Key Product Details

Species Reactivity

Bat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

This Goat anti-Bat IgG (H+L) Secondary Antibody was developed against bat IgG-heavy and light chain.

Specificity

By immunoelectrophoresis and ELISA this Goat anti-Bat IgG (H+L) Secondary Antibody reacts specifically with Bat IgG and with light chains common to other Bat immunoglobulins. Antibody has been shown to react with bat genus species Pteropus vampirus, Desmodus rotundus, Eptesicus fuscus, Tadrida pumila, T. condylura, Hypsignathus monstrosus, Rosettus aegyptiacus, Epomorphus crypturus, Molossus species. And Phylostomus species. No antibody was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237]

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237]

Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0002.jpg
Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237]

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237]

Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0001.jpg
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Intracellular & surface Ig staining of P. alecto splenocytes. Dead cells were excluded using Live/Dead eFluor 506 dye & live cells were gated for singlets using forward scatter height (FSC-H) & area (FSC-A). For all the staining approaches (surface only, intracellular only, or surface + intracellular), a distinct sIg+ CD3+/− population is observed. Greater than 85% of this population is MHCII+. Comparable percentages of these cells were obtained with surface staining only vs. intracellular staining only, thus strongly suggesting that this population is truly a sIg+ B cell population. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30930908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Goat anti-Bat IgG (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] -

Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Goat anti-Bat IgG (H+L) Secondary Antibody

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200- 1:2000

Immunohistochemistry

1:200- 1:2000

Immunohistochemistry-Paraffin

1:200-1:2000

Western Blot

1:1000 - 1:30000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Bat IgG (H+L) Secondary Antibody

Certificate of Analysis

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Product Specific Notices for Goat anti-Bat IgG (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Bat IgG (H+L) Secondary Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Goat anti-Bat IgG (H+L) Secondary Antibody

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  • Q: I want to do an ELISA assay on your bat IgG antibody, but on indigenous species. Do you know if it will bind to the IgGs? And can I use the unconjugated ab for coating (?) and your HRP conjugated one as detection ab?

    A: They should coat with NB7237 and detect with NB7238. NB7237 will detect bat genus species Pteropus vampirus, Desmodus rotundus, Eptesicus fuscus, Tadrida pumila, T. condylura, Hypsignathus monstrosus, Rosettus aegyptiacus, Epomorphus crypturus, Molossus species, and Phylostomus species. No antibody was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species. NB7238 will detect bat IgG, but may cross react with IgG from other species.

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