Human VEGFR1/Flt-1 DuoSet ELISA

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Human VEGF R1 / Flt-1 ELISA Standard Curve
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Product Details
Citations (26)
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Reviews (2)

Human VEGFR1/Flt-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
125.0 - 8,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human VEGF R1 / Flt-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human VEGF R1 / Flt-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

Long Name:
Vascular Endothelial Growth Factor Receptor 1
Entrez Gene IDs:
2321 (Human); 14254 (Mouse)
Alternate Names:
EC 2.7.10; EC; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

Citations for Human VEGFR1/Flt-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Hydroxychloroquine reduces soluble Flt-1 secretion from human cytotrophoblast, but does not mitigate markers of endothelial dysfunction in vitro
    Authors: E Kadife, N Hannan, A Harper, N Binder, S Beard, FC Brownfoot
    PLoS ONE, 2022;17(11):e0271560.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Serum Collected from Preeclamptic Pregnancies Drives Vasoconstriction of Human Omental Arteries-A Novel Ex Vivo Model of Preeclampsia for Therapeutic Development
    Authors: BR Fato, N de Alwis, S Beard, NK Binder, N Pritchard, S Tong, TJ Kaitu'u-Li, NJ Hannan
    International Journal of Molecular Sciences, 2022;23(18):.
    Species: Human
    Sample Types: Serum
  3. No association between intravenous fluid volume and endothelial glycocalyx shedding in patients undergoing resuscitation for sepsis in the emergency department
    Authors: S Macdonald, E Bosio, NI Shapiro, L Balmer, S Burrows, M Hibbs, T Jowitt, L Smart, G Arendts, D Fatovich
    Scientific Reports, 2022;12(1):8733.
    Species: Human
    Sample Types: Serum
  4. A prospective cohort study providing insights for markers of adverse pregnancy outcome in older mothers
    Authors: SC Lean, RL Jones, SA Roberts, AEP Heazell
    BMC pregnancy and childbirth, 2021;21(1):706.
    Species: Human
    Sample Types: Serum
  5. NR4A2 expression is not altered in placentas from cases of growth restriction or preeclampsia, but is reduced in hypoxic cytotrophoblast
    Authors: N de Alwis, S Beard, NK Binder, N Pritchard, TJ Kaitu'u-Li, SP Walker, O Stock, KM Groom, S Petersen, A Henry, JM Said, S Seeho, SC Kane, S Tong, NJ Hannan
    Scientific Reports, 2021;11(1):20670.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Megalin, Proton Pump Inhibitors and the Renin-Angiotensin System in Healthy and Pre-Eclamptic Placentas
    Authors: Y Sun, L Tan, RI Neuman, M Broekhuize, S Schoenmake, X Lu, AHJ Danser
    International Journal of Molecular Sciences, 2021;22(14):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Identifying potential biomarkers related to pre-term delivery by proteomic analysis of amniotic fluid
    Authors: S Hong, JE Lee, YM Kim, Y Park, JW Choi, KH Park
    Sci Rep, 2020;10(1):19648.
    Species: Human
    Sample Types: Amniotic Fluid
  8. TLR3 expression by maternal and fetal cells at the maternal-fetal interface in normal and preeclamptic pregnancies
    Authors: LM Gierman, GB Silva, Z Pervaiz, JJ Rakner, SB Mundal, AJ Thaning, I Nervik, M Elschot, S Mathew, LCV Thomsen, L Bjørge, AC Iversen
    J. Leukoc. Biol., 2020;0(0):.
    Species: Human
    Sample Types: Placental Supernates
  9. Differential regulation of sFlt-1 splicing by U2AF65 and JMJD6 in placental-derived and endothelial cells
    Authors: AC Eddy, H Chapman, DT Brown, EM George
    Biosci. Rep., 2020;40(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. No association between early antiretroviral therapy during pregnancy and plasma levels of angiogenic factors: a cohort study
    Authors: A Djeha, S Girard, H Trottier, F Kakkar, H Soudeyns, M Boucher, N Lapointe, I Boucoiran
    BMC Pregnancy Childbirth, 2019;19(1):482.
    Species: Human
    Sample Types: Serum
  11. Sulfasalazine reduces placental secretion of antiangiogenic factors, up-regulates the secretion of placental growth factor and rescues endothelial dysfunction
    Authors: FC Brownfoot, NJ Hannan, P Cannon, V Nguyen, R Hastie, LJ Parry, S Senadheera, L Tuohey, S Tong, TJ Kaitu'u-Li
    EBioMedicine, 2019;0(0):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  12. Melatonin enhances antioxidant molecules in the placenta, reduces secretion of soluble fms-like tyrosine kinase 1 (sFLT) from primary trophoblast but does not rescue endothelial dysfunction: An evaluation of its potential to treat preeclampsia
    Authors: NJ Hannan, NK Binder, S Beard, TV Nguyen, TJ Kaitu'u-Li, S Tong
    PLoS ONE, 2018;13(4):e0187082.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Combinations of Bevacizumab and Erlotinib show activity in colorectal cancer independent of RAS status
    Authors: P Mésange, A Bouygues, N Ferrand, M Sabbah, AE Escargueil, A Savina, B Chibaudel, C Tournigand, T Andre, A de Gramont, AK Larsen
    Clin. Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
  14. Tuberculosis-diabetes co-morbidity is characterized by heightened systemic levels of circulating angiogenic factors.
    Authors: Kumar N, Moideen K, Sivakumar S, Menon P, Viswanathan V, Kornfeld H, Babu S
    J Infect, 2017;74(1):10-21.
    Species: Human
    Sample Types: Plasma
  15. Placental Dysfunction Underlies Increased Risk of Fetal Growth Restriction and Stillbirth in Advanced Maternal Age Women
    Authors: SC Lean, AEP Heazell, MR Dilworth, TA Mills, RL Jones
    Sci Rep, 2017;7(1):9677.
    Species: Human
    Sample Types: Serum
  16. Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
    Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
    Oncol Lett, 2017;13(5):3269-3276.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Circulating Angiogenic Factors as Biomarkers of Disease Severity and Bacterial Burden in Pulmonary Tuberculosis.
    Authors: Kumar N, Banurekha V, Nair D, Babu S
    PLoS ONE, 2016;11(1):e0146318.
    Species: Human
    Sample Types: Plasma
  18. Angiogenic proteins, placental weight and perinatal outcomes among pregnant women in Tanzania
    PLoS ONE, 2016;11(12):e0167716.
    Species: Human
    Sample Types: Plasma
  19. Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
    Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
    PLoS ONE, 2015;10(8):e0134619.
    Species: Human
    Sample Types: Plasma
  20. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014;14(0):35.
    Species: Human
    Sample Types: Serum
  21. Serum concentrations of soluble Flt-1 are decreased among women with a viable fetus and no symptoms of miscarriage destined for pregnancy loss.
    Authors: Kaitu'u-Lino TJ, Whitehead CL, Ngian GL, Permezel M, Tong S
    PLoS ONE, 2012;7(2):e32509.
    Species: Human
    Sample Types: Serum
  22. Differential expression of Vegfr-2 and its soluble form in preeclampsia.
    Authors: Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noel A, Goffin F, Tsatsaris V, Subtil D, Foidart JM
    PLoS ONE, 2012;7(3):e33475.
    Species: Human
    Sample Types: Plasma
  23. Combinations of Host Biomarkers Predict Mortality among Ugandan Children with Severe Malaria: A Retrospective Case-Control Study.
    Authors: Erdman LK, Dhabangi A, Musoke C, Conroy AL, Hawkes M, Higgins S, Rajwans N, Wolofsky KT, Streiner DL, Liles WC, Cserti-Gazdewich CM, Kain KC
    PLoS ONE, 2011;6(2):e17440.
    Species: Human
    Sample Types: Plasma
  24. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  25. Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy.
    Authors: Lash GE, Naruse K, Innes BA, Robson SC, Searle RF, Bulmer JN
    Placenta, 2010;31(6):545-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009;183(12):8176-85.
    Species: Human
    Sample Types: Cell Culture Supernates


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Human VEGFR1/Flt-1 DuoSet ELISA
By Anonymous on 08/17/2020
Sample Tested: Pregnancy serum

Human VEGF R1/Flt-1 DuoSet ELISA
By Anonymous on 09/25/2017
Sample Tested: HEK293T human embryonic kidney cell line,Tissue Culture Media