Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, SDS-Page

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # M2B

Format

Purified
View all IgG2B Isotype Controls »

Product Specifications

Specificity

Extensive cross-adsorption was performed to remove any unwanted subclasses. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Confirmed by SDS-PAGE.

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Mouse IgG2b Isotype Control (M2B)

SDS-PAGE: Mouse IgG2b Isotype Control (M2B) [NBP1-96969]

SDS-PAGE: Mouse IgG2b Isotype Control (M2B) [NBP1-96969]

SDS-Page: Mouse IgG2b Isotype Control (M2B) [NBP1-96969] - SDS-PAGE results of Mouse IgG2b isotype control (M2B).Lane 1: reduced Mouse IgG2b isotype control (M2B).Lane 2: opal prestained molecular weight ladder.Lane 3: non-reduced Mouse IgG2b isotype control (M2B).Load: 1ug.4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.

Applications for Mouse IgG2b Isotype Control (M2B)

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:1000 - 1:5000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Purified

Preservative

0.01% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2b Isotype Control (M2B)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Mouse IgG2b Isotype Control (M2B)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mouse IgG2b Isotype Control (M2B)

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  • Q: Could you advise if I can use NBP1-96969 Ab as a control Ab for HIF1a for ChIP assay. I have a problem that normal rabbit IgG that usually are provided in the ChIP kits show higher binding compare to NB100-105 HIF1A Ab. Could you suggest if it is better to use Mouse IgG 2b Isotype control in this case or normal rabbit IgG should work as well? NBP1-96969 Ab are not validated for ChIP, please, let me know if there is something better? 

    A: Yes, it would be reasonable to use a mouse IgG2b control for NB100-105 (HIF1 alpha) in ChIP assay. The control provided in the ChIP kit is recommended as a control for the histone antibodies (which are rabbit polyclonal). NBP1-96969 would be a good choice for control for the mouse monoclonal NB100-105. It has not yet been validated for ChIP, but it has been validated for use in IP, so I think it will be a good choice for control.

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View all FAQs for Isotype Controls