IgG4 Antibody (RM120) [Biotin]

Novus Biologicals | Catalog # NBP2-62126B

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM120 expressed in HEK293
View all IgG4 Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to the hinge region of Human IgG4

Specificity

This antibody reacts to the heavy chain of human IgG4, This antibody does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG4 Antibody (RM120) [Biotin]

IgG4 Antibody (RM120) [Biotin]

ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] -

ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Sandwich ELISA using NBP2-62126B as the capture antibody, and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.
IgG4 Antibody (RM120) [Biotin]

ELISA: IgG4 Antibody (RM120) - Azide and BSA Free [NBP2-62126B] -

ELISA: IgG4 Antibody (RM120) - Azide and BSA Free [NBP2-62126B] - A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
IgG4 Antibody (RM120) [Biotin]

ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] -

ELISA: Rabbit anti-Human IgG4 Secondary Antibody (RM120) [Biotin] [NBP2-62126B] - Sandwich ELISA using NBP2-62126B as the capture antibody, and Biotinylated anti-human light chains (kappa + lambda) antibody as the detection antibody, followed by an AP conjugated streptavidin.

Applications for IgG4 Antibody (RM120) [Biotin]

Application
Recommended Usage

Flow Cytometry

1ug/ml - 4ug/ml

Immunocytochemistry

1ug/mL - 10ug/mL

Immunocytochemistry/ Immunofluorescence

1 - 10 ug/ml

Immunohistochemistry

1ug/mL - 10ug/mL

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml

Western Blot

0.5 ug/ml - 2 ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS with 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG4

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G4

Alternate Names

Immunoglobulin G, Immunoglobulin G4, ImmunoglobulinG

Additional IgG4 Products

Product Documents for IgG4 Antibody (RM120) [Biotin]

Certificate of Analysis

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Product Specific Notices for IgG4 Antibody (RM120) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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