Human VEGF-C DuoSet ELISA

Catalog # Availability Size / Price Qty
DY752B
Ancillary Products Available
Human VEGF-C ELISA Standard Curve
1 Image
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews

Human VEGF-C DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF-C. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human VEGF-C ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF-C

Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Long Name:
Vascular Endothelial Growth Factor C
Entrez Gene IDs:
7424 (Human); 22341 (Mouse)
Alternate Names:
Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM

Citations for Human VEGF-C DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Superantigenic Activation of Human Cardiac Mast Cells
    Authors: G Varricchi, S Loffredo, F Borriello, A Pecoraro, F Rivellese, A Genovese, G Spadaro, G Marone
    Int J Mol Sci, 2019;20(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. VEGFC acts as a double-edged sword in renal cell carcinoma aggressiveness
    Authors: PD Ndiaye, M Dufies, S Giuliano, L Douguet, R Grépin, J Durivault, P Lenormand, N Glisse, J Mintcheva, V Vouret-Cra, B Mograbi, M Wurmser, D Ambrosetti, N Rioux-Lecl, P Maire, G Pagès
    Theranostics, 2019;9(3):661-675.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development
    Authors: J Bourland, J Fradette, FA Auger
    Sci Rep, 2018;8(1):13191.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Effects of proton versus photon irradiation on (lymph)angiogenic, inflammatory, proliferative and anti-tumor immune responses in head and neck squamous cell carcinoma
    Authors: M Lupu-Plesu, A Claren, S Martial, PD N'Diaye, K Lebrigand, N Pons, D Ambrosetti, I Peyrottes, J Feuillade, J Hérault, M Dufies, J Doyen, G Pagès
    Oncogenesis, 2017;6(7):e354.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Tuberculosis-diabetes co-morbidity is characterized by heightened systemic levels of circulating angiogenic factors.
    Authors: Kumar N, Moideen K, Sivakumar S, Menon P, Viswanathan V, Kornfeld H, Babu S
    J Infect, 2017;74(1):10-21.
    Species: Human
    Sample Types: Plasma
  6. Circulating Angiogenic Factors as Biomarkers of Disease Severity and Bacterial Burden in Pulmonary Tuberculosis.
    Authors: Kumar N, Banurekha V, Nair D, Babu S
    PLoS ONE, 2016;11(1):e0146318.
    Species: Human
    Sample Types: Plasma
  7. The CXCL7/CXCR1/2 axis is a key driver in the growth of clear cell renal cell carcinoma.
    Authors: Grepin R, Guyot M, Giuliano S, Boncompagni M, Ambrosetti D, Chamorey E, Scoazec J, Negrier S, Simonnet H, Pages G
    Cancer Res, 2014;74(3):873-83.
    Species: Human
    Sample Types: Tissue Culture Supernates

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