IGF-II R/IGF2R Antibody (2G11) - BSA Free

Novus Biologicals | Catalog # NB300-514

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G11

Format

BSA Free
View all IGF-II R/IGF2R Primary Antibodies »

Product Specifications

Immunogen

Purified bovine Mannose 6 Phosphate Receptor (Cation independent) [UniProt# P08169]

Epitope

In the extracellular domain of Mannose 6 Phosphate Receptor (Cation independent).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9119492). Does not react with Chinese hamster ovary cell Mannose 6 Phosphate Receptor (PMID: 8163521).

Marker

Late Endosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for IGF-II R/IGF2R Antibody (2G11) - BSA Free

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-IGF-II R/IGF2R Antibody (2G11) NB300-514 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunohistochemistry: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunohistochemistry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining of human testis using NB300-514 antibody at 1:100.
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mannose 6-Phosphate Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - HMVEC Cells
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining in Hela Cells. Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Mannose 6-Phosphate Receptor at a dilution of 1:20 over night at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody.
Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/ Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 Phosphate Receptor (Cation independent) antibody (2G11) was tested at (1:250) in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight-550 (red).
Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) - BSA Free [NB300-514]

Flow Cytometry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Mannose 6 Phosphate Receptor antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Applications for IGF-II R/IGF2R Antibody (2G11) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:250

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Western Blot

1 ug/ml
Application Notes
By Western Blot, a 300 kDa protein representing Mannose 6 Phosphate Receptor is seen in HeLa cell extract under non-reducing conditions. Immunofluorescence staining of Mannose 6 Phosphate Receptor in HeLa cells with this antibody results in perinuclear staining. This antibody is also useful in Flow Cytometry, Immunoprecipitation, ELISA and Immunohistochemistry-on paraffin embedded sections. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IGF-II R/IGF2R

Mannose 6 phosphate is a sequence tag that plays a pivotal role in delivering proteins to the lysosome. Specifically, the mannose 6 phosphate sequence is tagged to N-linked oligosaccharides of lysosomal hydrolases as they pass through the cis-Golgi network. Once the lysosomal enzymes are tagged with the M6P sequence, they are destined to the late endosome by way of vesicular transport. After a low pH dissociates the M6P Receptor from its ligand, the M6P sequences are recycled. Additionally, M6P is bound by lectin in the immune system, and is converted to fructose 6-phosphate by mannose phosphate isomerase.

Long Name

Insulin-like Growth Factor II Receptor

Alternate Names

CD222, CIMPR, IGF-IIR, IGF2R, IGFIIR, M6P-R, MPRI

Entrez Gene IDs

3482 (Human); 16004 (Mouse); 25151 (Rat); 281849 (Bovine)

Gene Symbol

IGF2R

UniProt

P08169 (Bovine)

Additional IGF-II R/IGF2R Products

Product Documents for IGF-II R/IGF2R Antibody (2G11) - BSA Free

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Product Specific Notices for IGF-II R/IGF2R Antibody (2G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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