HuR/ELAVL1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-03449
![Western Blot: HuR/ELAVL1 AntibodyBSA Free [NBP3-03449]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-Western-Blot-NBP3-03449-img0008.jpg "Western Blot: HuR/ELAVL1 AntibodyBSA Free [NBP3-03449]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human HuR/ELAVL1 (NP_001410.2). MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HuR/ELAVL1 Antibody - BSA Free
Western Blot: HuR/ELAVL1 AntibodyBSA Free [NBP3-03449]
Western Blot: HuR/ELAVL1 Antibody [NBP3-03449] - Analysis of extracts of various cell lines, using HuR/ELAVL1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.![Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03449-img0001.jpg "Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]")
Immunocytochemistry/ Immunofluorescence: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]
Immunocytochemistry/Immunofluorescence: HuR/ELAVL1 Antibody [NBP3-03449] - Analysis of MCF-7 cells using HuR/ELAVL1 antibody. Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-Immunohistochemistry-Paraffin-NBP3-03449-img0006.jpg "Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]")
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody [NBP3-03449] - Paraffin-embedded human esophageal cancer using HuR/ELAVL1 antibody at dilution of 1:200 (40x lens).![Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-Immunohistochemistry-Paraffin-NBP3-03449-img0002.jpg "Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]")
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody [NBP3-03449] - Paraffin-embedded mouse testis using HuR/ELAVL1 antibody at dilution of 1:200 (40x lens).![Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-Immunohistochemistry-Paraffin-NBP3-03449-img0004.jpg "Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]")
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]
Immunohistochemistry-Paraffin: HuR/ELAVL1 Antibody [NBP3-03449] - Paraffin-embedded rat testis using HuR/ELAVL1 antibody at dilution of 1:200 (40x lens).![Knockout Validated: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]](https://resources.rndsystems.com/images/products/HuR-ELAVL1-Antibody-Knockout-Validated-NBP3-03449-img0007.jpg "Western Blot: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]")
Western Blot: HuR/ELAVL1 Antibody - BSA Free [NBP3-03449]
Western Blot: HuR/ELAVL1 Antibody [NBP3-03449] - Analysis of extracts from normal (control) and HuR / ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Applications for HuR/ELAVL1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:100-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HuR/ELAVL1
Alternate Names
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), ELAV-like protein 1, embryonic lethal, abnormal vision, drosophila, homolog-like 1, Hu antigen R, Hua, Hu-antigen R, HuR, HURELAV1, MelG
Gene Symbol
ELAVL1
Additional HuR/ELAVL1 Products
Product Documents for HuR/ELAVL1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HuR/ELAVL1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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