Human VASA Antibody

(15 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human VASA in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human VASA
    Met1-Tyr145
    Accession # Q9NQI0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human VASA by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VASA at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
VASA in Human Testis. VASA was detected in paraffin-embedded sections of human testis using Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2030) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VASA
The VASA gene, originally identified in Drosophila, encodes a member of the DEAD box family of ATP-dependent RNA helicases. The expression of VASA in invertebrate and vertebrate species is restricted to germ cells and is required for germ cell formation. VASA is therefore a highly specific marker of germ cells (1-3).
  • References:
    1. Zeeman, A.M. et al. (2002) Lab Invest. 82:159.
    2. Raz, E. (2000) Genome Biol. 1:REVIEWS1017.
    3. Castrillon, D. et al. (2001) Proc. Natl. Acad. Sci. USA 97:9585.
  • Long Name:
    DEAD [Asp-Glu-Ala-Asp] Box Polypeptide 41
  • Entrez Gene IDs:
    54514 (Human); 13206 (Mouse); 310090 (Rat)
  • Alternate Names:
    DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; EC 3.6.1; EC 3.6.4.13; MGC111074; probable ATP-dependent RNA helicase DDX4; Vasa homolog; VASA; VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23
    Authors: AT Clark, S Gkountela, D Chen, W Liu, E Sosa, M Sukhwani, JD Hennebold, KE Orwig
    Stem Cell Reports, 2017;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: ICC
  2. Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming
    Authors: C Eguizabal
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  3. Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling.
    Authors: Hu, Yueh-Chi, Nicholls, Peter K, Soh, Y Q Shir, Daniele, Joseph R, Junker, Jan Phil, van Oudenaarden, Alexande, Page, David C
    PLoS Genet, 2015;11(3):e1005019.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.
    Authors: Heeren A, van Iperen L, Klootwijk D, de Melo Bernardo A, Roost M, Gomes Fernandes M, Louwe L, Hilders C, Helmerhorst F, van der Westerlaken L, Chuva de Sousa Lopes S
    BMC Dev Biol, 2015;15(0):4.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
    Authors: Lim J, Shim M, Lee J, Lee D
    PLoS ONE, 2014;9(4):e90454.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies.
    Authors: Dominguez, Antonia, Chiang, H Rosari, Sukhwani, Meena, Orwig, Kyle E, Reijo Pera, Renee A
    Sci Rep, 2014;4(0):6432.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential.
    Authors: Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P
    Stem Cells Dev, 2013;22(23):3141-55.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries?
    Authors: Bhartiya, Deepa, Sriraman, Kalpana, Gunjal, Pranesh, Modak, Harshada
    J Ovarian Res, 2012;5(1):32.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  9. Complete meiosis from human induced pluripotent stem cells.
    Authors: Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC
    Stem Cells, 2011;29(8):1186-95.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells.
    Authors: West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL
    Stem Cells Dev., 2011;20(6):1079-88.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells.
    Authors: Geens M, Sermon KD
    J. Assist. Reprod. Genet., 2011;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  12. Isolation of primordial germ cells from differentiating human embryonic stem cells.
    Authors: Tilgner K, Atkinson SP, Golebiewska A, Stojkovic M, Lako M, Armstrong L
    Stem Cells, 2008;26(12):3075-85.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
    Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
    Stem Cells, 2008;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Generation of pluripotent stem cells from adult human testis.
    Authors: Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T
    Nature, 2008;456(7220):344-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling.
    Authors: West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL
    Stem Cells, 2008;26(11):2768-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 15 Citations
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human VASA Affinity Purified Polyclonal Ab AF2030
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Excellent
 ICC/IF Mouse Anonymous 04/13/2016
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Image Details
Immunocytochemistry/Immunofluorescence Human VASA Affinity Purified Polyclonal Ab AF2030
Immunocytochemistry/Immunofluorescence: Human VASA Affinity Purified Polyclonal Ab [AF2030]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedTestis tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsPostnatal day 6 mouse testes were fixed in 4% paraformaldehyde. Tissue was embedded in O.C.T. and 5 micron sections cut. Sections were permeabilized with PBS containing 0.1% Triton X-100, then blocked for 30 min in 3% BSA in PBS containing 0.1% Triton X-100. Goat Anti-Human VASA Antigen Affinity-purified Polyclonal Antibody (AF2030) was diluted in blocking buffer to 1.25 µg/ml and applied to sections for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100, then incubated with secondary antibody: Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate (Thermo Fisher A-21432) diluted to 1:500 for 1 hour at RT. Sections were washed 3X with PBS containing 0.1% Triton X-100 and mounted.

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