Detection of Human VASA by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VASA in Human Testis. VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.
DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41
Entrez Gene IDs:
54514 (Human); 13206 (Mouse); 310090 (Rat)
DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; EC 3.6.1; EC 22.214.171.124; MGC111074; probable ATP-dependent RNA helicase DDX4; Vasa homolog; VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Generation of pluripotent stem cells from adult human testis. Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T Nature, 2008;456(7220):344-9. Species: Human Sample Type: Whole Cells Application: ICC
Immunocytochemistry/Immunofluorescence: Human VASA Affinity Purified Polyclonal Ab [AF2030]
Other Experimental Details
Other Experimental Details
Postnatal day 6 mouse testes were fixed in 4% paraformaldehyde. Tissue was embedded in O.C.T. and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (AF2030) was diluted in blocking buffer to 1.25 µg/ml and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate (Thermo Fisher A-21432) diluted to 1:500 for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100 and mounted.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.