Human VCAM-1/CD106 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: VCAM-1/CD106

VCAM-1, also known as CD106, is an immunoglobulin (Ig)-like adhesion molecule that is mainly expressed in endothelial cells and other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes (1, 2). It plays a critical role in inflammation by recruiting leukocytes to acute and chronic inflammation sites (3, 4). Alternatively-spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 aa extracellular domain (ECD) that includes seven C2-type immunoglobulin domains, a 22 aa transmembrane segment, and a 19 amino acid (aa) cytoplasmic tail. Within the ECD, human VCAM-1 shares 75% and 76% aa sequence identity with the mouse and rat VCAM-1, respectively. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation (5). ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals, but elevated levels of serum VCAM-1 are indicative of future Atrial Fibrillation incident as well as liver disease (6, 7). Tumor cells use overexpression of VCAM-1 as means of escaping immune surveillance (8).

Product Datasheets

Related Research Areas

• Acute Myeloid Leukemia
• Adhesion Molecules in Angiogenesis
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Adhesive Interactions in Allergy and Asthma
• Adipose-derived Stem Cell Markers
• Cancer Biomarkers
• Endothelial Cell Differentiation Markers
• Endothelial Cell Markers
• Endothelial Progenitor and Endothelial Cell Markers
• Hematopoietic Stem Cell Markers
• Immunoglobulin Superfamily CAMs
• Mesenchymal Stem Cell Markers
• Mouse Tissue-Resident Macrophage Markers