Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Novus Biologicals | Catalog # NBP1-72793
![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.rndsystems.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0005.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Dot Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Mouse IgG1 heavy chain
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.![Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.rndsystems.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72793-img0011.jpg "Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Immunocytochemistry/ Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
Immunocytochemistry/Immunofluorescence: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - DyLight (R) dyes can be used for multi-color immunofluorescence microscopy with uniform fluorescence intensity throughout the image. Image using the DyLight 488 format of this antibody.![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.rndsystems.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72793-img0001.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.
Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng.
Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Mouse IgG1. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng.
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.rndsystems.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72793-img0006.jpg "Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - Fluorescence absorption and emission spectra of Dylight 800 in PBS, pH 7.2.
Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
![ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]](https://resources.rndsystems.com/images/products/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72793-img0012.jpg "ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]")
ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793]
ELISA: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [NBP1-72793] - ELISA results of purified Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody tested against purified Mouse IgG1. Each well was coated in duplicate with 1.0 ug of Mouse IgG1. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Goat anti-Rabbit HRP, and TMB substrate.
Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Dot Blot of Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody. Tested against dilutions 10ng, 3.3ng, 1.1ng, 0.37ng, 0.12ng. Lane 1: Mouse IgG1Applications for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product is available in a variety of formats. Anti-Mouse IgG1 Antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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