IGF-I R/IGF1R Antibody (3G5C1)

Novus Biologicals | Catalog # NB110-87052

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3G5C1
View all IGF-I R/IGF1R Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of IGF-I R/IGF1R (AA: 1101-1367) expressed in E. Coli.

Localization

Membrane; Single-pass type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGF-I R/IGF1R Antibody (3G5C1)

Western Blot: IGF-I R/IGF1R Antibody (3G5C1)BSA Free [NB110-87052]

Western Blot: IGF-I R/IGF1R Antibody (3G5C1)BSA Free [NB110-87052]

Western Blot: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis using IGF1R-Beta mouse mAb against truncated IGF1R recombinant protein.
Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

IGF-I-R-IGF1R-Antibody-3G5C1-Immunohistochemistry-NB110-87052-img0022.jpg
Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry-Paraffin: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis of ovarian cancer tissues using IGF1R-Beta mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052]

Immunohistochemistry-Paraffin: IGF-I R Antibody (3G5C1) [NB110-87052] - Analysis of rectum cancer tissues using IGF1R-Beta mouse mAb with DAB staining.
IGF-I R/IGF1R Antibody (3G5C1)

Immunohistochemistry: IGF-I R/IGF1R Antibody (3G5C1) [NB110-87052] -

Expression of growth factor receptors in male breast cancer.Expression of HER2, FGFR2, EGFR, IGF1-R, and MET in representative cases of male breast cancer. Size bars equal 20 µm.
IGF-I R/IGF1R Antibody (3G5C1)

Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) [NB110-87052] -

Representative IHC staining in primary breast tumors (left; no membrane staining for Claudin-4, CAIX and GLUT-1 (significant negative to positive conversion), positive membrane staining for EGFR and IGF1R (negative to positive conversion n.s.) and metastases (right; positive membrane staining). Magnification: 20× (inlet 40×).
IGF-I R/IGF1R Antibody (3G5C1) - BSA Free

Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052] -

Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (3G5C1) - BSA Free [NB110-87052] - Representative IHC staining in primary breast tumors (left; no membrane staining for Claudin-4, CAIX & GLUT-1 (significant negative to positive conversion), positive membrane staining for EGFR & IGF1R (negative to positive conversion n.s.) & metastases (right; positive membrane staining). Magnification: 20× (inlet 40×). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25417118), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for IGF-I R/IGF1R Antibody (3G5C1)

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 2 reviews rated 4.5 using NB110-87052 in the following applications:

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGF-I R/IGF1R

IGF1 Receptor (insulin-like growth factor 1 receptor, IGF1R), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF1 and IGF2, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

Long Name

Insulin-like Growth Factor I Receptor

Alternate Names

CD221, IGF-1R, IGF-IR, IGF1R, IGFIR

Entrez Gene IDs

3480 (Human)

Gene Symbol

IGF1R

UniProt

P08069

Additional IGF-I R/IGF1R Products

Product Documents for IGF-I R/IGF1R Antibody (3G5C1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IGF-I R/IGF1R Antibody (3G5C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IGF-I R/IGF1R Antibody (3G5C1)

Customer Reviews for IGF-I R/IGF1R Antibody (3G5C1) (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • IGF-I R Antibody (3G5C1)
    Name: Paula Aliberti
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Infant testis
    Species: Rhesus Macaque
    Verified Customer | Posted 11/24/2017
    Specific staining on germ cells. Very nice staining. Ab dilution: 1/100
    IGF-I R/IGF1R Antibody (3G5C1) NB110-87052
  • IGF-I R Antibody (3G5C1)
    Name: Paula Aliberti
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human testis
    Species: Human
    Verified Customer | Posted 10/09/2017
    Fetal human testis 40x. High IGF1R immunoexpression in fetal Leydig cells.
    antibody dilution 1/50
    IGF-I R/IGF1R Antibody (3G5C1) NB110-87052

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Protocols

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FAQs for IGF-I R/IGF1R Antibody (3G5C1)

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  • Q: I ordered NB110-87052 and the website said that the concentration is mentioned on the vial, but it is not. Can you tell me what's the (antibody) concentration in Lot 81210 and 3G5C1?

    A: The formulation for the product is ascites fluid therefore the concentration is unknown because we cannot typically measure an accurate concentration with ascites that hasn't been purified.

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