IgA Antibody (RM128)

Novus Biologicals | Catalog # NBP2-62023

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM128 expressed in HEK293
View all IgA Primary Antibodies »

Product Specifications

Immunogen

Human IgA

Specificity

This antibody reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgA Antibody (RM128)

Immunohistochemistry: IgA Antibody (RM128) [Unconjugated] [NBP2-62023] - Immunohistochemistry of human Lymphoid Tissue using NBP2-62023.
IgA Antibody (RM128)

Sandwich ELISA: IgA Antibody (RM128) [NBP2-62023] -

Sandwich ELISA: IgA Antibody (RM128) [NBP2-62023] - Sandwich ELISA using RM128 as the capture antibody (100ng/well), and Biotinylated RM129 (NBP3-18532) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
IgA Antibody (RM128)

ELISA: IgA Antibody (RM128) - Azide and BSA Free [NBP2-62023] -

ELISA: IgA Antibody (RM128) - Azide and BSA Free [NBP2-62023] - A titer ELISA using RM128. The plate was coated with different amounts of human IgA. A serial dilution of RM128 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgA Antibody (RM128)

Sandwich ELISA: IgA Antibody (RM128) - Azide and BSA Free [NBP2-62023] -

Sandwich ELISA: IgA Antibody (RM128) - Azide and BSA Free [NBP2-62023] - Sandwich ELISA using NBP2-62023 as the capture antibody (100ng/well), and biotinylated NBP3-18532 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
IgA Antibody (RM128)

ELISA: IgA Antibody (RM128) - Azide and BSA Free [NBP2-62023] -

ELISA: IgA Antibody (RM128) - Azide and BSA Free [NBP2-62023] - ELISA of human immunoglobulins shows RM128 reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50ng/mL, or 10 ng/mL of NBP3-25969 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgA Antibody (RM128)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.5 - 2ug/ml

Immunohistochemistry

0.1 - 1ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgA

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The most common feature of plasmacytomas, and certain non Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non Hodgkin's lymphomas.

Long Name

Immunoglobulin A

Alternate Names

Immunoglobulin A

Gene Symbol

IGHA1

Additional IgA Products

Product Documents for IgA Antibody (RM128)

Certificate of Analysis

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Product Specific Notices for IgA Antibody (RM128)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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