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Human VCAM-1/CD106 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VCAM-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Human VCAM-1 / CD106 ELISA Standard Curve

Product Datasheets

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VCAM-1/CD106

VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.

Long Name:
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs:
7412 (Human); 22329 (Mouse)
Alternate Names:
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Assay Procedure


Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human VCAM-1/CD106 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. The effect of transdermal gender-affirming hormone therapy on markers of inflammation and hemostasis
    Authors: MH Schutte, R Kleemann, NM Nota, CM Wiepjes, JM Snabel, G T'Sjoen, A Thijs, M den Heijer
    PLoS ONE, 2022;17(3):e0261312.
    Species: Human
    Sample Types: Plasma
  2. The effect of moderate wine consumption on cytokine secretion by peripheral blood mononuclear cells: A randomized clinical study in coronary heart disease patients
    Authors: E Fragopoulo, C Argyrou, M Detopoulou, S Tsitsou, S Seremeti, M Yannakouli, S Antonopoul, G Kolovou, P Kalogeropo
    Cytokine, 2021;146(0):155629.
    Species: Human
    Sample Types: Plasma
  3. Predictors of Early-Recurrence Atrial Fibrillation after Catheter Ablation in Women and Men with Abnormal Body Weight
    Authors: J Budzianows, J Hiczkiewic, K ?ojewska, E Kawka, R Rutkowski, K Korybalska
    Journal of Clinical Medicine, 2021;10(12):.
    Species: Human
    Sample Types: Serum
  4. Complement activation is associated with poor outcome after out-of-hospital cardiac arrest
    Authors: V Chaban, ER Nakstad, H Stær-Jense, C Schjalm, I Seljeflot, J Vaage, C Lundqvist, JŠ Benth, K Sunde, TE Mollnes, GØ Andersen, SE Pischke
    Resuscitation, 2021;0(0):.
    Species: Human
    Sample Types: Plasma
  5. Urinary proteomics links keratan sulfate degradation and lysosomal enzymes to early type 1 diabetes
    Authors: JAD Van, S Clotet-Fre, AC Hauschild, I Batruch, I Jurisica, Y Elia, FH Mahmud, E Sochett, EP Diamandis, JW Scholey, A Konvalinka
    PLoS ONE, 2020;15(5):e0233639.
    Species: Human
    Sample Types: Urine
  6. Urinary soluble VCAM-1 is a useful biomarker of disease activity and treatment response in lupus nephritis
    Authors: AA Gasparin, NPB de Andrade, V Hax, PE Palominos, M Siebert, R Marx, PG Schaefer, FV Veronese, OA Monticielo
    BMC rheumatology, 2020;4(1):67.
    Species: Human
    Sample Types: Urine
  7. Soluble VCAM-1 promotes gemcitabine resistance via macrophage infiltration and predicts therapeutic response in pancreatic cancer
    Authors: R Takahashi, H Ijichi, M Sano, K Miyabayash, D Mohri, J Kim, G Kimura, T Nakatsuka, H Fujiwara, K Yamamoto, Y Kudo, Y Tanaka, K Tateishi, Y Nakai, Y Morishita, K Soma, N Takeda, HL Moses, H Isayama, K Koike
    Scientific Reports, 2020;10(1):21194.
    Species: Human
    Sample Types: Plasma
  8. The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis
    Authors: P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford
    Sci Rep, 2019;9(1):8348.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Blood inflammatory and endothelial markers in women with von Willebrand disease
    Authors: I Govorov, K Bremme, A Larsson, M Holmström, E Komlichenk, R Chaireti, M Mints
    PLoS ONE, 2019;14(1):e0210544.
    Species: Human
    Sample Types: Plasma
  10. Urinary angiostatin, CXCL4 and VCAM-1 as biomarkers of lupus nephritis
    Authors: CC Mok, S Soliman, LY Ho, FA Mohamed, FI Mohamed, C Mohan
    Arthritis Res. Ther., 2018;20(1):6.
    Species: Human
    Sample Types: Urine
  11. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018;2018(0):8286067.
    Species: Human
    Sample Types: Serum
  12. Biomarkers of endothelial dysfunction predict sepsis mortality in young infants: a matched�case-control study
    Authors: JK Wright, K Hayford, V Tran, GM Al Kibria, A Baqui, A Manajjir, A Mahmud, N Begum, M Siddiquee, KC Kain, A Farzin
    BMC Pediatr, 2018;18(1):118.
    Species: Human
    Sample Types: Plasma
  13. Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4
    Authors: M Du, A Martin, F Hays, J Johnson, RA Farjo, KM Farjo
    Mol. Vis., 2017;23(0):185-197.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Serum Biomarkers of Myocardial Remodeling and Coronary Dysfunction in Early Stages of Hypertrophic Cardiomyopathy in the Young
    Authors: E Fernlund, T Gyllenhamm, R Jablonowsk, M Carlsson, A Larsson, J Ärnlöv, P Liuba
    Pediatr Cardiol, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  15. Excess atherosclerosis in systemic lupus erythematosus,-A matter of renal involvement: Case control study of 281 SLE patients and 281 individually matched population controls
    Authors: JT Gustafsson, M Herlitz Li, I Gunnarsson, S Pettersson, K Elvin, J Öhrvik, A Larsson, K Jensen-Urs, E Svenungsso
    PLoS ONE, 2017;12(4):e0174572.
    Species: Human
    Sample Types: Tissue Homogenates
  16. Serum from Varicose Patients Induces Senescence-Related Dysfunction of Vascular Endothelium Generating Local and Systemic Proinflammatory Conditions
    Oxid Med Cell Longev, 2016;2016(0):2069290.
    Species: Human
    Sample Types: Serum
  17. Urine VCAM-1 as a marker of renal pathology activity index in lupus nephritis.
    Authors: Singh S, Wu T
    Oncogene, 2012;14(4):R164.
    Species: Human
    Sample Types: Urine
  18. Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.
    Authors: Pitteri SJ, JeBailey L, Faca VM, Thorpe JD, Silva MA, Ireton RC, Horton MB, Wang H, Pruitt LC, Zhang Q, Cheng KH, Urban N, Hanash SM, Dinulescu DM
    PLoS ONE, 2009;4(11):e7916.
    Species: Human
    Sample Types: Plasma
  19. Suspension microarrays for the identification of the response patterns in hyperinflammatory diseases.
    Authors: Hsu HY, Wittemann S, Schneider EM, Weiss M, Joos TO
    Med Eng Phys, 2008;30(8):976-83.
    Species: Human
    Sample Types: Plasma
  20. Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
    Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
    J. Immunol., 2007;179(10):7166-75.
    Species: Human
    Sample Types: Serum
  21. Cardiovascular effects of intravenous ghrelin infusion in healthy young men.
    Authors: Vestergaard ET, Andersen NH, Hansen TK, Rasmussen LM, Moller N, Sorensen KE, Sloth E, Jorgensen JO
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(5):H3020-6.
    Species: Human
    Sample Types: Serum
  22. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
    Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
    Br. J. Cancer, 2006;94(4):524-31.
    Species: Human
    Sample Types: Serum


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Reviews for Human VCAM-1/CD106 DuoSet ELISA

Average Rating: 4.4 (Based on 5 Reviews)

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Human VCAM-1/CD106 DuoSet ELISA
By Anonymous on 10/24/2019
Sample Tested: Serum and Plasma

Used in Serum and EDTA Plasma at 1:10 dilution, the sample may need further dilution, some times we get high %CV.

Human VCAM-1/CD106 DuoSet ELISA
By Anonymous on 03/07/2019
Sample Tested: Urine

Human VCAM-1/CD106 DuoSet ELISA
By Sophie Millar on 01/16/2019
Sample Tested: Cell Culture Media

Human VCAM-1/CD106 DuoSet ELISA
By BOCHATON Thomas on 06/14/2017
Sample Tested: Serum

We used human serum at a dilution of 1:2000. Excellent result.

Human VCAM-1/CD106 DuoSet ELISA
By Jonatan Dereke on 12/07/2016
Sample Tested: EDTA Plasma

We run human plasma samples at a 1:1000 dilution in this assay.