Mouse IgG2b Isotype Control (M2B) [Rhodamine]

Novus Biologicals | Catalog # NBP1-96851

Novus Biologicals

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Rhodamine (Excitation = 570 nm, Emission = 590 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # M2B
View all IgG2B Isotype Controls »

Product Specifications

Specificity

Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Rhodamine Conjugated Mouse IgG2b was proven by Dot Blot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Rhodamine Conjugated Mouse IgG2b was proven by Dot Blot.

Applications for Mouse IgG2b Isotype Control (M2B) [Rhodamine]

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:1000 - 1:5000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This product can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2b Isotype Control (M2B) [Rhodamine]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mouse IgG2b Isotype Control (M2B) [Rhodamine]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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