IgG4 Antibody (RM120) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25977

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM120 expressed in HEK293

Format

Azide and BSA Free
View all IgG4 Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to the hinge region of Human IgG4

Specificity

This antibody reacts to the heavy chain of human IgG4, This antibody does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IgG4 Antibody (RM120) - Azide and BSA Free

IgG4 Antibody (RM120) - Azide and BSA Free

Western Blot: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] -

Western Blot: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.
IgG4 Antibody (RM120) - Azide and BSA Free

Immunohistochemistry-Paraffin: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] -

Immunohistochemistry-Paraffin: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - Immunohistochemistry of Human Lymphoid Tissue using Clone RM120.
IgG4 Antibody (RM120) - Azide and BSA Free

ELISA: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] -

ELISA: IgG4 Antibody (RM120) - Azide and BSA Free [NBP3-25977] - A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Applications for IgG4 Antibody (RM120) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1ug/ml-4ug/ml

Immunocytochemistry/ Immunofluorescence

1 - 10 ug/ml

Immunohistochemistry

1 - 10 ug/ml

Immunohistochemistry-Paraffin

1 - 10 ug/ml

Sandwich ELISA Capture

50 - 200ng/well

Sandwich ELISA Detection

0.05 - 0.2ug/ml

Western Blot

0.5 ug/ml - 2 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IgG4

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G4

Alternate Names

Immunoglobulin G, Immunoglobulin G4, ImmunoglobulinG

Additional IgG4 Products

Product Documents for IgG4 Antibody (RM120) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IgG4 Antibody (RM120) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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