Human Vasorin/SLIT-like 2 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Vasorin in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 227526
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Vasorin
    aa 24-575
    Accession # Q6EMK4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Vasorin/SLIT‑like 2 by Western Blot. Western blot shows lysates of human placenta tissue and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vasorin/SLIT‑like 2 Monoclonal Antibody (Catalog # MAB2140) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Vasorin/SLIT‑like 2 at approximately 65 and 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vasorin/SLIT-like 2
Vasorin is a type I membrane protein that is predominantly expressed in vascular smooth muscle cells in a developmentally regulated pattern. Expression is down-regulated during vessel repair after arterial injury. Vasorin directly binds to transforming growth factor (TGF)-beta and attenuates TGF-beta signaling in vitro (1).
  • References:
    1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
  • Entrez Gene IDs:
    114990 (Human); 246154 (Mouse)
  • Alternate Names:
    Atia; Protein slit-like 2; SLIT like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; vasorin; Vasorin/SLIT-like 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
    Authors: Gunawardana CG, Kuk C, Smith CR
    J. Proteome Res., 2009;8(10):4705-13.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 99  
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Mouse IgG1 Isotype Control

Ctrl MAB002R
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Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF007 16  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 8  
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Donkey Anti-Mouse IgG NL557 Affinity Purified PAb

Flow, IHC, ICC NL007 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 6
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Mouse IgG Antibody

WB AF007 3
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 1
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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