IFN-alpha 1 Antibody

Novus Biologicals | Catalog # NBP2-75930

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all IFN-alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within mouse IFN-alpha 1 aa 140-181. (SwissProt: P01562 Human; SwissProt: P01572 Mouse; SwissProt: P05011 Rat)

Localization

Secreted.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IFN-alpha 1 Antibody

Western Blot: IFN-alpha 1 Antibody [NBP2-75930]

Western Blot: IFN-alpha 1 Antibody [NBP2-75930]

Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930]

Immunocytochemistry/ Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930]

Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930]

Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930]

Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
Flow Cytometry: IFN-alpha 1 Antibody [NBP2-75930]

Flow Cytometry: IFN-alpha 1 Antibody [NBP2-75930]

Flow Cytometry: IFN-alpha 1 Antibody [NBP2-75930] - analysis of SH-SY5Y cells with IFNA1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930]

Immunocytochemistry/ Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930]

Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930]

Immunocytochemistry/ Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930]

Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930]

Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930]

Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded rat brain tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930]

Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930]

Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded human kidney tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
IFN-alpha 1 Antibody

Immunocytochemistry/ Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] -

(A) show the expression and distribution of representative multicolored immunofluorescence of IFN alpha, IFNRA, IP10, IRF3, IRF5, and IRF7 in liver tissue of the deceased twin and control groups. IFN alpha, IFNAR, and IP10 as Panel 1(green represents IP10, yellow represents IFN alpha, orange represents IFNRA, pink represents CD68, and blue represents the nucleus), and IRF3, IRF5, and IRF7 as Panel 2(green represents IRF7, yellow represents IRF5, orange represents IRF3, pink represents CD68 and blue represents the nucleus). (B) displayed statistical analysis based on (A). (C) displayed IP10 and IFN alpha correlation with IRF7, IRF5, IRF3, and IFNAR, respectively. N.S, no significace, * P value <0.05, ** P value <0.01, *** P value <0.001 and **** P value <0.001. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fcimb.2024.1391824/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IFN-alpha 1 Antibody

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFN-alpha 1

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name

Interferon alpha 1

Alternate Names

IFNA1, IFNalpha 1

Gene Symbol

IFNA1

Additional IFN-alpha 1 Products

Product Documents for IFN-alpha 1 Antibody

Certificate of Analysis

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Product Specific Notices for IFN-alpha 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

Citations for IFN-alpha 1 Antibody

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Protocols

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