Human Vasorin/SLIT-like 2 Antibody

Catalog # Availability Size / Price Qty
MAB2140
MAB2140-SP
Detection  of Human Vasorin/SLIT‑like 2 by Western Blot.
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Citations (1)
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Human Vasorin/SLIT-like 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Vasorin in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 227526
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Vasorin
aa 24-575
Accession # Q6EMK4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human Vasorin/SLIT‑like 2 by Western Blot. View Larger

Detection of Human Vasorin/SLIT‑like 2 by Western Blot. Western blot shows lysates of human placenta tissue and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vasorin/SLIT‑like 2 Monoclonal Antibody (Catalog # MAB2140) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Vasorin/SLIT‑like 2 at approximately 65 and 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vasorin/SLIT-like 2

Vasorin is a type I membrane protein that is predominantly expressed in vascular smooth muscle cells in a developmentally regulated pattern. Expression is down-regulated during vessel repair after arterial injury. Vasorin directly binds to transforming growth factor (TGF)-beta and attenuates TGF-beta signaling in vitro (1).

References
  1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
Entrez Gene IDs
114990 (Human); 246154 (Mouse)
Alternate Names
Atia; Protein slit-like 2; SLIT like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; Vasorin

Product Datasheets

Citation for Human Vasorin/SLIT-like 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
    Authors: Gunawardana CG, Kuk C, Smith CR
    J. Proteome Res., 2009;8(10):4705-13.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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