ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-22541

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Proximity Ligation Assay, Electron Microscopy

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Flow Cytometry, Simple Western, Proximity Ligation Assay, IF/IHC, Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1A29

Format

Azide and BSA Free
View all ICAM-1/CD54 Primary Antibodies »

Product Specifications

Immunogen

Rat lymph node stroma

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

ICAM-1-CD54-Antibody-1A29-Western-Blot-NBP2-22541-img0015.jpg
Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry-Frozen: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of frozen rat spleen with Mouse anti Rat CD54.
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in C6 cells (green) compared to an isotype control (blue).
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse kidney tissue.
Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse lung tissue.
Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normal biopsies of deparaffinized Mouse spleen tissue.
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Staining of stimulated rat spleen cells with mouse anti-rat CD54 (Alexa488).
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Ramos cells (green) compared to an isotype control (blue).
Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541]

Flow Cytometry: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Analysis of CD54 in Raji cells (green) compared to an isotype control (blue).
ICAM-1/CD54 Antibody (1A29)

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] -

Knockdown of RPN2 decreased luminal B BC cell dissemination in vivo. Luminal B ER+ T47D cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting RPN2 (siRNA-RPN2) were injected + estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos with green fluorescent blood vessels & analyzed as described in materials & methods. (A) Migration in vitro (n = 6). (B)In vivo dissemination of transfected T47D in presence of E2 ± Neu (n = 23–26). Scale bar = 100 µm. (n = 23–26). (C) Western blot analysis for confirmation of siRNA-RPN2 transfection & VCAM-1, ICAM-1, & MUC-1 expression. (D) Focal adhesions area (n = 5–6). Scale bar = 10 µm. (E) Proliferation (n = 6). Representative images of zebrafish embryos with disseminated luminal B T47D BC cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated T47D & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, ***P < 0.001, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ICAM-1/CD54 Antibody (1A29)

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] -

Knockdown of U2AF1 decreased luminal A BC cell dissemination in vivo. Luminal A ER+ MCF-7 cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting U2AF1 (siRNA-U2AF1) were injected in presence of estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos, with green fluorescent blood vessels, & analyzed as described in materials & methods. (A) Migration in vitro (n = 6–12). (B)In vivo dissemination in presence of E2 ± Neu (n = 38–41). Scale bar = 100 µm. (C) Western blot analysis for confirmation of siRNA-U2AF1 transfection & ICAM-1, VCAM-1, & MUC-1 expression. (D) Focal adhesion area (n = 7). Scale bar = 10 µm. (E) Proliferation in vitro (n = 12). Representative images of zebrafish embryos with disseminated MCF-7 cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated MCF-7 cells & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, **P < 0.01, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ICAM-1/CD54 Antibody (1A29)

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] -

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Normalized gene & protein expression of ICAM-1. (A) qPCR results indicated no change in mRNA expression of ICAM-1 at 24 h post-overpressure but a significant increase at 48 h as compared to sham. (B) Subsequent Western blot results confirmed elevated ICAM-1 protein levels at 48 h compared to sham. *p < 0.05, Data are represented as mean ± SEM, gene: n = 7–9/group, protein: n = 8–9/group. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30853931), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ICAM-1/CD54 Antibody (1A29)

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] -

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated, compared to single exposure, radiation more profoundly enhanced ICAM-1 expression. Representative (3 independent experiments) Western blot analysis & quantification of ICAM-1 levels in whole-cell lysates from nonirradiated (sham) & irradiated HUVECs 4 h & 24 h after exposure to (a) either five fractions of 2 Gy (5 × 2 Gy) or single exposure to 10 Gy & (b) either five fractions of 2.5 Gy (5 × 2.5 Gy) or single exposure to 12.5 Gy. Fractions delivered at 24-h intervals. beta -actin served as a loading control. c Ectopic expression of ICAM-1 after exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy, as measured by flow cytometry (n = 3). d NF-kappa B activation after 4 h of exposure to 0 Gy, five fractions of 2 Gy, or 10 Gy (n = 2). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ICAM-1/CD54 Antibody (1A29)

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] -

Western Blot: ICAM-1/CD54 Antibody (1A29) [NBP2-22541] - Fractionated thoracic irradiation suppressed KLF2 & enhanced ICAM-1 levels in the lung. Quantification of KLF2 (a) & ICAM-1 (b) protein levels & representative Western blot analysis (c) in the lung tissue of mice (n = 6) at 24 h following 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. beta -actin served as a loading control. KLF2 immunostaining in the lung tissue samples of sham irradiated (d), irradiated (e), & quantitation (f) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. ICAM-1 immunostaining in the lung tissue samples of sham irradiated (g), or irradiated (h), & quantitation (i) at 24 h after exposure to 5 fractions of 4 Gy at 24 h intervals or single exposure to 20 Gy. (n, number of independent experiments performed; a, significant statistical difference between nonirradiated & irradiated groups; b, significant statistical difference between fractionated irradiation & single exposure; *p < 0.05; **p < 0.01; ***p < 0.001). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32382091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1 - 3 ug/ml
Application Notes
Use in PLA reported in scientific literature (PMID:34572594). Use in Immunohistochemistry free floating, and Electron Microscopy reported in scientific literature (PMID: 8574890)..
See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size

Reviewed Applications

Read 1 review rated 4 using NBP2-22541 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ICAM-1/CD54

ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

15894 (Mouse)

Gene Symbol

ICAM1

Additional ICAM-1/CD54 Products

Product Documents for ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

Customer Reviews for ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free (1)

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  • ICAM-1/CD54 Antibody (1A29)
    Name: James Xiao
    Application: Immunohistochemistry-Frozen
    Sample Tested: Lung tissue
    Species: Mouse lung and Mouse
    Verified Customer | Posted 03/20/2017
    Frozen mouse tissue staining with ICAM-1 ab (NBP-2-22541)
    Frozen section of the mouse lung was stained with 1:100 dilution of ICAM-1 ab (NBP-2-22541) for 1 hr RT. Tissue was washed and stained with Alexa-594 Goat anti-mouse antibody for an additional of 30 minutes RT. Section was then washed and analyzed by fluorescence microbe.
    ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free NBP2-22541

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Protocols

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FAQs for ICAM-1/CD54 Antibody (1A29) - Azide and BSA Free

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  • Q: Our customer found it difficult to choose between NBP2-22541 and NB100-65608. They will perform IHC-P with muscle sections from rat. Would you please help recommend one?

    A:

    Both NBP2-22541 and NB100-65608 are covered by our 100% guarantee for IHC-P with rat tissue. Please find more information about our quality guarantee, which you may wish to pass on to your customer. Your customer may be interested in our antibody with catalogue number NB500-318. This has also been validated for IHC-P with rat tissue, and furthermore has a publication citing its use for IHC-P with rat heart tissue. Here is a link to the product page of this antibody NB500-318.

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