IFIT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-46052

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IFIT2 Primary Antibodies »

Product Specifications

Immunogen

interferon-induced protein with tetratricopeptide repeats 2. (Uniprot# P09913)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IFIT2 Antibody - BSA Free

IFIT2 Antibody - BSA Free

Immunohistochemistry: IFIT2 Antibody - BSA Free [NBP3-46052] -

Immunohistochemistry: IFIT2 Antibody - BSA Free [NBP3-46052] - Immunohistochemistry of paraffin-embedded human kidney tissue slide using (IFIT2 Antibody) at dilution of 1:50
IFIT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody - BSA Free [NBP3-46052] -

Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody - BSA Free [NBP3-46052] - Immunofluorescent analysis of HepG2 cells using (IFIT2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
IFIT2 Antibody - BSA Free

Western Blot: IFIT2 Antibody - BSA Free [NBP3-46052] -

Western Blot: IFIT2 Antibody - BSA Free [NBP3-46052] - A431 cells were subjected to SDS PAGE followed by western blot with (IFIT2 Antibody) at dilution of 1:300

Applications for IFIT2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunohistochemistry

1:50-1:500

Western Blot

1:200-1:1000
Application Notes
Theoretical Molecular Weight is 50-55 kDa

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IFIT2

Saha et al. used this antibody to study protein expression of murine GARG39/IFIT2 in relation to viral infection. Immunofluorescence localized this protein in association with the mitotic spindle in both mitotically active normal NIH3T3 and B16F10 melanoma cells. Western blotting shows approx. 55 kD band GARG39/IFIT2 in brain tissue of mice infected with Japanese encephalitis virus (JEV). Brain tissue from non-infected mice did not display this band. In 1994 Bluyssen et al. characterized the gene for murine GARG39/IFIT2 and historically the protein has been identified as interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), interferon-induced 54 kDa protein (IFI-54K), glucocorticoid-attenuated response gene 39 protein (GARG-39). Human IFIT2, with 62% amino acid sequence identity, has been referred to as Interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), Interferon-induced 54 kDa protein (IFI-54K) and ISG-54 K. Kang et al. showed with proteome analysis IFIT2 was expressed more than two-fold in human osteosarcoma cells U2OS treated with ascochlorin. Kawada et al. studied gene expression to show that IFIT2 is strongly upregulated in peripheral blood in children during the acute phase of influenza.

Alternate Names

cig42, G10P2IFI-54K, GARG-39, IFI-54, IFI54IFIT-2, IFI-54K, Interferon, alpha-inducible protein (MW 54kD), Interferon-induced 54 kDa protein, interferon-induced protein 54, interferon-induced protein with tetratricopeptide repeats 2, ISG54, ISG-54 K, ISG-54K, P54

Gene Symbol

IFIT2

Additional IFIT2 Products

Product Documents for IFIT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for IFIT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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