Human VASA Antibody

Catalog # Availability Size / Price Qty
AF2030
AF2030-SP
Human VASA Antibody in Western Blot
2 Images
Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (2)

Human VASA Antibody Summary

Species Reactivity
Human
Specificity
Detects human VASA in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human VASA
Met1-Tyr145
Accession # Q9NQI0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human VASA by Western Blot. View Larger

Detection of Human VASA by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry VASA in Human Testis. View Larger

VASA in Human Testis. VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VASA

The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).

References
  1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
  2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
  3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.
Long Name
DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41
Entrez Gene IDs
54514 (Human); 13206 (Mouse); 310090 (Rat)
Alternate Names
DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; EC 3.6.1; EC 3.6.4.13; MGC111074; probable ATP-dependent RNA helicase DDX4; Vasa homolog; VASA; VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Product Datasheets

Citations for Human VASA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice
    Authors: S Bagheri-Fa, H Chen, S Wilson, K Ayers, J Hughes, F Sloan-Bena, P Calvel, G Robevska, B Puisac, K Kusz-Zamel, S Gimelli, A Spik, J Jaruzelska, A Warenik-Sz, S Faradz, S Nef, J Pié, P Thomas, A Sinclair, D Wilhelm
    PLoS ONE, 2020;15(1):e0227411.
    Species: Mouse
    Sample Types: Tissue
    Applications: IF
  2. Quantitative proteomic profiling of the human ovary from early to mid-gestation reveals protein expression dynamics of oogenesis and folliculogenesis
    Authors: A Bothun, Y Gao, Y Takai, O Ishihara, H Seki, B Karger, J Tilly, DC Woods
    Stem Cells Dev., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells
    Authors: Á Vértesy, W Arindrarto, MS Roost, B Reinius, V Torrens-Ju, M Bialecka, I Moustakas, Y Ariyurek, E Kuijk, H Mei, R Sandberg, A van Oudena, SM Chuva de S
    Nat Commun, 2018;9(1):1873.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
    Authors: BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey
    Cell Rep, 2018;25(6):1650-1667.e8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance
    Authors: JM Stringer, SC Forster, Z Qu, L Prokopuk, MK O'Bryan, DK Gardner, SJ White, D Adelson, PS Western
    BMC Biol., 2018;16(1):104.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
    Authors: JJ Gell, J Zhao, D Chen, TJ Hunt, AT Clark
    Stem Cell Res, 2018;27(0):46-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility
    Authors: N Wang, C Satirapod, Y Ohguchi, ES Park, DC Woods, JL Tilly
    Sci Rep, 2017;7(1):10011.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23
    Authors: AT Clark, S Gkountela, D Chen, W Liu, E Sosa, M Sukhwani, JD Hennebold, KE Orwig
    Stem Cell Reports, 2017;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: ICC
  9. Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming
    Authors: C Eguizabal
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.
    Authors: Heeren A, van Iperen L, Klootwijk D, de Melo Bernardo A, Roost M, Gomes Fernandes M, Louwe L, Hilders C, Helmerhorst F, van der Westerlaken L, Chuva de Sousa Lopes S
    BMC Dev Biol, 2015;15(0):4.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling.
    Authors: Hu, Yueh-Chi, Nicholls, Peter K, Soh, Y Q Shir, Daniele, Joseph R, Junker, Jan Phil, van Oudenaarden, Alexande, Page, David C
    PLoS Genet, 2015;11(3):e1005019.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
    Authors: Lim J, Shim M, Lee J, Lee D
    PLoS ONE, 2014;9(4):e90454.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies.
    Authors: Dominguez, Antonia, Chiang, H Rosari, Sukhwani, Meena, Orwig, Kyle E, Reijo Pera, Renee A
    Sci Rep, 2014;4(0):6432.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  14. Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
    Authors: Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P
    Stem Cells Dev, 2013;22(23):3141-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?
    Authors: Bhartiya, Deepa, Sriraman, Kalpana, Gunjal, Pranesh, Modak, Harshada
    J Ovarian Res, 2012;5(1):32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Complete meiosis from human induced pluripotent stem cells.
    Authors: Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC
    Stem Cells, 2011;29(8):1186-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells.
    Authors: Geens M, Sermon KD
    J. Assist. Reprod. Genet., 2011;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
    Authors: West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL
    Stem Cells Dev., 2011;20(6):1079-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
    Authors: West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL
    Stem Cells, 2008;26(11):2768-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Isolation of primordial germ cells from differentiating human embryonic stem cells.
    Authors: Tilgner K, Atkinson SP, Golebiewska A, Stojkovic M, Lako M, Armstrong L
    Stem Cells, 2008;26(12):3075-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
    Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
    Stem Cells, 2008;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008;456(7220):344-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human VASA Antibody

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Human VASA Antibody
By Anonymous on 11/11/2019
Application: ICC/IF Sample Tested: Testis tissue Species: Mouse

DDX4/VASA germ cell marker. IF1:500


Human VASA Affinity Purified Polyclonal Ab
By Anonymous on 04/13/2016
Application: ICC/IF Sample Tested: Testis tissue Species: Mouse

Postnatal day 6 mouse testes were fixed in 4% paraformaldehyde. Tissue was embedded in O.C.T. and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (AF2030) was diluted in blocking buffer to 1.25 µg/ml and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate (Thermo Fisher A-21432) diluted to 1:500 for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100 and mounted.