Detection of Human Vasorin/SLIT‑like 2 by Western Blot.
Western blot shows lysates of human placenta tissue and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vasorin/SLIT‑like 2 Monoclonal Antibody (Catalog # MAB2140) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Vasorin/SLIT‑like 2 at approximately 65 and 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vasorin/SLIT-like 2
Vasorin is a type I membrane protein that is predominantly expressed in vascular smooth muscle cells in a developmentally regulated pattern. Expression is down-regulated during vessel repair after arterial injury. Vasorin directly binds to transforming growth factor (TGF)-beta and attenuates TGF-beta signaling in vitro (1).
Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
Entrez Gene IDs:
114990 (Human); 246154 (Mouse)
Atia; Protein slit-like 2; SLIT like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; Vasorin
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