Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IgG (H+L) Secondary Antibodies »

Product Specifications

Immunogen

This Goat anti-Human IgG (H+L) Secondary Antibody was developed against human IgG-heavy and light chain.

Specificity

By immunoelectrophoresis and ELISA this Goat anti-Human IgG (H+L) Secondary Antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Goat anti-Human IgG (H+L) Secondary Antibody

Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475]

Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475]

Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475] - This Goat anti-Human IgG (H+L) Secondary Antibody conjugated with pHAb Dyes which have excitation maxima (Ex ) at 532nm and emission maxima (Em) at 560nm. Used this with primary antibody of interest to visualize cells and study Internalisation using microscope. This image was submitted via customer review.
Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475]

Immunocytochemistry/ Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475]

Immunocytochemistry/Immunofluorescence: Goat anti-Human IgG (H+L) Secondary Antibody [NB7475] - Human SKCO1 cells. Image from verified customer review. Image using the FITC form of Goat anti-Human IgG (H+L) Secondary Antibody.

Applications for Goat anti-Human IgG (H+L) Secondary Antibody

Application
Recommended Usage

ELISA

1:1000-1:30000(Primary) 1:100-1:500(for coating plates)

Immunocytochemistry/ Immunofluorescence

1:200- 1:2000

Immunohistochemistry

1:200- 1:2000

Immunohistochemistry-Paraffin

1:200- 1:2000

Western Blot

1:1000-1:30000

Reviewed Applications

Read 2 reviews rated 5 using NB7475 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

Product Documents for Goat anti-Human IgG (H+L) Secondary Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Goat anti-Human IgG (H+L) Secondary Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Goat anti-Human IgG (H+L) Secondary Antibody

Customer Reviews for Goat anti-Human IgG (H+L) Secondary Antibody (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Goat anti-Human IgG (H+L) Secondary Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: 3 human cancer cell lines
    Species: Human
    Verified Customer | Posted 02/14/2018
    Conjugated this antibody with pHAb Dyes which have excitation maxima (Ex ) at 532nm and emission maxima (Em) at 560nm. Used this with primary antibody of interest to visualize cells and study Internalisation using microscope
    Goat anti-Human IgG (H+L) Secondary Antibody NB7475
  • Goat anti-Human IgG (H+L) Secondary Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Cell culture
    Species: Human
    Verified Customer | Posted 02/13/2018
    I conjugated the antibody with PH sensitive dyes and used for my cell Internalisation studies on microscope. It works very well. Highly recommended obtained high yield after conjugation and nice Images

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Protocols

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FAQs

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