Goat anti-Human IgG F(ab')2 Secondary Antibody [HRP]

Product Specific Notices for Goat anti-Human IgG F(ab')2 Secondary Antibody [HRP]

Antiserum was solid phase adsorbed to ensure specificity. The antibody to human F(ab')2 was isolatedby affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generatedusing a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species. Suggested starting dilutions are: ELISA Colorimetric: 1:2000 - 1:20000, Chemiluminescent: 1:5000 - 1:25000

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.