Goat anti-Canine IgE Heavy Chain Secondary Antibody
Novus Biologicals | Catalog # NB7343
![ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]](https://resources.rndsystems.com/images/products/Goat-anti-Canine-IgE-Secondary-Antibody-ELISA-NB7343-img0001.jpg "ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]")
Key Product Details
Species Reactivity
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Immunogen
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Scientific Data Images for Goat anti-Canine IgE Heavy Chain Secondary Antibody
ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343]
ELISA: Goat anti-Canine IgE Heavy Chain Secondary Antibody [NB7343] - Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations. Coating: 1ug/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with secondary antibody (HRP-labeled) and TMB substrate. This image was submitted via customer Review.
Applications for Goat anti-Canine IgE Heavy Chain Secondary Antibody
ELISA
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
Reviewed Applications
Read 2 reviews rated 5 using NB7343 in the following applications:
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Background: IgE Heavy Chain
Alternate Names
Additional IgE Heavy Chain Products
Product Documents for Goat anti-Canine IgE Heavy Chain Secondary Antibody
Certificate of Analysis
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Product Specific Notices for Goat anti-Canine IgE Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Goat anti-Canine IgE Heavy Chain Secondary Antibody
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Customer Images
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Application: ELISASample Tested: 293T cellsSpecies: CanineVerified Customer | Posted 02/05/2019
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Verified Customer | Posted 01/31/2017Sandwich ELISA for total dog IgE measurement showing standard curve at 2 different coating antibody concentrations.We regularly use this antibody as capture antibody to measure total dog IgE from serum and supernatants in ELISA. Coating: 1µg/ml in carbonate buffer, pH 9.6. Standard: purified dog IgE, range: 156pg/ml to 15.000 pg/ml. Detection with NB7346 (HRP-labeled) and TMB substrate.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Goat anti-Canine IgE Heavy Chain Secondary Antibody
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Q: I wanted to ask if it is possible to order the goat anti-canine IgE (NB7343) in a customized way labeled with Cy3 for example and if it is possible how much will it cost? Thank you for your information.
A: Unfortunately, we do not have the license to the Cy3 dye, as it is still under patent. This antibody can be custom conjugated to DyLight 550, however, which is a more stable, Cy3 equivalent. Usually the cost for custom labeling is around $80. You can request a quote by filling out the form.
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Q: Is it also possible to label the antibody with AlexaFluor 532?
A: We do not have a license for the Alexa Fluor dyes at this time. The DyLight dyes are almost an exact equivalent. DyLight 550 is the closest to Cy3 that we have to offer for conjugation currently.
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Q: I wanted to ask if it is possible to order the goat anti-canine IgE (NB7343) in a customized way labeled with Cy3 for example and if it is possible how much will it cost? Thank you for your information.
A: Unfortunately, we do not have the license to the Cy3 dye, as it is still under patent. This antibody can be custom conjugated to DyLight 550, however, which is a more stable, Cy3 equivalent. Usually the cost for custom labeling is around $80. You can request a quote by filling out the form.
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Q: Is it also possible to label the antibody with AlexaFluor 532?
A: We do not have a license for the Alexa Fluor dyes at this time. The DyLight dyes are almost an exact equivalent. DyLight 550 is the closest to Cy3 that we have to offer for conjugation currently.