(2):e98644.
    Species: Human
    Sample Type: Urine
  6. Urinary kidney injury molecule-1 is related to pathologic involvement in IgA nephropathy with normotension, normal renal function and mild proteinuria.
    Authors: Xu P, Wei L, Shang W, Tian S, Gu D, Yan T, Lin S
    BMC Nephrol, 2014;15(0):107.
    Species: Human
    Sample Type: Urine
  7. Urinary N-acetyl-beta-D glucosaminidase as a surrogate marker for renal function in autosomal dominant polycystic kidney disease: 1 year prospective cohort study.
    BMC Nephrol, 2012;13(0):93.
    Species: Human
    Sample Type: Urine
  8. Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis.
    Authors: de Borst MH, Nauta FL, Vogt L, Laverman GD, Gansevoort RT, Navis G
    PLoS ONE, 2012;7(5):e37957.
    Species: Human
    Sample Type: Urine
  9. Measuring urinary tubular biomarkers in type 2 diabetes does not add prognostic value beyond established risk factors.
    Kidney Int, 2012;82(7):812-8.
    Species: Human
    Sample Type: Urine
  10. Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury.
    Authors: Metzger J, Kirsch T, Schiffer E, Ulger P, Mentes E, Brand K, Weissinger EM, Haubitz M, Mischak H, Herget-Rosenthal S
    Kidney Int., 2010;78(12):1252-62.
    Species: Human
    Sample Type: Urine

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