IgA Antibody (7D5F12) - BSA Free

Novus Biologicals | Catalog # NBP2-61794

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7D5F12

Format

BSA Free
View all IgA Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human IgA (AA: 207-353) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IgA Antibody (7D5F12) - BSA Free

Western Blot: IgA Antibody (7D5F12)BSA Free [NBP2-61794]

Western Blot: IgA Antibody (7D5F12)BSA Free [NBP2-61794]

Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against HEK293 (1) and IghA1 (AA: 207-353)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

Immunocytochemistry/ Immunofluorescence: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

Immunocytochemistry/Immunofluorescence: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

Immunohistochemistry-Paraffin: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of breast cancer tissues using IghA1 mouse mAb with DAB staining.
Flow Cytometry: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

Flow Cytometry: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

Flow Cytometry: IgA Antibody (7D5F12) [NBP2-61794] - Analysis of Hela cells using IghA1 mouse mAb (green) and negative control (red).
Western Blot: IgA Antibody (7D5F12)BSA Free [NBP2-61794]

Western Blot: IgA Antibody (7D5F12)BSA Free [NBP2-61794]

Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mAb against human IghA1 (AA: 207-353) recombinant protein. (Expected MW is 41.7 kDa)
Western Blot: IgA Antibody (7D5F12)BSA Free [NBP2-61794]

Western Blot: IgA Antibody (7D5F12)BSA Free [NBP2-61794]

Western Blot: IgA Antibody (7D5F12) [NBP2-61794] - Analysis using IghA1 mouse mAb against L1210 (1), THP-1 (2), HepG2 (3), and COS7 (4) cell lysate.
ELISA: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

ELISA: IgA Antibody (7D5F12) - BSA Free [NBP2-61794]

ELISA: IgA Antibody (7D5F12) [NBP2-61794] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for IgA Antibody (7D5F12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgA

IGHA1 (Immunoglobulin Heavy Constant Alpha 1) is a Protein Coding gene. Diseases associated with IGHA1 include pseudotumor cerebri. Among its related pathways are Vesicle-mediated transport and Regulation of nuclear SMAD2/3 signaling. GO annotations related to this gene include antigen binding and immunoglobulin receptor binding. An important paralog of this gene is IGHG4.;

Long Name

Immunoglobulin A

Alternate Names

Immunoglobulin A

Gene Symbol

IGHA1

Additional IgA Products

Product Documents for IgA Antibody (7D5F12) - BSA Free

Certificate of Analysis

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Product Specific Notices for IgA Antibody (7D5F12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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