Human VASA Antibody
Human VASA Antibody Summary
Met1-Tyr145
Accession # Q9NQI0
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Human VASA by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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VASA in Human Testis. VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VASA
The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
- Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
- Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
- Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.
Product Datasheets
Citations for Human VASA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Loss of NEDD4 causes complete XY gonadal sex reversal in mice
Authors: SP Windley, C Mayère, AE McGovern, NL Harvey, S Nef, Q Schwarz, S Kumar, D Wilhelm
Cell Death & Disease, 2022;13(1):75.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment
Authors: J Guo, E Sosa, T Chitiashvi, X Nie, EJ Rojas, E Oliver, DonorConne, K Plath, JM Hotaling, JB Stukenborg, AT Clark, BR Cairns
Cell Stem Cell, 2021;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice
Authors: S Bagheri-Fa, H Chen, S Wilson, K Ayers, J Hughes, F Sloan-Bena, P Calvel, G Robevska, B Puisac, K Kusz-Zamel, S Gimelli, A Spik, J Jaruzelska, A Warenik-Sz, S Faradz, S Nef, J Pié, P Thomas, A Sinclair, D Wilhelm
PLoS ONE, 2020;15(1):e0227411.
Species: Mouse
Sample Types: Tissue
Applications: IF -
PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation
Authors: JJ Gell, J Zhao, D Chen, TJ Hunt, AT Clark
Stem Cell Res, 2018;27(0):46-56.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells
Authors: Á Vértesy, W Arindrarto, MS Roost, B Reinius, V Torrens-Ju, M Bialecka, I Moustakas, Y Ariyurek, E Kuijk, H Mei, R Sandberg, A van Oudena, SM Chuva de S
Nat Commun, 2018;9(1):1873.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Quantitative proteomic profiling of the human ovary from early to mid-gestation reveals protein expression dynamics of oogenesis and folliculogenesis
Authors: A Bothun, Y Gao, Y Takai, O Ishihara, H Seki, B Karger, J Tilly, DC Woods
Stem Cells Dev., 2018;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
Authors: BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey
Cell Rep, 2018;25(6):1650-1667.e8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance
Authors: JM Stringer, SC Forster, Z Qu, L Prokopuk, MK O'Bryan, DK Gardner, SJ White, D Adelson, PS Western
BMC Biol., 2018;16(1):104.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23
Authors: AT Clark, S Gkountela, D Chen, W Liu, E Sosa, M Sukhwani, JD Hennebold, KE Orwig
Stem Cell Reports, 2017;0(0):.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: ICC -
Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility
Authors: N Wang, C Satirapod, Y Ohguchi, ES Park, DC Woods, JL Tilly
Sci Rep, 2017;7(1):10011.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming
Authors: C Eguizabal
Stem Cells, 2016;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling.
Authors: Hu, Yueh-Chi, Nicholls, Peter K, Soh, Y Q Shir, Daniele, Joseph R, Junker, Jan Phil, van Oudenaarden, Alexande, Page, David C
PLoS Genet, 2015;11(3):e1005019.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.
Authors: Heeren A, van Iperen L, Klootwijk D, de Melo Bernardo A, Roost M, Gomes Fernandes M, Louwe L, Hilders C, Helmerhorst F, van der Westerlaken L, Chuva de Sousa Lopes S
BMC Dev Biol, 2015;15(0):4.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies.
Authors: Dominguez, Antonia, Chiang, H Rosari, Sukhwani, Meena, Orwig, Kyle E, Reijo Pera, Renee A
Sci Rep, 2014;4(0):6432.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
Authors: Lim J, Shim M, Lee J, Lee D
PLoS ONE, 2014;9(4):e90454.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
Authors: Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P
Stem Cells Dev, 2013;22(23):3141-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?
Authors: Bhartiya, Deepa, Sriraman, Kalpana, Gunjal, Pranesh, Modak, Harshada
J Ovarian Res, 2012;5(1):32.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
Authors: West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL
Stem Cells Dev., 2011;20(6):1079-88.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells.
Authors: Geens M, Sermon KD
J. Assist. Reprod. Genet., 2011;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Complete meiosis from human induced pluripotent stem cells.
Authors: Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC
Stem Cells, 2011;29(8):1186-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Generation of pluripotent stem cells from adult human testis.
Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
Nature, 2008;456(7220):344-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
Authors: West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL
Stem Cells, 2008;26(11):2768-76.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Isolation of primordial germ cells from differentiating human embryonic stem cells.
Authors: Tilgner K, Atkinson SP, Golebiewska A, Stojkovic M, Lako M, Armstrong L
Stem Cells, 2008;26(12):3075-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
Stem Cells, 2008;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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DDX4/VASA germ cell marker. IF1:500
Postnatal day 6 mouse testes were fixed in 4% paraformaldehyde. Tissue was embedded in O.C.T. and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (AF2030) was diluted in blocking buffer to 1.25 µg/ml and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate (Thermo Fisher A-21432) diluted to 1:500 for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100 and mounted.
