Human VEGF DuoSet ELISA

Catalog # Availability Size / Price Qty
DY293B
DY293B-05
Ancillary Products Available
Human VEGF ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (83)
FAQs
Supplemental Products
Reviews (7)

Human VEGF DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

  

Data Example

Human VEGF ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis.

Long Name:
Vascular Endothelial Growth Factor
Entrez Gene IDs:
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names:
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human VEGF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

83 Citations: Showing 1 - 10
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  1. Novel oncogenic and chemoresistance-inducing functions of resistin in ovarian cancer cells require miRNAs-mediated induction of epithelial-to-mesenchymal transition
    Authors: L Qiu, GF Zhang, L Yu, HY Wang, XJ Jia, TJ Wang
    Sci Rep, 2018;8(1):12522.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Serum from patients with chronic obstructive pulmonary disease induces senescence-related phenotype in bronchial epithelial cells
    Authors: B Ku?nar-Kam, J Miku?a-Pie, A Witucka, A Romaniuk, N Konieczna, B Rubi?, K Ksi??ek, A Tykarski, H Batura-Gab
    Sci Rep, 2018;8(1):12940.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Transplantation of Allogeneic Pericytes Improves Myocardial Vascularization and Reduces Interstitial Fibrosis in a Swine Model of Reperfused Acute Myocardial Infarction
    Authors: VV Alvino, R Fernández-, I Rodriguez-, S Slater, G Mangialard, E Avolio, H Spencer, L Culliford, S Hassan, L Sueiro Bal, A Herman, A Ayaon-Alba, C Galán-Arri, J Sánchez-Go, H Hennessey, C Delmege, R Ascione, C Emanueli, GD Angelini, B Ibanez, P Madeddu
    J Am Heart Assoc, 2018;7(2):.
    Species: Procine
    Sample Types: Cell Culture Supernates
  4. Comparative Analysis of Different Platelet Lysates and Platelet Rich Preparations to Stimulate Tendon Cell Biology: An In Vitro Study
    Authors: F Klatte-Sch, T Schmidt, M Uckert, S Scheffler, U Kalus, M Rojewski, H Schrezenme, A Pruss, B Wildemann
    Int J Mol Sci, 2018;19(1):.
    Species: Human
    Sample Types: Plasma
  5. A combination of platelet features allows detection of early-stage cancer
    Authors: S Sabrkhany, MJE Kuijpers, SMJ van Kuijk, L Sanders, S Pineda, SWM Olde Damin, AC Dingemans, AW Griffioen, MGA Oude Egbri
    Eur. J. Cancer, 2017;80(0):5-13.
    Species: Human
    Sample Types: Platelets
  6. ADAM9 promotes lung cancer progression through vascular remodeling by VEGFA, ANGPT2, and PLAT
    Authors: CY Lin, CF Cho, ST Bai, JP Liu, TT Kuo, LJ Wang, YS Lin, CC Lin, LC Lai, TP Lu, CY Hsieh, CN Chu, DC Cheng, YP Sher
    Sci Rep, 2017;7(1):15108.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Mechanisms regulating angiogenesis underlie seasonal control of pituitary function
    Authors: J Castle-Mil, DO Bates, DJ Tortonese
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2514-E2523.
    Species: Sheep
    Sample Types: Tissue Homogenates
  8. Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia
    Authors: Q Meng, Y Qin, M Deshpande, F Kashiwabuc, M Rodrigues, Q Lu, H Ren, JH Elisseeff, GL Semenza, SV Montaner, A Sodhi
    Invest. Ophthalmol. Vis. Sci., 2017;58(11):4514-4523.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Resveratrol reverses the adverse effects of bevacizumab on cultured ARPE-19 cells
    Authors: M Subramani, M Ponnalagu, L Krishna, N Jeyabalan, P Chevour, A Sharma, C Jayadev, R Shetty, N Begum, G Archunan, D Das
    Sci Rep, 2017;7(1):12242.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro
    Authors: E Redondo-Ca, C Cunningham, J Miller, L Martuscell, S Aoulad-Ali, NJ Rothwell, CM Kielty, SM Allan, E Pinteaux
    Stem Cell Res Ther, 2017;8(1):79.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. The Proangiogenic Capabilities of Malignant Ascites Generated by Aggressive Ovarian Tumors
    Authors: J Miku?a-Pie, P Uruski, S Szubert, K Maksin, R Moszy?ski, D Szpurek, A Wo?niak, S Sajdak, A Tykarski, K Ksi??ek
    Biomed Res Int, 2017;2017(0):2592496.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Reduction of relative centrifugal forces increases growth factor release within solid platelet-rich-fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept)
    Authors: K El Bagdadi, A Kubesch, X Yu, S Al-Maawi, A Orlowska, A Dias, P Booms, E Dohle, R Sader, CJ Kirkpatric, J Choukroun, S Ghanaati
    Eur J Trauma Emerg Surg, 2017;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  13. Effects of Ranibizumab and Aflibercept on Human M�ller Cells and Photoreceptors under Stress Conditions
    Authors: W Shen, B Yau, SR Lee, L Zhu, M Yam, MC Gillies
    Int J Mol Sci, 2017;18(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Effect of 2-octylcyanoacrylate on placenta derived mesenchymal stromal cells on extracellular matrix
    Authors: YJ Chen, L Lankford, S Kabagambe, Z Saenz, P Kumar, D Farmer, A Wang
    Placenta, 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Collagen-Based Medical Device as a Stem Cell Carrier for Regenerative Medicine
    Authors: L Aubert, M Dubus, H Rammal, C Bour, C Mongaret, C Boulagnon-, R Garnotel, C Schneider, R Rahouadj, C Laurent, SC Gangloff, F Velard, C Mauprivez, H Kerdjoudj
    Int J Mol Sci, 2017;18(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. The Effect of Aquaporin 1-Inhibition on Vasculogenic Mimicry in Malignant Mesothelioma
    Authors: E Pulford, J McEvoy, A Hocking, S Prabhakara, K Griggs, S Klebe
    Int J Mol Sci, 2017;18(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Tuberculosis-diabetes co-morbidity is characterized by heightened systemic levels of circulating angiogenic factors.
    Authors: Kumar N, Moideen K, Sivakumar S, Menon P, Viswanathan V, Kornfeld H, Babu S
    J Infect, 2017;74(1):10-21.
    Species: Human
    Sample Types: Plasma
  18. Gelatin device for the delivery of growth factors involved in endochondral ossification
    Authors: LA Ahrens, D Vonwil, J Christense, VP Shastri
    PLoS ONE, 2017;12(4):e0175095.
    Species: Chicken
    Sample Types: Cell Culture Supernates
  19. Is vascular endothelial growth factor a useful biomarker in giant cell arteritis?
    Authors: N Goodfellow, J Morlet, S Singh, A Sabokbar, A Hutchings, V Sharma, J Vaskova, S Masters, A Zarei, R Luqmani
    RMD Open, 2017;3(1):e000353.
    Species: Human
    Sample Types: Serum
  20. Tumor microenvironment conditions alter Akt and Na(+)/H(+) exchanger NHE1 expression in endothelial cells more than hypoxia alone: implications for endothelial cell function in cancer
    Authors: AK Pedersen, J Mendes Lop, N Mørup, K Tritsaris, SF Pedersen
    BMC Cancer, 2017;17(1):542.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Deletion of lactate dehydrogenase-A in myeloid cells triggers antitumor immunity
    Authors: P Seth, E Csizmadia, A Hedblom, M Vuerich, H Xie, M Li, MS Longhi, B Wegiel
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Minocycline modulates NF?B phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells
    Authors: AD Guerra, WE Rose, P Hematti, WJ Kao
    Stem Cell Res Ther, 2017;8(1):171.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Delphinidin inhibits angiogenesis through the suppression of HIF-1? and VEGF expression in A549 lung cancer cells
    Oncol. Rep, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Angiogenic proteins, placental weight and perinatal outcomes among pregnant women in Tanzania
    PLoS ONE, 2016;11(12):e0167716.
    Species: Human
    Sample Types: Plasma
  25. Regulating VEGF signaling in platelet concentrates via specific VEGF sequestering
    Authors: DG Belair, NN Le, WL Murphy
    Biomater Sci, 2016;4(5):819-25.
    Species: Human
    Sample Types: Plasma
  26. Stand-Sit Microchip for High-Throughput, Multiplexed Analysis of Single Cancer Cells
    Sci Rep, 2016;6(0):32505.
    Applications: High Throughput Chip Assay
  27. A functional bioassay to determine the activity of anti-VEGF antibody therapy in blood of patients with cancer
    Br J Cancer, 2016;0(0):.
    Species: Human
    Sample Types: Serum
  28. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
    Authors: Gabriela Odent Grig
    J Tissue Eng Regen Med, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Differential regulation of blood flow induced neovascularisation and mural cell recruitment by VEGF and angiopoietin signalling
    Authors: David O Bates
    J. Physiol. (Lond.), 2016;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. Circulating Angiogenic Factors as Biomarkers of Disease Severity and Bacterial Burden in Pulmonary Tuberculosis.
    Authors: Kumar N, Banurekha V, Nair D, Babu S
    PLoS ONE, 2016;11(1):e0146318.
    Species: Human
    Sample Types: Plasma
  31. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016;7(0):12329.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Effect of prostaglandin D2 on VEGF release by nasal polyp fibroblasts
    Authors: K Kanai, M Okano, T Fujiwara, S Kariya, T Haruna, R Omichi, SI Makihara, Y Hirata, K Nishizaki
    Allergol Int, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Synchronous down-modulation of miR-17 family members is an early causative event in the retinal angiogenic switch.
    Authors: Nunes D, Dias-Neto E, Cardo-Vila M, Edwards J, Dobroff A, Giordano R, Mandelin J, Brentani H, Hasselgren C, Yao V, Marchio S, Pereira C, Passetti F, Calin G, Sidman R, Arap W, Pasqualini R
    Proc Natl Acad Sci U S A, 2015;112(12):3770-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. A method to investigate the anti-metabolic activity of anti-cancer agents on ovarian cancer cells cultured in a 96-well high throughput format.
    Authors: Hogg S, Evans J, Sykes P, Chitcholtan K
    J Ovarian Res, 2015;8(0):43.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. The effect of nitrogen containing bisphosphonates, zoledronate and alendronate, on the production of pro-angiogenic factors by osteoblastic cells.
    Authors: Ishtiaq S, Edwards S, Sankaralingam A, Evans B, Elford C, Frost M, Fogelman I, Hampson G
    Cytokine, 2015;71(2):154-60.
    Species: Human
    Sample Types: Serum
  36. Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
    Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
    PLoS ONE, 2015;10(9):e0137612.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. High-grade serous ovarian cancer cell lines exhibit heterogeneous responses to growth factor stimulation.
    Authors: Bourgeois D, Kabarowski K, Porubsky V, Kreeger P
    Cancer Cell Int, 2015;15(0):112.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  39. Circulating autoantibodies to endothelial progenitor cells: binding characteristics and association with risk factors for atherosclerosis.
    Authors: George J, Matucci-Cerinic M, Bar I, Shimoni S
    PLoS ONE, 2014;9(6):e97836.
    Species: Human
    Sample Types: Serum
  40. Celastrol inhibits the HIF-1alpha pathway by inhibition of mTOR/p70S6K/eIF4E and ERK1/2 phosphorylation in human hepatoma cells.
    Authors: Ma J, Han L, Liang H, Mi C, Shi H, Lee J, Jin X
    Oncol Rep, 2014;32(1):235-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Prognostic impact of PHIP copy number in melanoma: linkage to ulceration.
    Authors: Bezrookove, Vladimir, De Semir, David, Nosrati, Mehdi, Tong, Schuyler, Wu, Clayton, Thummala, Suresh, Dar, Altaf A, Leong, Stanley, Cleaver, James E, Sagebiel, Richard, Miller, James R, Kashani-Sabet, Mohammed
    J Invest Dermatol, 2014;134(3):783-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014;14(0):35.
    Species: Human
    Sample Types: Serum
  43. hERG1 channels regulate VEGF-A secretion in human gastric cancer: clinicopathological correlations and therapeutical implications.
    Authors: Crociani O, Lastraioli E, Boni L, Pillozzi S, Romoli M, D'Amico M, Stefanini M, Crescioli S, Masi A, Taddei A, Bencini L, Bernini M, Farsi M, Beghelli S, Scarpa A, Messerini L, Tomezzoli A, Vindigni C, Morgagni P, Saragoni L, Giommoni E, Gasperoni S, Di Costanzo F, Roviello F, De Manzoni G, Bechi P, Arcangeli A
    Clin Cancer Res, 2014;20(6):1502-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Autocrine impact of VEGF-A on uveal melanoma cells.
    Authors: Koch K, Refaian N, Hos D, Schlereth S, Bosch J, Cursiefen C, Heindl L
    Invest Ophthalmol Vis Sci, 2014;55(4):2697-704.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. The radiosensitising effect of gemcitabine and its main metabolite dFdU under low oxygen conditions is in vitro not dependent on functional HIF-1 protein.
    Authors: Wouters A, Pauwels B, Burrows N, Baay M, Deschoolmeester V, Vu T, Laukens K, Meijnders P, Van Gestel D, Williams K, Van den Weyngaert D, Vermorken J, Pauwels P, Peeters M, Lardon F
    BMC Cancer, 2014;14(0):594.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Transplantation of human adipose tissue-derived stem cells delays clinical onset and prolongs life span in ALS mouse model.
    Authors: Kim, Kwang S, Lee, Hong J, An, Jin, Kim, Yun B, Ra, Jung Cha, Lim, Inja, Kim, Seung U
    Cell Transplant, 2014;23(12):1585-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. The role of VEGF-A165b in trophoblast survival.
    Authors: Bills V, Hamdollah-Zadeh M, Soothill P, Harper S, Bates D
    BMC Pregnancy Childbirth, 2014;14(0):278.
    Species: Human
    Sample Types: Cell Lysates
  48. Benefits of whole body vibration training in patients hospitalised for COPD exacerbations - a randomized clinical trial.
    Authors: Greulich T, Nell C, Koepke J, Fechtel J, Franke M, Schmeck B, Haid D, Apelt S, Filipovic S, Kenn K, Janciauskiene S, Vogelmeier C, Koczulla A
    BMC Pulm Med, 2014;14(0):60.
    Species: Human
    Sample Types: Serum
  49. Fcgamma receptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies.
    Authors: Justiniano S, Elavazhagan S, Fatehchand K, Shah P, Mehta P, Roda J, Mo X, Cheney C, Hertlein E, Eubank T, Marsh C, Muthusamy N, Butchar J, Byrd J, Tridandapani S
    J Biol Chem, 2013;288(37):26800-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage.
    Authors: Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L
    J Biol Chem, 2013;288(5):3097-111.
    Species: Human
    Sample Types: CSF
  51. IL-27 inhibits epithelial-mesenchymal transition and angiogenic factor production in a STAT1-dominant pathway in human non-small cell lung cancer.
    Authors: Kachroo P, Lee M, Zhang L, Baratelli F, Lee G, Srivastava M, Wang G, Walser T, Krysan K, Sharma S, Dubinett S, Lee J
    J Exp Clin Cancer Res, 2013;32(0):97.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. Fibroblasts derived from human pluripotent stem cells activate angiogenic responses in vitro and in vivo.
    Authors: Shamis Y, Silva E, Hewitt K, Brudno Y, Levenberg S, Mooney D, Garlick J
    PLoS ONE, 2013;8(12):e83755.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Abnormal histone methylation is responsible for increased vascular endothelial growth factor 165a secretion from airway smooth muscle cells in asthma.
    Authors: Clifford RL, John AE, Brightling CE
    J. Immunol., 2012;189(2):819-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma.
    J. Exp. Med., 2012;209(11):1985-2000.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  56. Increased levels of plasma haemoxygenase-1 in prostate cancer.
    Authors: Blann AD, Balakrishnan B, Ryan P
    Prostate Cancer Prostatic Dis., 2011;14(2):114-7.
    Species: Human
    Sample Types: Plasma
  57. Distinct platelet packaging, release, and surface expression of proangiogenic and antiangiogenic factors on different platelet stimuli.
    Authors: Chatterjee M, Huang Z, Zhang W, Jiang L, Hultenby K, Zhu L, Hu H, Nilsson GP, Li N
    Blood, 2011;117(14):3907-11.
  58. The roles of Wnt signaling modulators Dickkopf-1 (Dkk1) and Dickkopf-2 (Dkk2) and cell maturation state in osteogenesis on microstructured titanium surfaces.
    Authors: Olivares-Navarrete R, Hyzy S, Wieland M, Boyan BD, Schwartz Z
    Biomaterials, 2010;31(8):2015-24.
    Species: Human
    Sample Types: Cell Lysates
  59. Normal ranges of angiogenesis regulatory proteins in human platelets.
    Authors: Peterson JE, Zurakowski D, Italiano JE
    Am. J. Hematol., 2010;85(7):487-93.
    Species: Human
    Sample Types: Cell Lysates
  60. Viral G protein-coupled receptor up-regulates Angiopoietin-like 4 promoting angiogenesis and vascular permeability in Kaposi's sarcoma.
    Authors: Ma T, Jham BC, Hu J
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(32):14363-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Endothelium-based biomarkers are associated with cerebral malaria in Malawian children: a retrospective case-control study.
    Authors: Conroy AL, Phiri H, Hawkes M, Glover S, Mallewa M, Seydel KB, Taylor TE, Molyneux ME, Kain KC
    PLoS ONE, 2010;5(12):e15291.
    Species: Human
    Sample Types: Plasma
  62. Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
    Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
    FASEB J., 2010;24(10):3782-95.
    Species: Human
    Sample Types: Cell Lysates
  63. Angiogenesis and lymphangiogenesis are downregulated in primary breast cancer.
    Authors: Boneberg EM, Legler DF, Hoefer MM, Ohlschlegel C, Steininger H, Fuzesi L, Beer GM, Dupont-Lampert V, Otto F, Senn HJ, Furstenberger G
    Br. J. Cancer, 2009;101(4):605-14.
    Species: Human
    Sample Types: Serum
  64. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009;125(2):367-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Serum levels of angiogenic factors and their prognostic relevance in bladder cancer.
    Authors: Szarvas T, Jager T, Droste F, Becker M, Kovalszky I, Romics I, Ergun S, Rubben H
    Pathol. Oncol. Res., 2009;15(2):193-201.
    Species: Human
    Sample Types: Serum
  66. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009;183(12):8176-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.
    Authors: Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ
    Br. J. Cancer, 2009;101(7):1183-93.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
    Authors: Kurago ZB, Lam-ubol A, Stetsenko A
    Head Neck Pathol, 2008;2(1):1-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  69. Adenosine receptors in regulation of dendritic cell differentiation and function.
    Authors: Novitskiy SV, Ryzhov S, Zaynagetdinov R, Goldstein AE, Huang Y, Tikhomirov OY, Blackburn MR, Biaggioni I, Carbone DP, Feoktistov I, Dikov MM
    Blood, 2008;112(5):1822-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
    Authors: Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(38):14692-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Bone marrow cells are a rich source of growth factors and cytokines: implications for cell therapy trials after myocardial infarction.
    Authors: Korf-Klingebiel M, Kempf T, Sauer T, Brinkmann E, Fischer P, Meyer GP, Ganser A, Drexler H, Wollert KC
    Eur. Heart J., 2008;29(23):2851-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
    Authors: Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC
    Clin. Immunol., 2007;124(2):158-64.
    Species: Human
    Sample Types: Serum
  74. The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
    Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
    Clin. Sci., 2006;110(5):575-85.
    Species: Human
    Sample Types: Tissue Homogenates
  75. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
    Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
    Br. J. Cancer, 2006;94(4):524-31.
    Species: Human
    Sample Types: Serum
  76. Vascular endothelial growth factor-D is a survival factor for human breast carcinoma cells.
    Authors: Akahane M, Akahane T, Matheny SL, Shah A, Okajima E, Thorgeirsson UP
    Int. J. Cancer, 2006;118(4):841-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  77. Angiopoietin-2 levels are elevated in exudative pleural effusions.
    Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
    Chest, 2006;129(5):1259-66.
    Species: Human
    Sample Types: Pleural Effusion
  78. Pretreatment serum endostatin as a prognostic indicator in metastatic gastric carcinoma.
    Authors: Woo IS, Kim KA, Jeon HM, Hong SH, Rho SY, Koh SJ, Lee MA, Byun JH, Kang JH, Hong YS, Lee KS, Cho CS, Choi MG, Chung IS
    Int. J. Cancer, 2006;119(12):2901-6.
    Species: Human
    Sample Types: Serum
  79. The effects of dexamethasone and dehydroepiandrosterone (DHEA) on cytokines and receptor expression in a human osteoblastic cell line: potential steroid-sparing role for DHEA.
    Authors: Harding G, Mak YT, Evans B, Cheung J, MacDonald D, Hampson G
    Cytokine, 2006;36(1):57-68.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Hypoxia-induced increase in soluble Flt-1 production correlates with enhanced oxidative stress in trophoblast cells from the human placenta.
    Authors: Li H, Gu B, Zhang Y, Lewis DF, Wang Y
    Placenta, 2005;26(2):210-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. GM-CSF induces expression of soluble VEGF receptor-1 from human monocytes and inhibits angiogenesis in mice.
    Authors: Eubank TD, Roberts R, Galloway M, Wang Y, Cohn DE, Marsh CB
    Immunity, 2004;21(6):831-42.
    Species: Human
    Sample Types: Cell Culture Supernates
  82. Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction.
    Authors: Massa M, Rosti V, Ferrario M, Campanelli R, Ramajoli I, Rosso R, De Ferrari GM, Ferlini M, Goffredo L, Bertoletti A, Klersy C, Pecci A, Moratti R, Tavazzi L
    Blood, 2004;105(1):199-206.
    Species: Human
    Sample Types: Plasma
  83. Targeting SRPK1 to control VEGF-mediated tumour angiogenesis in metastatic melanoma.
    Authors: Gammons, M V, Lucas, R, Dean, R, Coupland, S E, Oltean, S, Bates, D O
    Br J Cancer, 0;111(3):477-85.
    Species: Human
    Sample Types: Cell Lysates

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Human VEGF DuoSet ELISA
By Anonymous on 10/11/2018
Sample Tested: Cell culture supernatant

Human VEGF DuoSet ELISA
By Anonymous on 06/07/2018
Sample Tested: Cell culture medium samples,medium samples

Great kit, very easy to follow!


Human VEGF DuoSet ELISA
By Anonymous on 06/23/2017
Sample Tested: A549 human lung carcinoma cell line

Human VEGF DuoSet ELISA
By Anonymous on 05/03/2017
Sample Tested: Human tissue

The substrate incubation resulted in weak change of color and so longer incubation is required for human VEGF ELISA kit.


Human VEGF DuoSet ELISA
By Anonymous on 03/14/2017
Sample Tested: Various cell lines

VEGF quantification was performed onto various cell lines and data obtained is presented with the chart attached


Human VEGF DuoSet ELISA
By Anonymous on 08/13/2016
Sample Tested: Cell culture supernatant

Human VEGF DuoSet ELISA
By Anonymous on 05/23/2016
Sample Tested: Various cell lines,Various cell lines were cultured and supernatant obtained to study time course VEGF secretion