Rabbit IgG Isotype Control
Novus Biologicals | Catalog # NBP2-36463
![Western Blot: Rabbit IgG Isotype Control [NBP2-36463]](https://beta-resources.rndsystems.com/images/products/Rabbit-IgG-Isotype-Control-Western-Blot-NBP2-36463-img0028.jpg "Western Blot: Rabbit IgG Isotype Control [NBP2-36463]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Rabbit
Applications
Immunohistochemistry, Western Blot, ELISA, Flow (Intracellular), Chromatin Immunoprecipitation (ChIP), SDS-Page
Label
Unconjugated
Antibody Source
Rabbit IgG
Format
Purified
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Product Specifications
Host
Rabbit
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Rabbit IgG Isotype Control
Western Blot: Rabbit IgG Isotype Control [NBP2-36463]
Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.![Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463]](https://beta-resources.rndsystems.com/images/products/Rabbit-IgG-Isotype-Control-Flow-Intracellular-NBP2-36463-img0007.jpg "Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463]")
Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463]
Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463] - Intracellular staining for GLI1, GLI2 and GLI3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using a secondary anti-rabbit IgG PE conjugated antibody. Image courtesy of Sherine Elsawa, Northern Illinois University.![SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]](https://beta-resources.rndsystems.com/images/products/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0018.jpg "SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]")
SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]
SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa, Reduced at 55, 25 kDa.![SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]](https://beta-resources.rndsystems.com/images/products/Rabbit IgG Isotype Control-SDS-Page-NBP2-36463-img0030.jpg "SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]")
SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]
SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - SDS-Page of Rabbit IgG. Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG. Other Band(s): None.![SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]](https://beta-resources.rndsystems.com/images/products/Rabbit-IgG-Isotype-Control-SDS-Page-NBP2-36463-img0029.jpg "SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]")
SDS-PAGE: Rabbit IgG Isotype Control [NBP2-36463]
SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.Applications for Rabbit IgG Isotype Control
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
SDS-Page
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Use in ChIP reported in scientific literature (PMID:34335948).
Use in ChIP reported in scientific literature (PMID:34335948).
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for Rabbit IgG Isotype Control
Product Specific Notices for Rabbit IgG Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
Citations for Rabbit IgG Isotype Control
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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