Human ZSCAN21 Antibody

Catalog # Availability Size / Price Qty
AF8015
AF8015-SP
Human ZSCAN21 Antibody in Data
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Human ZSCAN21 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ZSCAN21 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ZSCAN21
Asn139-Asp223
Accession # Q9Y5A6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human ZSCAN21 antibody by Western Blot. View Larger

Detection of Human ZSCAN21 by Western Blot. Western blot shows lysates of Caki-2 human clear cell carcinoma epithelial cell line, COLO 205 human colorectal adenocarcinoma cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ZSCAN21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8015) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ZSCAN21 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZSCAN21

ZSCAN21 (Zinc finger and SCAN domain-containing protein 21; also Zfp-38, NY-REN-21, Zipro1 and Ru49) is a nuclear member of the multi-finger SCAN box protein family. It is expressed in select cell types, including cerebellar granule cells, Schwann cells, cerebral cortical and sympathetic neurons, round spermatids, and cells of the hair follicles plus sebaceous glands. It may have multiple functions, one of which is to initiate transcription of neuronal alpha -synuclein, while a second may be to promote granule cell proliferation. Human ZSCAN21 is 473 amino acids (aa) in length. It contains one SCAN (SREZBP, Ctfin51, AW-1/ ZNF174, and Number 18) domain (aa 45-127) that mediates dimerization, followed by seven consecutive C2H2-type zinc-finger regions (aa 277-466). ZSCAN21 is known to form both noncovalent homodimers and heterodimers with SCAND1. There is one potential isoform variant that shows a 188 aa substitution for aa 263-473. Over aa 139-223, human ZSCAN21 shares 54% aa sequence identity with mouse ZSCAN21.

Long Name
Zinc Finger and SCAN Domain Containing 21
Entrez Gene IDs
7589 (Human)
Alternate Names
DKFZp434L134; KOX25; NY-REN-21; Renal carcinoma antigen NY-REN-21; ZFP38; zfp-38; ZFP383; zinc finger and SCAN domain containing 21; zinc finger and SCAN domain-containing protein 21; zinc finger protein 38 (KOX 25); Zinc finger protein 38 homolog; zinc finger protein 38; zinc finger protein NY-REN-21 antigen; Zipro1; ZNF38; ZNF38DKFZp686H10254; ZSCAN21

Product Datasheets

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