IFN-alpha 2 Antibody (N27) - BSA Free

Novus Biologicals | Catalog # NBP3-18803

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # N27

Format

BSA Free
View all IFN-alpha 2/IFNA2 Primary Antibodies »

Product Specifications

Immunogen

E.coli derived recombinant human IFN-alpha 2c

Epitope

43aa-53aa, outside the immunodominant regions I and II

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Specificity

This antibody is specific for human IFN-alpha 2 and does not cross react with human IFN-alpha 1. It reacts with linear peptide 43aa-53aa, placing the epitope outside the immunodominant regions I and II.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for IFN-alpha 2 Antibody (N27) - BSA Free

Western Blot: IFN-alpha 2 Antibody (N27) [NBP3-18803]

Western Blot: IFN-alpha 2 Antibody (N27) [NBP3-18803]

Western Blot: IFN-alpha 2 Antibody (N27) [NBP3-18803] - Western blot using IFN-alpha 2 antibody (N27)

Applications for IFN-alpha 2 Antibody (N27) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Frozen

1-2 ug/ml

Western Blot

1:100-1:2000
Application Notes
N27 is one of many MoAbs described by Kontsek et al. (1991) directed against a site different from the two immunodominant sites called I and II. As such it is against an "atypical" site of the molecule. Peptide mapping for N27 has been done by Kontsek et al. Not recommended for IHC-paraffin sections.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IFN-alpha 2/IFNA2

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of twoenzymes: a protein kinase and an oligoadenylate synthetase

Long Name

Interferon alpha 2

Alternate Names

Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2

Gene Symbol

IFNA2

Additional IFN-alpha 2/IFNA2 Products

Product Documents for IFN-alpha 2 Antibody (N27) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for IFN-alpha 2 Antibody (N27) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

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Protocols

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FAQs

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